Isolation and culture of human endometrial cells in a three-dimensional culture system

Bentin-Ley, Ursula; Pedersen, Bent; Lindenberg, Svend; Larsen, Julie Brogaard; Hamberger, Lars; Horn, Thomas

doi:10.1530/jrf.0.1010327

Cited by 113 publications

(58 citation statements)

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“…A few studies have used in vitro models of human implantation that focused mainly on ultrastructural relationships between the attached blastocyst and the endometrial epithelium, e.g. in a three dimensional cell culture system [81][82][83][84]. Carver et al [85] demonstrated hCG-secreting activity of a blastocyst implanted into endometrial stromal cells monolayer.…”

Section: Model Of Early Embryonic Implantationmentioning

confidence: 99%

Uterine receptivity to human embryonic implantation: Histology, biomarkers, and transcriptomics

Aghajanova¹,

Hamilton²,

Giudice³

2008

Seminars in Cell & Developmental Biology

140

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Embryonic implantation is a dynamic process of paracrine interactions between the maternal compartment and the conceptus and involves a receptive endometrium and a developmentally competent blastocyst. Herein, we review histology, clinical approaches, and the promise of transcriptomics in elucidating mechanisms underlying implantation and development of biomarkers of uterine receptivity -with an eye to diagnose and treat implantation-based disorders of miscarriage, fetal growth restriction, pre-eclampsia, and infertility.Keywords human implantation; endometrium; microarray; blastocyst Challenges and approaches in studying the process of human implantation Clinical implicationsSuccessful embryo implantation is a crucial event in natural and assisted human reproduction. Blastocyst implantation is a dynamic process, involving embryo apposition, attachment to the maternal endometrial epithelium, and invasion into the endometrial stroma [1]. With in vitro fertilization (IVF), implantation failure can occur due to several factors [2], including embryonic defects such as chromosomal abnormalities, which are the most common cause of implantation failure [3,4]. Another widely acknowledged barrier to successful blastocyst implantation and, therefore, successful treatment of infertile women with implantation failure, is an inappropriately developed endometrium. It is well established that embryos cannot implant in a poorly matured endometrium [5], and this may be responsible for low implantation rates with transfer of "good quality" embryos. The success of embryonic implantation further relies upon cross-talk between the embryo and a receptive endometrium [5]. Optimization of IVF results are important for many reasons, and achieving optimal implantation is even more important in the setting of single embryo transfer, a common practice aimed to avoid multiple pregnancies.Corresponding author: Linda C. Giudice, MD, PhD, MSc, Professor and Chair, Department of Obstetrics, Gynecology and Reproductive Sciences, The Robert B. Jaffe, MD Endowed Professor in the Reproductive Sciences, University of California, San Francisco, 505 Parnassus Ave., M1496, Box 0132, San Francisco, CA 94143-0132, Tel: +1 4154762564, Fax: +1 415476181, giudice@obgyn.ucsf.edu. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. The implantation window and challenges of studying it NIH Public AccessThe endometrium is receptive to blastocyst implantation during a spatially and temporally restricted "window", when the luminal epithelium is favorable for blastocyst implantation even in the absence of an implanting ...

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Section: Model Of Early Embryonic Implantationmentioning

confidence: 99%

Uterine receptivity to human embryonic implantation: Histology, biomarkers, and transcriptomics

Aghajanova¹,

Hamilton²,

Giudice³

2008

Seminars in Cell & Developmental Biology

140

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“…Present knowledge relies partly on in vitro models in which embryos, or trophoblastic spheroids derived from cell lines, primary tissue or embryonic stem cells, attach to 2D or 3D cultures of endometrial epithelium from either primary tissue or cell lines (Bentin-Ley et al, 1994;Boggavarapu et al, 2016;Hannan et al, 2009;Kang et al, 2015Kang et al, , 2014Lalitkumar et al, 2013;Lee et al, 2015;Singh and Aplin, 2015;Singh et al, 2010;Weimar et al, 2013). Recent observations of embryos attaching directly to cell-free surfaces demonstrate their self-organising potential (Deglincerti et al, 2016;Shahbazi et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Embryo–epithelium interactions during implantation at a glance

