2023
DOI: 10.3389/fvets.2023.1116671
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Isolation and cultivation as well as in situ identification of MSCs from equine dental pulp and periodontal ligament

Abstract: IntroductionThe lifelong eruption places a great demand on the dental pulp and periodontal ligament (PDL) of horse teeth. Cells within the pulp and PDL seem to play a key role during this remodeling.MethodsIn this study, we isolated and cultivated MSCs (medicinal signaling cells) from dental pulp, PDL and retrobulbar fat of four horses. Subsequently, we analyzed them by flow cytometry and immunohistochemistry to determine and compare their characteristics. In addition, we localized these cells within the tissu… Show more

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