Isolation and Characterization of Two Distinct Gonadotropins from the Pituitary Gland of Mediterranean Yellowtail,Seriola dumerilii(Risso, 1810)

Molecular characterization of Chinese sturgeon gonadotropins and cellular distribution in pituitaries of mature and immature individuals

“…Our results suggested that the expression pattern of Chinese sturgeon LH␤ might be similar to that observed in salmonids. In teleosts, the duality of FSH␤ and LH␤ was first revealed by Kawauchi et al (1989) and has been demonstrated in a number of species (Koide et al, 1993;Okada et al, 1994;Hassin et al, 1995;Elizur et al, 1996;García-Hernández et al, 1997;Kagawa et al, 1998;Calman et al, 2001;Mateos et al, 2003;Li et al, 2005;Cerdà et al, 2008). However, it is still unclear whether the FSH␤ and LH␤ are synthesized in the same cells as it occurs in tetrapods (Saito et al, 2002).…”

CARACTERIZACIÓN BIOLÓGICA DE LAS GONADOTROFINAS DE LUBINA (Dicentrarchus labrax) Y DESARROLLO DE HERRAMIENTAS BIOTECNOLÓGICAS E INMUNOLÓGICAS PARA SU ESTUDIO

“…Intact sbsFSH eluted mainly when the linear gradient achieved the 170 mM ammonium bicarbonate on the ion-exchange column, which is comparable to the 200 mM observed for coho salmon , bonito (Koide et al, 1993), Mediterranean yellowtail (García- Hernández et al, 1997), and Atlantic halibut . In contrast, FSH from tuna (Okada et al, 1994) eluted with 300 mM ammonium bicarbonate, which indicates that it has a higher negative charge.…”

“…There is a great variability in the molecular weight of those reported so far, finding subunits that range from 15 to 28 KDa and intact FSH from 32 to 50 KDa Van der Kraak et al, 1992;Koide et al, 1993;Copeland and Thomas, 1993;Okada et al, 1994;García-Hernández et al, 1997;Govoroun et al, 1997;Kamei et al, 2005). According to the predicted amino acid sequences, the contribution of the polypeptide backbone to the molecular weight is similar among different teleost species, therefore the different molecular weight estimates could be due to differences in the glycosylation degree of the subunits or a consequence of the method of mass determination employed.…”

“…In some teleosts such as chum salmon, coho salmon and Mediterranean yellowtail, it is accepted the existence of two types of FSH that are chemically different: one acid-stable type and another acid-unstable type García-Hernández et al, 1997). This unusual stability to acid treatment could suggest that its subunits are linked by disulfide bonds .…”

“…Traditionally, the protocols used in the purification of teleost GTH dimers included a combination of gel filtration and ion-exchange chromatography Van der Kraak et al, 1992;Copeland and Thomas, 1993;Kamei et al, 2005). Nevertheless, alternative protocols using ion-exchange and reverse phase chromatography under neutral or slightly basic conditions have been described for the purification of FSH and LH from other teleost species including the bonito (Koide et al, 1993), tuna (Okada et al, 1994), Mediterranean yellowtail (García- Hernández et al, 1997) and Atlantic halibut . In this study we used anion-exchange and reverse phase chromatography under slightly basic conditions and it turned out to be particularly effective for the isolation of sbsFSH.…”