Aplin

Ruane

2017

Journal of Cell Science

199

140

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At implantation, with the acquisition of a receptive phenotype in the uterine epithelium, an initial tenuous attachment of embryonic trophectoderm initiates reorganisation of epithelial polarity to enable stable embryo attachment and the differentiation of invasive trophoblasts. In this Cell Science at a Glance article, we describe cellular and molecular events during the epithelial phase of implantation in rodent, drawing on morphological studies both in vivo and in vitro, and genetic models. Evidence is emerging for a repertoire of transcription factors downstream of the master steroidal regulators estrogen and progesterone that coordinate alterations in epithelial polarity, delivery of signals to the stroma and epithelial cell death or displacement. We discuss what is known of the cell interactions that occur during implantation, before considering specific adhesion molecules. We compare the rodent data with our much more limited knowledge of the human system, where direct mechanistic evidence is hard to obtain. In the accompanying poster, we represent the embryo-epithelium interactions in humans and laboratory rodents, highlighting similarities and differences, as well as depict some of the key cell biological events that enable interstitial implantation to occur.

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“…In addition, in vitro three-dimensional culture systems have been established in which epithelial and stromal cells are co-cultured. The epithelial cells exhibit polarity, with microvilli and pinopodes, and closely resemble endometrium in vivo (Bentin-Ley et al, 1994). These systems offer the potential for studying the local molecular events involved in blastocyst adhesion in vitro, and for elucidating the role played by cytokines in producing an attachment competent luminal epithelium.…”

Section: Summary and Future Prospectsmentioning

confidence: 99%

Cytokines and implantation

Sharkey¹

1998

Reviews of Reproduction

143

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Implantation involves the attachment of the blastocyst to the uterine epithelium. However, in humans and also in domestic animals the failure rate for this process is high. Although the reasons for this are unclear, two factors are necessary for success: the production of a hatched blastocyst capable of implanting and, at the same time, the development of an endometrium that is receptive to the embryo.Overall responsibility for the coordination of these two processes lies with the ovarian hormones, oestrogen and progesterone. However, it is now clear that under their influence, locally acting soluble factors secreted by the endometrium can act on the embryo to influence its development. Developing embryos, in turn, have been shown to produce soluble factors that can act in an autocrine manner, or on the endometrium to influence receptivity (Adamson, 1993; Guidice, 1994). After attachment, embryonic signals also initiate the process of decidualization, by which the endometrium prepares for development of the placenta. Factors involved in the conversation between embryo and endometrium include prostaglandins, leukotrienes, growth factors and cytokines. This article will review new data indicating a critical role for cytokines and growth factors, both in preimplantation embryo development and endometrial receptivity. The aim is to provide an overview of the approaches that have been taken, with a critical appraisal of the methods used and likely future developments. Cytokines and preimplantation embryo developmentAfter fertilization, preimplantation embryos from several species can develop in simple defined medium to produce viable blastocysts (Bowman and McLaren, 1970). However, the growth rate in vitro is slower than in in vivo counterparts, and even short periods of culture in vitro followed by embryo replacement results in developmental delay and reduced implantation rates (Vanderhyden and Armstrong, 1988). In response to these problems, many workers have adopted co-culture systems in which embryos are grown with feeder cell layers, or conditioned medium from them. The effects on embryo development vary greatly depending on the particular combination of medium and cell line used, but good results have been obtained with epithelial cell layers from the uterus or oviduct (Menezo et al., 1990). Similarly, culture of embryos in media conditioned by other embryos or co-culture of multiple embryos together in the same drop improve development (Keefer et al., 1994). These early observations suggested that soluble factors secreted by There is strong evidence that cytokines and growth factors play an important role as local mediators of the actions of steroids on the endometrium to prepare it for implantation. These factors have also been shown to act in both an autocrine and paracrine manner to regulate the development of preimplantation embryos in several species. Attempts to define the function of each cytokine have involved receptor localization, establishment of the mode of control by steroid hormones, and functio...

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Isolation and culture of human endometrial cells in a three-dimensional culture system

Cited by 113 publications

References 7 publications

Uterine receptivity to human embryonic implantation: Histology, biomarkers, and transcriptomics

Uterine receptivity to human embryonic implantation: Histology, biomarkers, and transcriptomics

Embryo–epithelium interactions during implantation at a glance

Cytokines and implantation

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