Isolation and cultivation of spore-forming filamentous bacteria from<i>Porcellio scaber</i>

Jorgensen, Jeremy; Dolan, Sona; Haselton, Aaron; Kolchinsky, Rita

doi:10.1139/m97-017

Cited by 9 publications

(9 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Because strains of B. cereus and B. anthracis are indistinguishable by 16S rDNA sequence analysis (27), identification is based on physiology. Phenotypic data confirmed arthromitid JJ#1 as a strain of B. cereus (18).…”

Section: Resultsmentioning

confidence: 57%

“…were conspicuous components of the intestinal community in all arthropods studied here except Porcellio scaber. Although long attached filaments were noted in dark-reared sow bugs, short filaments, uni-and diplocells were always more abundant in the typically dry intestines of sow bugs (18).…”

Section: Resultsmentioning

confidence: 82%

“…The extent of filament formation and the tendency to be motile decline as cultures mature and sporulate. Conditions favoring sporulation and retention of filament morphology (up to 180 cell lengths) were discerned for the sow bug strain JJ#1 (18). Sporulation was accelerated by light incubation but filaments were longer and more abundant in dark-incubated cultures of sow bug Arthromitus ( Table 2).…”

Section: Resultsmentioning

confidence: 96%

“…Y15466) (18) showed it to belong to the B. cereus͞B. anthracis 16S rDNA phylogenetic cluster when compared with B. cereus sequences (accession nos.…”

Section: Resultsmentioning

confidence: 99%

“…Relative frequency of morphotype apparently depends on environmental contingencies. 18). Several hundred animals were maintained on damp soil, wood chips, and dry leaves in plastic boxes.…”

Section: Resultsmentioning

confidence: 99%

See 4 more Smart Citations

The Arthromitus stage of Bacillus cereus : Intestinal symbionts of animals

Margulis

Jorgensen²,

Dolan³

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

131

View full text Add to dashboard Cite

In the guts of more than 25 species of arthropods we observed filaments containing refractile inclusions previously discovered and named ''Arthromitus'' in 1849 by Joseph Leidy [Leidy, J. (1849) Proc. Acad. Nat. Sci. Philadelphia 4, 225-233]. We cultivated these microbes from boiled intestines of 10 different species of surface-cleaned soil insects and isopod crustaceans. Literature review and these observations lead us to conclude that Arthromitus are spore-forming, variably motile, cultivable bacilli. As long rod-shaped bacteria, they lose their flagella, attach by fibers or fuzz to the intestinal epithelium, grow filamentously, and sporulate from their distal ends. When these organisms are incubated in culture, their life history stages are accelerated by light and inhibited by anoxia. Characterization of new Arthromitus isolates from digestive tracts of common sow bugs (Porcellio scaber), roaches (Gromphodorhina portentosa, Blaberus giganteus) and termites (Cryptotermes brevis, Kalotermes flavicollis) identifies these flagellated, spore-forming symbionts as a Bacillus sp. Complete sequencing of the 16S rRNA gene from four isolates (two sow bug, one hissing roach, one death's head roach) confirms these as the low-G؉C Gram-positive eubacterium Bacillus cereus. We suggest that B. cereus and its close relatives, easily isolated from soil and grown on nutrient agar, enjoy filamentous growth in moist nutrient-rich intestines of healthy arthropods and similar habitats.

show abstract

Section: Resultsmentioning

confidence: 57%

Section: Resultsmentioning

confidence: 82%

Section: Resultsmentioning

confidence: 96%

“…Y15466) (18) showed it to belong to the B. cereus͞B. anthracis 16S rDNA phylogenetic cluster when compared with B. cereus sequences (accession nos.…”

Section: Resultsmentioning

confidence: 99%

“…Relative frequency of morphotype apparently depends on environmental contingencies. 18). Several hundred animals were maintained on damp soil, wood chips, and dry leaves in plastic boxes.…”

Section: Resultsmentioning

confidence: 99%

See 3 more Smart Citations

The Arthromitus stage of Bacillus cereus : Intestinal symbionts of animals

Margulis

Jorgensen²,

Dolan³

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

131

View full text Add to dashboard Cite

show abstract

Bacillus

Logan¹,

Vos²

2015

Bergey's Manual of Systematics of Archaea and Bacteria

101

View full text Add to dashboard Cite

Ba.cil'lus. N.L. masc. n. Bacillus a rodlet. Firmicutes / “Bacilli” / Bacillales / Bacillaceae / Bacillus Cells rod‐shaped, straight or slightly curved, occurring singly and in pairs, some in chains, and occasionally as long filaments. Endospores are formed, no more than one to a cell; these spores are very resistant to many adverse conditions. Gram‐positive, or Gram‐positive only in early stages of growth, or Gram‐negative. A meso ‐DAP direct murein cross‐linkage type is commonest, but L ‐Lys‐ D ‐Glu, Orn‐ D ‐Glu and L ‐Orn‐ D ‐Asp have occasionally been reported. Motile by means of peritrichous or degenerately peritrichous flagella, or nonmotile. Aerobes or facultative anaerobes, but a few species are described as strictly anaerobic. The terminal electron acceptor is oxygen, replaceable by alternatives in some species. Most species will grow on routine media such as nutrient agar and blood agar. Colony morphology and size very variable between and within species. A wide diversity of physiological abilities is exhibited, ranging from psychrophilic to thermophilic, and acidophilic to alkaliphilic; some strains are salt tolerant and some are halophilic. Catalase is produced by most species. Oxidase‐positive or ‐negative. Chemo‐organotrophic; two species are facultative chemolithotrophs: prototrophs to auxotrophs requiring several growth factors. Mostly isolated from soil, or from environments that may have been contaminated directly or indirectly by soil, but also found in water, food and clinical specimens. The resistance of the spores to heat, radiation, disinfectants, and desiccation results in species being troublesome contaminants in operating rooms, on surgical dressings, in pharmaceutical products and in foods. Most species have little or no pathogenic potential and are rarely associated with disease in humans or other animals; an exception is Bacillus anthracis , the agent of anthrax; several other species may cause food poisoning and opportunistic infections, and strains of Bacillus thuringiensis are pathogenic to invertebrates. DNA G + C content (mol%) : 32–66 ( T m ). Type species : Bacillus subtilis Cohn 1872, 174 AL .

show abstract