Isolation and characterization of Vibrio parahaemolyticus bacteriophage vB_VpaS_PG07

Ding, Tongyan; Sun, Huzhi; Pan, Qinxue; Zhao, Feiyang; Zhang, Zhaozuo; Ren, Huiying

doi:10.1016/j.virusres.2020.198080

Cited by 67 publications

(30 citation statements)

References 33 publications

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“…The thermal and pH stabilities of vB_VpS_PG28 were evaluated under the optimal MOI conditions as previously described with some modifications [ 28 , 29 ]. For thermostability test, the isolated phages were incubated at different temperatures (40°C, 50°C, 60°C and 70°C) with the pH condition of 7.4to test whether the phage could tolerate high temperatures, and aliquots (100 μL) were collected at 20, 40, and 60 mins during the incubation, respectively.…”

Section: Methodsmentioning

confidence: 99%

Characterization and complete genome sequence analysis of a newly isolatedphage against Vibrio parahaemolyticus from sick shrimp in Qingdao, China

Tian

et al. 2022

PLoS ONE

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Foodborne diseases have become a serious havoc, where antimicrobial resistance is throwing significant challenges on daily basis. With the increase of drug-resistant bacteria and food-borne infection associated with Vibrio parahaemolyticus, new and effective strategies were needed to control the emergence of vibriosis. Lytic bacteriophages come up as a promising way to resist the pathogenic population in various applications. In this study, a V. parahaemolyticus specific phage vB_VpS_PG28 was isolated from sewage in the seafood market. Results showed vB_VpS_PG28, is strictly a lytic bacteriophage and has a relatively large burst size of 103 plaque-forming units per infected cell. Comparative genomic and bioinformatic analyses proved that vB_VpS_PG28 is a new bacteriophage that had a homologous relation with Vibrio phages of family Siphoviridae, especially with phage VH2_2019, but transmission electron microscopy of vB_VpS_PG28 morphology characterized its morphology is similar to that of Myoviridae family. In silico analysis indicated that the vB_VpS_PG28 genome consists of 82712 bp (48.08% GC content) encoding 114 putative ORFs without tRNA,and any gene associated with resistance or virulence factors has not been found. The bacteriophage in the present study has shown significant outcomes in order to control bacterial growth under in vitro conditions. Thus, we are suggesting a beneficiary agent against foodborne pathogens. Further, to ensure the safe usage of phage oral toxicity testing is recommended.

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Section: Methodsmentioning

confidence: 99%

Characterization and complete genome sequence analysis of a newly isolatedphage against Vibrio parahaemolyticus from sick shrimp in Qingdao, China

Tian

et al. 2022

PLoS ONE

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“…Cyanobacterial cleavage by cyanophages within natural environments is affected by water temperature, pH, salinity, ions, and UV. To examine the tolerance levels of the phage to these conditions, a published method was used with appropriate modifications [20,21]. The stability of the phage under different temperature treatments was analyzed by taking 1 mL of purified phage solution at different temperatures (0, 25, 40, 60, and 80 • C) at both 30 min and 60 min.…”

Section: Phage Stability Under Environmental Stressmentioning

confidence: 99%

A Novel Wide-Range Freshwater Cyanophage MinS1 Infecting the Harmful Cyanobacterium Microcystis aeruginosa

Zhang

Cao

et al. 2022

Viruses

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Microcystis aeruginosa, as one of the major players in algal bloom, produces microcystins, which are strongly hepatotoxic, endangering human health and damaging the ecological environment. Biological control of the overgrowth of Microcystis with cyanophage has been proposed to be a promising solution for algal bloom. In this study, a novel strain of Microcystis cyanophage, MinS1, was isolated. MinS1 contains an icosahedral head approximately 54 nm in diameter and a 260 nm-long non-contractile tail. The phage genome consists of a linear, double-stranded 49,966 bp DNA molecule, which shares very low homology with known phages in the NCBI database (only 1% of the genome showed weak homology with known phages when analyzed by megablast). The phage contains 75 ORFs, of which 23 ORFs were predicted to code for proteins of known function, 39 ORFs were predicted to code for proteins of unknown function, and 13 ORFs showed no similarity to any protein sequences. Transmission electron microscopy and phylogenetic analysis showed that MinS1 belongs to the family Siphoviridae. Various experiments confirmed that the phage could infect several different orders of cyanobacteria, including Chroococcales, Nostocales, Oscillatoriales, Hormogonales, and Synechococcales, indicating that it has a very broad host range. In addition, MinS1 has no known antibiotic tolerance genes, virulence genes, and tRNAs, and it is tolerant to temperature, pH, UV, and salinity, suggesting that MinS1 has good potential for application as a biological control agent against cyanobacterial blooms. This study expands the diversity and knowledge of cyanophages, and it provides useful information for the development of novel prevention and control measures against cyanobacterial blooms.

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“…The genome sequences were annotated by RAST ( https://rast.nmpdr.org ) ( 7 ) and then checked by BLASTp with the NCBI database ( https://www.ncbi.nlm.nih.gov ) ( 8 ). Phage BUCT86 had 57 open reading frames (ORFs), of which only 25 had predicted functions; the rest were annotated as hypothetical proteins ( Table 1 ).…”

Section: Announcementmentioning

confidence: 99%

Genomic Analysis of Bacteriophage BUCT86 Infecting Klebsiella Pneumoniae

Han

Zhu

et al. 2022

Microbiol Resour Announc

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Isolation and characterization of Vibrio parahaemolyticus bacteriophage vB_VpaS_PG07

Cited by 67 publications

References 33 publications

Characterization and complete genome sequence analysis of a newly isolatedphage against Vibrio parahaemolyticus from sick shrimp in Qingdao, China

Characterization and complete genome sequence analysis of a newly isolatedphage against Vibrio parahaemolyticus from sick shrimp in Qingdao, China

A Novel Wide-Range Freshwater Cyanophage MinS1 Infecting the Harmful Cyanobacterium Microcystis aeruginosa

Genomic Analysis of Bacteriophage BUCT86 Infecting Klebsiella Pneumoniae

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