Isolation and Characterization of Watermelon Isolate of Cucumber green mottle mosaic virus(CGMMV-HY1) from Watermelon Plants with Severe Mottle Mosaic Symptoms

Shim, Chang Ki; Han, Ki Soo; Lee, Jung Han; Bae, Dong Won; Kim, Dong Kil; Kim, Hee Kyu

doi:10.5423/ppj.2005.21.2.167

Cited by 26 publications

(17 citation statements)

References 6 publications

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“…The tobamovirus, CGMMV causes severe disease of cucurbits worldwide. The typical symptoms in watermelon including leaf mottling and mosaic have been reported in greenhouse-grown plants (Varveri et al, 2002;Shim et al, 2005), but these symptoms are masked in plants growing in open fields (Reingold et al, 2013). Serological testing by ELISA of a large number of fruits exhibiting systemic mosaic and mild to severe yellow mottling symptoms, confirmed the presence of CGMMV.…”

Section: Discussionmentioning

confidence: 76%

“…Among those detection methods, RT-PCR (Ugaki et al, 1991;Shim et al, 2005;Yoon et al, 2008;Liu et al, 2009), real time RT-PCR (Chen et al, 2008, transmission electron microcopy (Alshahwan and Abdalla, 1992), immune capture-RTPCR (Celix et al, 1996), ELISA (Antignus et al, 1990;Celix et al, 1996;Mitsuhiro et al, 2006) and monoclonal antibodies (Antignus et al, 2001). To establish procedure of identification and detection, mechanical inoculation, DAS-ELISA, RT-PCR, molecular hybridization and nucleotide sequence were applied in this study.…”

Section: Discussionmentioning

confidence: 99%

“…Thereafter reports were made in USA and Canada (Tian et al, 2014;Ling et al, 2014); Saudi Arabia (Al-Shahwan and Abdalla, 1992; Al-Saleh and Al- Shahwan, 1997); Iran (Moradi and Jafarpour, 2011), China and Greece (Zhang et al, 2009;Varveri et al, 2002), Korea and Myammar (Yoon et al, 2008;Kim et al, 2010), and in the Ukraine (Budzanivska et al, 2007). Several strains of CGMMV have been reported from Europe, Pakistan, India and Japan (Komuru et al, 1968; Lee, 1996;Antignus et al, 1990;Francki et al, 1986;Ugaki et al, 1991;Shim et al, 2005;Ko et al, 2006). CGMMV is mechanically and seed transmitted with a narrow host range comprising of cucurbits only and is different in host range from Tobacco mosaic virus (TMV) whose main hosts are members of the solanaceae (Antignus et al, 1990(Antignus et al, , 2001.…”

Section: Introductionmentioning

confidence: 99%

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Biological and Molecular Characterization of Cucumber green mottle mosaic virus Affecting Bottle Gourd and Watermelon Plants in Saudi Arabia

Amer¹

2015

IJAB

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To cite this paper: Amer, M.A., 2015. Biological and molecular characterization of cucumber green mottle mosaic virus affecting bottle gourd and watermelon plants in Saudi Arabia. AbstractSamples were collected from seven of each bottle gourd and watermelon plants grown in Riyadh and Hail regions of Saudi Arabia in May, 2012 showing symptom suspected to be virus infection and analyzed by a double antibody sandwich against bottle gourd and watermelon viruses. Two of watermelon and three of bottle gourd samples were found to be positive with Cucumber green mottle mosaic virus (CGMMV). However, variable results were obtained with other viruses infecting cucurbits. One sample of CGMMV positive samples from each region were homogenized in a mortar separately and used to make mechanical inoculation on 25 plant species which produced characteristic symptoms of tobamo virus infection. Five ELISA-positive samples from naturally infection were further tested and detected by RT-PCR assay using virus specific primers that amplified a 400 bp fragment in the coat protein gene. The nucleotide sequence of the PCR products from the Saudi isolates of CGMMV isolated from bottle gourd and watermelon were determined and demonstrated its similarity to the other isolates of CGMMV recorded in NCBI. The CP gene of CGMMV-Saudi Arabia isolates shared between 87.2% and 99.7% identity in nucleotide level. The synthesized cDNA probe for CGMMV detection was hybridized with nucleic acid extracts from infected samples collected from the above mentioned locations. This is the first report regarding the genetic makeup of CGMMV at the molecular level in Saudi Arabia.

