Isolation and comparative expression analysis of six MBD genes in wheat

Li, Yongchun; Meng, Fanrong; Yin, Jing; Liu, Haoying; Si, Zhifei; Sun, Qixin; Ren, Jie; Niu, Hongbin

doi:10.1016/j.bbagrm.2007.09.004

Cited by 13 publications

(12 citation statements)

References 35 publications

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“…The UBIQUITIN endogenous control gene (accession no. AY297059) has been described previously (Li et al, 2008). Wheat Rht-D1 Mercia NILs were used to determine Rht-D1 gene dosage in an Rht-D1 allelic series.…”

Section: Real-time Qrt-pcrmentioning

confidence: 99%

Molecular Characterization ofRht-1Dwarfing Genes in Hexaploid Wheat

et al. 2011

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The introduction of the Reduced height (Rht)-B1b and Rht-D1b semidwarfing genes led to impressive increases in wheat (Triticum aestivum) yields during the Green Revolution. The reduction in stem elongation in varieties containing these alleles is caused by a limited response to the phytohormone gibberellin (GA), resulting in improved resistance to stem lodging and yield benefits through an increase in grain number. Rht-B1 and Rht-D1 encode DELLA proteins, which act to repress GA-responsive growth, and their mutant alleles Rht-B1b and Rht-D1b are thought to confer dwarfism by producing more active forms of these growth repressors. While no semidwarfing alleles of Rht-A1 have been identified, we show that this gene is expressed at comparable levels to the other homeologs and represents a potential target for producing novel dwarfing alleles. In this study, we have characterized additional dwarfing mutations in Rht-B1 and Rht-D1. We show that the severe dwarfism conferred by Rht-B1c is caused by an intragenic insertion, which results in an in-frame 90-bp insertion in the transcript and a predicted 30-amino acid insertion within the highly conserved amino-terminal DELLA domain. In contrast, the extreme dwarfism of Rht-D1c is due to overexpression of the semidwarfing Rht-D1b allele, caused by an increase in gene copy number. We show also that the semidwarfing alleles Rht-B1d and Rht-B1e introduce premature stop codons within the amino-terminal coding region. Yeast two-hybrid assays indicate that these newly characterized mutations in Rht-B1 and Rht-D1 confer "GA-insensitive" dwarfism by producing DELLA proteins that do not bind the GA receptor GA INSENSITIVE DWARF1, potentially compromising their targeted degradation.

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Section: Real-time Qrt-pcrmentioning

confidence: 99%

Molecular Characterization ofRht-1Dwarfing Genes in Hexaploid Wheat

et al. 2011

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“…Commonly, more bands were expressed in one parent and the F 1 , but not in the other parent, whereas less bands were expressed only in the F 1 and not in either parent [21][22][23][24][25]. Also, sometimes the bands expressed only in the F 1 significantly correlated to some heterosis, and the bands expressed in one parent and the F 1 but not in the other parent did not correlate to total heterosis [27][28][29][30][31][32]. Some heterosis was related to specific gene expression, although this accounted for less among total expressed genes.…”

Section: Discussion Of the Heterosis Theory Based On Qtgmentioning

confidence: 90%

“…Many studies approved the conservativeness of constitutive genes [21,23,26,27] and the universality of trait differences due to differential gene expression [29][30][31][32][33][34][35][36][37][38]. Some authors point out the strict conservativeness, the great stability of gene expression, and the adjustable properties of their expression [21][22][23][24][25][26][27][28][29][30][31][32]. Furthermore, based on the constitutive gene expression, they considered that heterosis was derived from expression to different degrees, from random or non-random [40], and non-equivalent [41] expression.…”

Section: Discussion Of the Heterosis Theory Based On Qtgmentioning

confidence: 99%

“…The foundation level of heterosis may be controlled by the hereditable additive and additive epistasis of pure DNA factors. However, the number of pure DNA factors engaged in the basic expression of heterosis are few [2, [21][22][23][24][25][26][27][28]. In each growth stage, and in each organ and tissue of cotton plants, genes are differently expressed [29][30][31][32][33][34][35][36][37][38], based on the indirect evidence of differential gene expression.…”

Section: Discussion Of the Heterosis Theory Based On Qtgmentioning

confidence: 99%

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Primary analysis of QTG contribution to heterosis in upland cotton

et al. 2010

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In this paper, we analyzed the contribution of pure DNA factors to heterosis using quantitative trait genes (QTG) in two randomly selected strains from a recombinant inbred line of Gossypium hirsutum. According to a set of QTL mapping results, combined with analysis of DNA recombinant fragment sources in the two strains and QTL association analysis with their field traits, we hypothesize a view of "dominance + overdominance + epistasis". That is, additive and additive epistasis may be the genetic basis of heterosis, and dominance, overdominance and epistasis may be the modes of heterosis action. Based on the heterosis results of this study, we also suggest a molecular mechanism for heterosis, and explain, in detail, with two randomly selected strains as examples. The male and female parent-derived additive epistatic QTLs of upper half mean length in LG01 and LG03 produced a trait variance of 2.99-3.52 compared with the female parent-derived loci. The trait of bolls per plant was controlled by two pairs of additive epistatic QTLs in LG02 and LG07, which were derived from both female and male parents. The QTLs were reciprocally interacted and produced a trait variance of 0.86. An initial concept of "super-hybrid cotton" was raised according to the nature of additive effect, that is genetic stability. upland cotton, recombinant inbred line, quantitative trait gene, heterosis, additive epistasis Citation: Zhang X L, Liu F, Wang W, et al. Primary analysis of QTG contribution to heterosis in upland cotton.

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“…Previously, we isolated six wheat cDNAs with open reading frame encoding putative methyl-binding domain proteins, designated TaMBD1 to TaMBD6, respectively (Li et al, 2008). In this study, the genomic and cDNA sequences of three TaMBD2 homoeologous genes (TaMBD2-5A, TaMBD2-5B and TaMBD2-5D) were obtained, and their spatial expression patterns were analyzed.…”

Section: Introductionmentioning

confidence: 99%

Expression divergence of TaMBD2 homoeologous genes encoding methyl CpG-binding domain proteins in wheat (Triticum aestivum L.)

Wang

et al. 2011

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Isolation and comparative expression analysis of six MBD genes in wheat

Cited by 13 publications

References 35 publications

Molecular Characterization ofRht-1Dwarfing Genes in Hexaploid Wheat

Molecular Characterization ofRht-1Dwarfing Genes in Hexaploid Wheat

Primary analysis of QTG contribution to heterosis in upland cotton

Expression divergence of TaMBD2 homoeologous genes encoding methyl CpG-binding domain proteins in wheat (Triticum aestivum L.)

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