Isolation and Detection of Alpha Linolenic Acid from Silkworm Pupal Residue Oil (Bombyx mori L.) Using HPLC

Vidhathri, B.S.; Parama, V. R. R.; Subbarayappa, C.T.; Narayanaswamy, T. K.; Muthuraju, R.; Mahesh, D.S.; Vidyashree, D.N.

doi:10.20546/ijcmas.2017.607.258

Cited by 2 publications

(3 citation statements)

References 6 publications

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“…Solvent acetonitrile: methanol: n-hexane in the ratio 90:8:2.Flow rate 2ml/min at 210 nm. The run time was 30 min, and only the B pump was used 12 . First, the standard ALA was loaded and the retention time was observed.…”

Section: Purification Of Alpha-linolenic Acid (Ala)mentioning

confidence: 99%

“…Chromatography techniques are important biophysical techniques for separating and purifying the components of a mixture for qualitative and quantitative studies 9 . Due to their precision and accuracy, one can achieve better results from chromatography in the separation and purification of natural compounds, including oils, when compared to existing separation methods such as molecular distillation, low-temperature crystallization, and urea adduct formation 10,11,12 . Preparative HPLC is one of the significant chromatographic techniques where we can separate and purify omega-3 fatty acids, particularly ALA, EPA, and DHA 13,14 .…”

Section: Introductionmentioning

confidence: 99%

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Isolation of Alpha-linolenic acid (ALA) from tasar, Antherea mylitta pupal oil and its anticancer activity

Srivastava

Kumar

2023

J. Drug Delivery Ther.

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Previously, we reported that tasar silkworm pupal oil (TPO) is a rich source of alpha-linolenic acid (ALA) (37%). In this study, we have used three chromatographic techniques, namely preparative HPLC, gas chromatography-mass spectroscopy (GC-MS), and gas chromatography flame ionization detection (GC-FID), to purify, determine, and quantify alpha-linolenic acid (ALA), respectively, from TPO. Additionally, to determine the potential of the purified ALA as an anticancer drug, the sulforhodamine B (SRB) assay was utilised for in vitro investigation. Results revealed that ALA was successfully purified in a fraction of TPO by preparatory HPLC. It was discovered that only ALA was present in the purified fraction of ALA when it underwent GC-MS analysis, further confirming its purity. In addition, the quantification analysis using GC-FID showed that the concentration of ALA was 11760 ppm. Furthermore, anticancer analysis revealed purified ALA significantly inhibited the growth of a colon cancer cell line, (COLO-205) with a GI 50 ≤20. This is the initial investigation into isolating and purifying ALA from Tasar pupal oil. Keywords: Alpha-linolenic acid; GC-FID; HPLC; Omega-3Preparative, Tasar pupal oil.

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Section: Purification Of Alpha-linolenic Acid (Ala)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation of Alpha-linolenic acid (ALA) from tasar, Antherea mylitta pupal oil and its anticancer activity

Srivastava

Kumar

2023

J. Drug Delivery Ther.

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“…Fatty acid composition is a major factor that determines the quality of silkworm pupae oil, and the analysis of fatty acids content is thus important (Zou et al, ). Although non‐MS based methods such as HPLC (Vidhathri et al, ) and GC (Mentang, Jintasataporn, & Ohshima, ; Wang, Wu, Wang, Wang, & Wu, ) have been published for measuring α ‐linolenic acid and linoleic acid in pupa oil, non‐MS based methods are in general considered less sensitive and selective than MS‐based assays. Gong et al published a gas chromatography/mass spectrometry (GC–MS) method for the analysis of fatty acid composition in silkworm pupa (Gong, Sima, & Sun, ).…”

Section: Determination Of the Fatty Acid Compositionmentioning

confidence: 99%

Application of mass spectrometry in silkworm research—Review

Sun

et al. 2019

Biomedical Chromatography

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In this first mass spectrometry‐focused review paper, we will review current applications of mass spectrometry in the area of silkworm research. We will focus our review on the following two most important areas as they are currently being researched by scientists. Firstly, the proteomics of proteins in the process of silkworm lifecycle has generated knowledge about previous undetected proteins, some of which might possess therapeutic effects. Secondly, fatty acids, which are the other major components in silkworm, have several potential medical applications. We will also highlight potential areas warranting further investigation.

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Isolation and Detection of Alpha Linolenic Acid from Silkworm Pupal Residue Oil (Bombyx mori L.) Using HPLC

Cited by 2 publications

References 6 publications

Isolation of Alpha-linolenic acid (ALA) from tasar, Antherea mylitta pupal oil and its anticancer activity

Isolation of Alpha-linolenic acid (ALA) from tasar, Antherea mylitta pupal oil and its anticancer activity

Application of mass spectrometry in silkworm research—Review

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