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Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biological and Molecular Characterization of Cucumber green mottle mosaic virus Affecting Bottle Gourd and Watermelon Plants in Saudi Arabia

Amer¹

2015

IJAB

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“…Numerous techniques have been established for the detection and diagnosis of CGMMV in cucurbit crops: reverse transcription polymerase chain reactions (RT-PCR) (Ugaki et al, 1991;Shim et al, 2005;Liu et al, 2009), real-time RT-PCR (Chen et al, 2008), transmission electron microcopy (TEM) (Antignus et al, 1990, Ibrahim andOmer, 1992), immunocapture (IC)-RT-PCR (Celix et al, 1996), dot blot immunobinding assay (DBIA) , enzyme-linked immunosorbent assays (ELISA) using polyclonal antibodies (PAbs) (Antignus et al, 1990, Mitsuhiro et al, 2006, direct tissue blot immunoassays (DTBIA) and assays involving monoclonal antibodies (MAbs) (Antignus et al, 2001;Shim et al, 2006). Among those detection methods, ELISA, DBIA and DTBIA are most suitable for routine detection in large-scale samples in field surveys; whereas the more sensitive IC-RT-PCR is well-suited for studies of the viral genome .…”

Section: Introductionmentioning

confidence: 99%

Development of a fluorescent in situ hybridization (FISH) technique for visualizing CGMMV in plant tissues

Shargil

Zemach

Belausov

et al. 2015

Journal of Virological Methods

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“…CGMMV causes the appearance of mosaic and green or white spots on the leaves and fruit as well as damage to the quality and quantity of fruit. Th e watermelon strain of CGMMV causes severe disease symptoms in plants, particularly the decline of fruit which results in economic losses to watermelon growers (Shim et al 2005). CYSDV is a destructive cucurbit virus in large areas of the world that reduces the yield from 30 to 50 percent (Louro et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Identification of viruses infecting cucurbits and determination of genetic diversity of Cucumber mosaic virus in Lorestan province, Iran

Hasanvand

Shams-Bakhsh

2017

Journal of Plant Protection Research

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Various viral pathogens infect Cucurbitaceae and cause economic losses. Th e aim of the present study was to detect plant viral pathogens including Cucumber mosaic virus (CMV), Cucumber green mottle mosaic virus (CGMMV), Zucchini yellow mosaic virus (ZYMV), Cucurbit yellow stunting disorder virus (CYSDV) and Cucurbit chlorotic yellows virus (CCYV) in Lorestan province, in western Iran, and also to determine CMV genetic diversity in Iranian populations. A total of 569 symptomatic leaf samples were collected in 2013 and 2014 from cucurbits growing regions in Lorestan province. Th e collected samples were assessed for viral diseases by ELISA. Th e results showed virus incidences in most regions. Th en, the infection of 40 samples to CMV was confi rmed by RT-PCR. Moreover, to distinguish between the two groups (I and II) of CMV, PCR products were digested by two restriction enzymes XhoI and EcoRI. Results of the digestion showed that the isolates of Lorestan belonged to group I. Th e CMV-coat protein gene of eight isolates from diff erent regions and hosts was sequenced and phylogenetic analysis was performed. Subsequent analyses showed even more genetic variation among Lorestan isolates. Th e phylogenetic tree revealed that Lorestan province isolates belonged to two IA and IB subgroups and could be classifi ed together with East Azerbaijan province isolates. Th e results of the present study indicate a wide distribution of CMV, ZYMV, CGMMV, CYSDV and CCYV viruses in cucurbits fi elds of Lorestan province and for the fi rst time subgroup IB of CMV was reported on melon from Iran.

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Isolation and Characterization of Watermelon Isolate of Cucumber green mottle mosaic virus(CGMMV-HY1) from Watermelon Plants with Severe Mottle Mosaic Symptoms

Cited by 26 publications

References 6 publications

Biological and Molecular Characterization of Cucumber green mottle mosaic virus Affecting Bottle Gourd and Watermelon Plants in Saudi Arabia

Biological and Molecular Characterization of Cucumber green mottle mosaic virus Affecting Bottle Gourd and Watermelon Plants in Saudi Arabia

Development of a fluorescent in situ hybridization (FISH) technique for visualizing CGMMV in plant tissues

Identification of viruses infecting cucurbits and determination of genetic diversity of Cucumber mosaic virus in Lorestan province, Iran

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