Isolation and detection of<i>Listeria monocytogenes</i>in poultry meat by standard culture methods and PCR

Kureljušić, Jasna; Rokvić, N; Jezdimirović, Nemanja; Kureljušić, Branislav; Pisinov, Boris; Karabasil, Nedjeljko

doi:10.1088/1755-1315/85/1/012069

Cited by 6 publications

(4 citation statements)

References 15 publications

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“…Our findings indicate that the distribution of L. monocytogenes varied among different animal's species, with the highest occurrence in beef and poultry meat, respectively. These findings are inconsistent with previous studies regarding the presence of L. monocytogenes in animal species, whereby the highest occurrence was observed in poultry meat samples (Elmali, Can, & Yaman, ; Kureljušić et al, ). In , Islam, Husna, Islam, and Khatun, reported the distribution of L. monocytogenes among animal species at 8.33% in chicken, 16.66% in beef, and 8.33% in goats in in Bangladesh.…”

Section: Discussioncontrasting

confidence: 98%

Occurrence, serotypes, and characteristics of Listeria monocytogenes in meat and meat products in South Africa between 2014 and 2016

Matle

Mbatha

Lentsoane

et al. 2019

Journal of Food Safety

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Listeria monocytogenes is an intracellular pathogen that causes life-threatening disease called listeriosis. However, in South Africa (SA), there is a dearth of information concerning the potential role of meat and meat products as potential sources for human listeriosis. The aim of the study was to determine the occurrence and level of L. monocytogenes found in meat and meat products in SA and to characterize L. monocytogenes strains according to serogroups, antimicrobial resistance profiles and virulence genes. A total of 2,017 samples from imported and locally produced raw, processed and ready to eat meat were collected from 2014 to 2016 across nine provinces of SA. The samples for L. monocytogenes were isolated using microbiological techniques and real-time polymerase chain reaction (PCR). Antimicrobial resistance profiles of the isolates were determined by testing 19 antimicrobial impregnated discs using Kirby-Bauer disc diffusion method. Characterization in terms of serogroup typing and virulence profiling was done using conventional PCR. The overall occurrence of L. monocytogenes was 14.7% (296/2,017), which varied between meat collected on the domestic market (15.0%; 264/1,758) and directly at the three ports of entries (12.4%; 32/259). The contamination level of the positive samples ranged from 1 to 3.7 log CFU/g and 1 to 4.1 log CFU/g for samples collected from the domestic and imported meat, respectively. All positive isolates were serotyped by multiplex PCR, of which majority of the isolates belonged to molecular serogroup ½a-3a (45.5%), followed by 4b-4d-4e (24.2%), and ½c-3c (15.2%). Most of the isolates harbored the inlJ (98.7%) and ipa (95.6%) genes. However, at least one of the other internalin genes (inlB, inlC, and inlA) was present in most of the isolates. All the tested isolates showed resistance to at least 3 of the 19 antibiotics, with 5 (1.7%) of them displaying resistance to 13 of the 19 antibiotics. Resistance to streptomycin (99.0%), clindamycin (97.3%), fusidic acids (95.6%), nitrofurantoin (79.7%), and gentamycin (74.4%) was observed while high rates of sensitivity were observed for ampicillin (85.6%), kanamycin (84.6%), amikacin (77.6%), vancomycin (74.2%), and tetracycline (62.5%).The presence of L. monocytogenes in various meat products in SA pose a risk to human health. Therefore, the present research provides valuable baseline information that will help in the development of policies and regulations for monitoring of L. monocytogenes in meat and meat products in SA. Practical applicationsThis study is extensive and involved analysis of meat and meat products from all the nine provinces of South Africa. Furthermore, the study involved analysis of imported meat (from the three major ports of entry) that was destined for the South African market. The findings that were obtained are significant from academic, policy, and practical points of view. The findings provide empirical evidence of the contamination of a proportion of raw intact, raw processed, and ready-to-eat meat and produc...

