1985
DOI: 10.2307/3281546
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Isolation and Cultivation of Plasmodium falciparum Using Adult Bovine Serum

Abstract: RPMI 1640 medium supplemented with adult bovine serum and hypoxanthine was superior to human serum-supplemented medium for the isolation of new strains of Plasmodium falciparum in Sudan. Similar observations in Indonesia have since confirmed our results. The chloroquine sensitivity of new isolates was identical in either human or bovine serum. Once acclimated to culture conditions P. falciparum strains grew better when using human serum. Erythrocyte-specific antibody present in adult bovine serum slightly inhi… Show more

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Cited by 16 publications
(13 citation statements)
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“…The purines supplied with erythrocytes, however, can be consumed as rapidly as a high proportion of the erythrocytes becoming infected, as noted in our observations. Several methods have been described for the cultivation of P. falciparum using bovine serum (Divo et al 1985), exhaustively dialysed human serum (Divo & Jensen, 1982), human highdensity lipoprotein fraction (Grellier et al 1991), and commercial Nutridoma-SR (Lingnau et al 1993), and their success may be explained by the presence of hypoxanthine ranging from 30 to 375 JIM in the culture media, although Ofulla et al (1993) described that RPMI 1640 supplemented with bovine serum albumin sustained growth of the parasites without added hypoxanthine. On the other hand, the level of hypoxanthine in human plasma obtained from venous blood has been shown to be 1-8-2-5 fiM (Kugler, 1978), equally 018-0-25 /*M in a culture medium with 10% human serum.…”
Section: -Day Cultures Are Shown Inmentioning
confidence: 99%
“…The purines supplied with erythrocytes, however, can be consumed as rapidly as a high proportion of the erythrocytes becoming infected, as noted in our observations. Several methods have been described for the cultivation of P. falciparum using bovine serum (Divo et al 1985), exhaustively dialysed human serum (Divo & Jensen, 1982), human highdensity lipoprotein fraction (Grellier et al 1991), and commercial Nutridoma-SR (Lingnau et al 1993), and their success may be explained by the presence of hypoxanthine ranging from 30 to 375 JIM in the culture media, although Ofulla et al (1993) described that RPMI 1640 supplemented with bovine serum albumin sustained growth of the parasites without added hypoxanthine. On the other hand, the level of hypoxanthine in human plasma obtained from venous blood has been shown to be 1-8-2-5 fiM (Kugler, 1978), equally 018-0-25 /*M in a culture medium with 10% human serum.…”
Section: -Day Cultures Are Shown Inmentioning
confidence: 99%
“…Some media contain more vitamins and other components, such as cholesterol, fatty acids, purines, and pyrimidines, compared with RPMI 1640. The following are some commercially available media that support the in vitro growth of asexual intraerythrocytic stages of P. falciparum: M-199 with either Hank's or Eagle's salt solution, Ham's nutrient mixture F-12, DMEM, IMDM, Waymouth medium, and NCTC 135 (medium formulated at the National Cancer Institute, Tissue Culture section) [26,30,[92][93][94][95][96][97][98][99]. In addition to these media, RPMI 1630 was reported to support parasite growth in vitro [96], but it is not available from commercial sources.…”
Section: Alternative Culture Mediamentioning
confidence: 99%
“…In another study [ 30 ], M-199 was supplemented with adenosine triphosphate (ATP), adenosine, inositol, coenzyme A, creatinine, glutathione, glyglycine, d -glucose, vitamin C, 15–20% pooled, and heat-inactivated calf serum to initiate the long-term cultivation of cryopreserved isolates and maintain the strain for over a year. The growth of a laboratory-adapted strain routinely maintained in RPMI 1640 was compared in Ham’s F12, M-199 with Earle’s salts, and M-199 with Hank’s salts after a 96 h incubation [ 93 ]. Parasite growth in Ham’s F12 was comparable, or slightly less, with RPMI 1640 when the media were supplemented with 5% or 10% human serum.…”
Section: Culture Media and Nutritional Requirementsmentioning
confidence: 99%
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