Isolation and cloning of an endo-β-1,4-mannanase from Pacific abalone Haliotis discus hannai

An endo-β-1,4-mannanase, AkMan, from the common sea hare Aplysia kurodai

“…edulis (39,216 and 39,265 Da) (Xu et al, 2002a) and H. discus hannai (39,627 Da) (Ootsuka et al, 2006). The N-terminal and partial amino-acid sequences of AkMan showed high similarity to those of the above molluscan enzymes belonging to GHF5.…”

“…MSJ-5 (Zhang et al, 2009). Complete inactivation by Ag + and 40-50% inactivation by Co 2+ , Fe 2+ and Cu 2+ were reported in Haliotis, Pomacea and Littorina enzymes (Ootsuka et al, 2006;Yamaura and Matsumoto, 1993;Yamaura et al, 1996). Mannanases from Streptomyces sp., Pseudomonas sp.…”

“…-1,4-Mannanase has been isolated from bacteria (Araki, 1983 ;Akino et al, 1988;Talbot et al, 1990;Braithwaite et al, 1995;Nakajima and Matsuura, 1997;Li et al, 2000;Politz et al, 2000;Kansoh and Nagieb, 2004;Li et al, 2006), fungi (Johnson, 1990;Stalbrand et al, 1993;Kurakake et al, 2001;Puchart et al, 2004;Naganagouda et al, 2009), higher plants (Shimahara et al, 1975;Marraccini et al, 2001), and mollusks (Yamaura and Matsumoto, 1993;Yamaura et al, 1996;Xu et al, 2002a andOotsuka et al, 2006). Compared with bacterial and fungal enzymes, molluscan enzymes have not been well investigated, i.e., only four -1,4-mannanases have been investigated in Pomacea insularus (Yamaura and Matsumoto, 1993), Littorina brevicula (Yamaura et al, 1996), Mytilus edulis (Xu et al, 2002a and b), and Haliotis discus hannai (Ootsuka et.…”

“…Compared with bacterial and fungal enzymes, molluscan enzymes have not been well investigated, i.e., only four -1,4-mannanases have been investigated in Pomacea insularus (Yamaura and Matsumoto, 1993), Littorina brevicula (Yamaura et al, 1996), Mytilus edulis (Xu et al, 2002a and b), and Haliotis discus hannai (Ootsuka et. al., 2006).…”

Enzymatic properties and primary structures of two α-amylase isozymes from the Pacific abalone Haliotis discus hannai

“…The feeding behavior of abalone has long been investigated from the view point of aquaculture (Mai et al, 1995;Takami et al, 1998) and enzymatic properties of polysaccharide-degrading enzymes of abalone have also been investigated to enrich the knowledge for the digestion mechanisms of seaweeds' polysaccharides by abalone (Shimizu et al, 2003;Suzuki et al, 2003;Suzuki et al, 2006;Ootsuka et al, 2006;Kumagai and Ojima 2009). Such information seems to be indispensable to understand how marine mollusks acquire carbohydrate nutrients from seaweeds.…”