Isolation and characterization of Vibrio owensii from Palk Bay and its infection study against post larvae of Litopenaeus vannamei

Muneeswaran, Thillaichidambaram; Kalyanaraman, Narayanan; Selvaraj, Sureshkumar; Sundararaju, Sathyavathi; Ramakritinan, C.M.; Figge, Marian J.

doi:10.1016/j.micpath.2022.105751

Cited by 3 publications

(2 citation statements)

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“…Recently, V owensii has been reported to be resistant to aztreonam, streptomycin, oxacillin, tetracycline, and minocycline; and intermediate resistant to ampicillin, piperacillin, and cefotaxime (Dai et al 2022). Thillaichidambaram et al (2022) also reported that V. owensii isolated from the Palk Bay of India is highly resistant to ampicillin, methicillin, tetracycline and vancomycin. This study and the previous publications suggest that V. owensii possesses a wide range of antibiotic resistance properties.…”

Section: Discussionmentioning

confidence: 99%

“…V. owensii is also a pathogenic in marine aquaculture with a high rate of resistance to antibiotics. This Vibrio species is resistant to ampicillin, erythromycin, kanamycin, ofloxacin, rifampicin, methicillin, tetracycline and vancomycin (Thillaichidambaram et al 2022). reported that V. owensii isolated from Fenneropenaeus chinensis is resistant to 18 antibiotics among the 30 antibiotics tested.…”

Section: Introductionmentioning

confidence: 99%

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Identification and antibiotic-resistant properties of Vibrio owensii and V. alginolyticus isolated from the Spermonde Islands, Indonesia

et al. 2022

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Abstract. Isnansetyo A, Istiqomah I, Anshary H, Sriwulan S, Yudiati E, Subagiyo S, Arif A, Kartikasari DW. 2022. Identification and antibiotic-resistant properties of Vibrio owensii and V. alginolyticus isolated from the Spermonde Islands, Indonesia. Biodiversitas 23: 5995-6005. The Spermonde Islands are located in Makassar Strait, southern Sulawesi, Indonesia, and have attracted the attention of researchers for decades because of their mega biodiversity and abundance of bioresources. However, no study has evaluated the potential mariculture diseases in this area. The present study assessed the potential bacterial fish diseases based on the current status of mariculture in the Spermonde Islands. The samples were collected from three marine aquaculture sites at Barrang Caddi and the Samaloa Islands. Vibrio was isolated on Thiosulfate-citrate-bile-sucrose (TCBS) agar medium. The most common bacterial diseases were observed by bacterial isolation, morphological and biochemical observations, and molecular identification based on 16S rDNA sequence. The microbial, physical, and chemical water qualities at Barrang Caddi and Samaloa Island were suitable for marine aquaculture. Although cultured species in this area did not exhibit gross disease signs, potential opportunistic pathogens of Vibrio were found. The 16S rDNA sequences analysis indicated that the bacterial isolates closed to Vibrio owensii, V. alginolyticus, and V. neocaledonius. Multi-drug resistant V. owensii was identified as a potential pathogen in marine aquaculture. This is the first report on V. owensii isolated from Indonesian marine waters, particularly from the Spermonde Islands. V. alginolyticus was another potential opportunistic pathogen in this area. These results gave the alarm to develop countermeasure methods for potential diseases to minimize the possible outbreak of vibriosis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification and antibiotic-resistant properties of Vibrio owensii and V. alginolyticus isolated from the Spermonde Islands, Indonesia

et al. 2022

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Effects of a phytobiotic-based additive on the growth, hepatopancreas health, intestinal microbiota, and Vibrio parahaemolyticus resistance of Pacific white shrimp, Litopenaeus vannamei

Ma,

Zhao,

Liu

et al. 2024

Front. Immunol.

