2020
DOI: 10.21203/rs.3.rs-20498/v2
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Isolation and Characterization of WUPyV in Polarized Human Airway Epithelial Cells

Abstract: Abstract Background: Washington University polyomavirus (WUPyV) is a novel human polyomavirus detected in childwith acute respiratory infection in 2007. However, the relationship between WUPyV and respiratory diseases has yet to be established for lacking of a suitable in vitro culture system. Methods: To isolate WUPyV with human airway epithelial (HAE) cells, the positive samples were incubated in HAE, and then the nucleic acid, VP1 protein and virions were detected us… Show more

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