Isolation and cross-species characterization of polymorphic microsatellites for the orchid bee Eulaema meriana (Hymenoptera: Apidae: Euglossini)

Abstract

We describe and characterize eight polymorphic microsatellite loci for the orchid bee species Eulaema meriana, an abundant species and important pollinator in wet lowland forests in tropical America. We also tested the cross-species amplification of these microsatellite loci in seven other species of the genus Eulaema. For E. meriana, number of alleles per locus ranged from four to nine and expected heterozygosity ranged from 0.377 to 0.854. Seven out of the eight loci described amplified in all seven other Eulaema species. These microsatellite loci will be of practical use for population structure, mating system and inbreeding studies in euglossine bees.

Climate, physiological tolerance and sex‐biased dispersal shape genetic structure of Neotropical orchid bees

“…All mitochondrial and nuclear coding genes were sequenced in both directions, while the ASCN loci were sequenced only in the forward strand using a 24‐bp tag added to the forward primers (López‐Uribe et al . ). Fragments were sequenced using the Big Dye terminator kit (version 3.1) (Applied Biosystems) on an ABI 3730 capillary sequencer.…”

Identification of 37 microsatellite loci for Anthophora plumipes (Hymenoptera: Apidae) using next generation sequencing and their utility in related species

“…One of the reasons for this is a general lack of genetic markers for non-model organisms. Although the microsatellite loci of stingless bees are well studied (e.g., Francini et al, 2010;Francisco et al, 2011;Pereira et al, 2011) and there is a lot of literature on microsatellites in bumblebees (Estoup et al, 1995Funk et al, 2006, Stolle et al, 2009, microsatellites have only been developed for 10 species of solitary bees (Paxton et al, 1996(Paxton et al, , 2003Mohra et al, 2000;Kukuk et al, 2002;Azuma et al, 2005;Zayed, 2006;Souza et al, 2007;Soro & Paxton, 2009;Lopez-Uribe, 2011) and as a consequence there are few studies on the population structure and other biological aspects of these bees.…”

Determinação da variabilidade genético-morfológica em populações de <i>Euglossa pleosticta</i> Dressler, 1982 (Apidae, Euglossini)

“…Desta forma, diversos microssatélites foram descritos para diferentes grupos de abelhas, buscando assim um melhor entendimento e bom resultado da genotipagem, levando em consideração a necessidade do uso de marcadores espécie-específico (Estoup et al, 1995;Souza et al, 2007;Brito et al, 2009;Paxton et al, 2009;Augusto et al 2011;López-Uribe et al, 2011;Francisco et al, 2011). Em estudos populacionais envolvendo as abelhas Euglossini, fazendo uso de microssatélites especificamente desenvolvidos para a tribo, foram comuns os relatos de uma alta diversidade genética (Cerantôla et al, 2010;Souza et al, 2010;Freiria et al, 2012.…”