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Vibrio genus is a common pathogen in aquaculture and causes acute hepatopancreatic necrosis disease (AHPND) and massive mortality of shrimp. Many studies have suggested that a single functional ingredient such as plant extract or organic acid can reduce the dependence on antibiotics and promote the growth and immunity of aquatic animals. In this study, we evaluated the effects of a phytobiotic-based compound additive (Sanacore® GM, SNGM), which had a successful trajectory of commercial application in fish farming. However, its effects on the hepatopancreas health and intestinal microbiota of shrimp after Vibrio challenge have not been well evaluated. In the present study, Pacific white shrimp were fed diets with or without supplementation of SNGM, and the SNGM grades were 0-g/kg (CON), 3-g/kg (SNGM3), and 5-g/kg (SNGM5) diets. The feed trial lasted 60 days, after which a Vibrio parahaemolyticus challenge was performed. The results showed that compared to the CON group, both the SNGM3 and SNGM5 groups had a significantly higher weight gain and a lower feed conversion ratio as well as higher survival after Vibrio parahaemolyticus challenge. In the growth trial, the SNGM3 group had a significantly increased total protein, albumin concentration, and acid phosphatase activity in hemolymph compared to the CON group. In the challenge experiment, the SNGM3 and SNGM5 groups had increased albumin and glucose contents as well as the activities of phenoloxidase, lysozyme, alkaline phosphatase, and superoxide dismutase in hemolymph. Both the SNGM3 and SNGM5 groups had improved morphology of the hepatopancreas and intestine. The SNGM5 group had alleviated gut microbiota dysbiosis induced by Vibrio infection by increasing the potential probiotic bacterium abundance (Shewanella) and decreasing the potential pathogenic bacteria abundance (Vibrio, Photobacteriuma, Pseudoalteromonas, and Candidatus_Bacilloplasma). In conclusion, the dietary phytobiotic-based additive at 3-g/kg level increased the growth and Vibrio parahaemolyticus resistance of Pacific white shrimp by promoting immune-related enzyme activities and improving the morphological structure of the hepatopancreas and intestine and the intestinal microbiota composition.

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Antibiotic Resistance in Vibrio Bacteria Associated with Red Spotting Disease in Sea Urchin Tripneustes gratilla (Echinodermata)

Ben Natan,

Masasa,

Shashar

et al. 2024

Microorganisms

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The red spotting disease harms sea urchins to the extent of mass mortality in the ocean and echinocultures, accompanied by environmental damage and economic losses. The current study emphasizes the antimicrobial resistance of three isolated bacteria, closely related to Vibrio harveyi, Vibrio owensii, and Vibrio fortis, associated with red spotting in the cultured sea urchin Tripneustes gratilla. In vitro trials examined the susceptibility of these bacterial isolates to various antibiotics. In addition, using an in silico examination, we revealed the arsenal of antimicrobial resistance genes in available genomes of various pathogenic Vibrio associated with diseases in sea urchins, fish, shellfish, and corals. These two approaches enabled the discussion of the similarities and differences between aquatic pathogenic Vibrio and their antibiotic resistance. Among them, we revealed a core resistance to tetracyclines and penams by the in vitro examined strains. At the same time, the in silico study also supported this core resistance by the presence of the adeF and CRP genes in the bacterial genomes. Nevertheless, variability and specific resistance were evident at the species and strain levels in the Vibrio bacteria and genomes. The in vitro trials highlighted the diverse resistance of the Vibrio harveyi-like isolate to all examined antibiotics, while the other two isolates were found susceptible to nitrofurantoin and sulfamethoxazole. The resistance of the Vibrio harveyi-like isolate could not have been obtained in the genome of the proposed relative of Vibrio harveyi VHJR7 that lacks the oqxA and oqxB genes, which enables such a resistance. A unique sensitivity of the Vibrio fortis-like isolate to erythromycin is proposed when compared to other isolated Vibrio and Vibrio genomes that seem capable of resisting this drug. According to the results, we propose nitrofurantoin or sulfamethoxazole for treating two of the red-spotting-associated isolates (Vibrio fortis and Vibrio owensii-like), but not Vibrio harveyi-like. We assume that a shared resistance to some antibiotics by Vibrios is gained by a horizontal gene transfer while previous exposures of a bacterial strain to a specific drug may induce the development of a unique resistance. Finally, we discuss the novel knowledge on antibiotic resistance in Vibrio from the current research in light of the potential risks when using drugs for disease control in aquaculture.

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Isolation and characterization of Vibrio owensii from Palk Bay and its infection study against post larvae of Litopenaeus vannamei

Cited by 3 publications

References 39 publications

Identification and antibiotic-resistant properties of Vibrio owensii and V. alginolyticus isolated from the Spermonde Islands, Indonesia

Identification and antibiotic-resistant properties of Vibrio owensii and V. alginolyticus isolated from the Spermonde Islands, Indonesia

Effects of a phytobiotic-based additive on the growth, hepatopancreas health, intestinal microbiota, and Vibrio parahaemolyticus resistance of Pacific white shrimp, Litopenaeus vannamei

Antibiotic Resistance in Vibrio Bacteria Associated with Red Spotting Disease in Sea Urchin Tripneustes gratilla (Echinodermata)

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