Isolation and Characterization of Wound Healing Compounds from Chloroform Extract of Binahong Leaves (Anredera cordifolia (Ten.) Steenis)

Abstract

The leaves of Binahong (Anredera cordifolia (Ten) Steenis) have been widely studied to have benefits and uses in various medical purposes, one of which is in the wound healing process. The existence of several active compounds contained in the leaves of the Binahong tree plant have an important role in wound healing, such as flavonoids, terpenoids, alkaloids, saponins, ascorbic acid and citrate compounds, these compounds are the targeted for further process of this natural product. The eminence of the extraction method that is used repeatedly and consistently to obtain pure compounds from Binahong leaves to be isolated is the one of topics discussed in this literature study. The perspective of implementation from liquid-solid extraction followed by liquid-liquid extraction using chloroform as a solvent is discussed comprehensively in this article. The identification of the isolation of compounds are able to conducted by implemented a thin layer chromatography (TLC) techniques, and for the further structural determination by UV, MS, FTIR, NMR spectroscopy and separation techniques using GC-MS. Respectively, the components are successfully to be identified and reported in previous research, for the following criteria that have been completed, some isolated active compound are purposed as the candidate for the wound healing agent. For the further application it is reasonable to implied these matters for some pharmaceutical products.

Acute toxicity evaluation of ethanolic extract of the leaves of Anredera cordifolia in wistar rats (Rattus Norvegicus)

“…A study revealed the influence of binahong leaf extract on analgesic activity in male mice infused at a dose of 400 mg/Kg BW [12]. The compounds in binahong leaves that might implicate to act as analgesics are flavonoid glycosides, saponins, vitamin C, and flavones [13]. The mechanism of action of flavonoids as analgesics is by inhibiting the enzyme cyclooxygenase (COOX), thereby reducing the production of prostaglandins by arachidonic acid which causes pain.…”

Diabetic Wound Healing Activity of Anabas testudineus Fish Scales

“…17 There are four wound healing phases: the coagulation phase, the inflammatory phase, the proliferation/migration/reepithelialization/ granulation phase, and the remodeling/maturation phase (figure 4). 18,19 In patients with diabetes mellitus, nonhealing/chronic wounds occur; in this condition, the inflammatory phase is prolonged. Diabetic wounds exhibit a persistent inflammatory phase associated with an impediment in forming mature granulation tissue and a reduction in wound tensile strength.…”

Utilization of Nanogels Extract Leaf Binahong on Palatal Wound Healing

“…Saponins also stimulate fibroblast proliferation and collagen formation. 26,27 Tannins and alkaloids are antimicrobial and antioxidant, which can prevent and protect the wound area from being damaged by free radicals and inhibit the growth of pathogenic bacteria in the wound.…”

“…Binahong leaf alkaloids are antibacterial by interfering with the peptidoglycan component of bacterial cells so that the cell wall layer is not completely formed and cell death occurs. 26,27 Ascorbic acid (vitamin C) functions to increase resistance to infection, maintain mucous membranes, and accelerate wound healing. 28 The use of nanogels in mucosal wound healing shows that CTL nanogel has a significant effect on cell proliferation at various concentrations, its use as a safe and effective drug delivery system.…”