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Section: Discussioncontrasting

confidence: 98%

Occurrence, serotypes, and characteristics of Listeria monocytogenes in meat and meat products in South Africa between 2014 and 2016

Matle

Mbatha

Lentsoane

et al. 2019

Journal of Food Safety

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“…After consumption, several contaminated food types, such as milk and milk products, vegetables, meat, and meat products, have been implicated in listeriosis sporadic cases and/or outbreaks [ 5 , 6 ]. Some clinical manifestations of listeriosis in humans include fever, muscle aches, nausea, vomiting, stomach cramps, diarrhea, abortion, preterm birth, stillbirth in pregnant women, meningitis or encephalitis, and death [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Genomic Characterization of Listeria innocua Isolates Recovered from Cattle Farms, Beef Abattoirs, and Retail Outlets in Gauteng Province, South Africa

Gana,

Gcebe,

Pierneef

et al. 2023

Pathogens

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Whole-genome sequencing (WGS) was used for the genomic characterization of one hundred and ten strains of Listeria innocua (L. innocua) isolated from twenty-three cattle farms, eight beef abattoirs, and forty-eight retail outlets in Gauteng province, South Africa. In silico multilocus sequence typing (MLST) was used to identify the isolates’ sequence types (STs). BLAST-based analyses were used to identify antimicrobial and virulence genes. The study also linked the detection of the genes to the origin (industries and types of samples) of the L. innocua isolates. The study detected 14 STs, 13 resistance genes, and 23 virulence genes. Of the 14 STs detected, ST637 (26.4%), ST448 (20%), 537 (13.6%), and 1085 (12.7%) were predominant, and the frequency varied significantly (p < 0.05). All 110 isolates of L. innocua were carriers of one or more antimicrobial resistance genes, with resistance genes lin (100%), fosX (100%), and tet(M) (30%) being the most frequently detected (p < 0.05). Of the 23 virulence genes recognized, 13 (clpC, clpE, clpP, hbp1, svpA, hbp2, iap/cwhA, lap, lpeA, lplA1, lspA, oatA, pdgA, and prsA2) were found in all 110 isolates of L. innocua. Overall, diversity and significant differences were detected in the frequencies of STs, resistance, and virulence genes according to the origins (source and sample type) of the L. innocua isolates. This, being the first genomic characterization of L. innocua recovered from the three levels/industries (farm, abattoir, and retail) of the beef production system in South Africa, provides data on the organism’s distribution and potential food safety implications.

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“…Listeriosis arises from food contamination and is associated with encephalitis, miscarriage, septicaemia and sometimes even results in death (Martinez et al, 2017). It commonly occurs more in pregnant women, immune-compromised individuals, HIV affected people and ageing adults (Kureljušić et al, 2017). This disease is one of the noted causes for deaths resulting from foodborne infections, with about 260 cases per year.…”

Section: Listeriamentioning

confidence: 99%

Molecular characterization of drug resistant Listeria monocytogenes from food and water samples

Das¹,

Mathuram²,

Meera³

et al. 2019

MJM

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Aims: Present research is focused on the molecular level characterization of drug-resistant Listeria monocytogenes identified from food and water samples from Tamil Nadu, India. Methodology and results: A total of 39 food and water samples were collected from local markets and retail shops in Tamil Nadu, India and processed for the isolation and identification of bacteria. Morphology of the bacteria was analysed under a fluorescent microscope. Isolated bacteria were serotyped and screened for the presence of virulence-associated genes haemolysin (hlyA) and invasive associated protein (iapA) by Real-time polymerase chain reaction. The qPCR positive isolates were also typed by random amplified polymorphic DNA-PCR for epidemiological study. Antibiotic resistance test was done with 16 commercial antibiotics by disc diffusion method. A total of 8 (20.51%) L. monocytogenes were identified belonging to the serotype group 1/2a, 1/2b, 1/2c and 4b. PCR assays revealed the presence of hlyA (456 bp) and iapA (131 bp) genes. In RAPD, OPA-10 primer was found to generate the distinct polymorphic fragment among the isolates. All the isolates were 100% resistant to rifampicin, co-methoxazole, linezolid and oxacillin and 100% sensitive to tetracycline and chloramphenicol. Tetracycline and chloramphenicol are suggested to be a very effective antibiotic against the tested L. monocytogenes isolates. Conclusion, significance and impact of study: The hlyA and iapA based quantitative PCR technique could be a rapid molecular technique for the detection of L. monocytogenes used in this study. Serotyping along with RAPD-PCR was able to discriminate between the isolates and therefore could serve as a robust and sensitive tool for typing antibioticresistant strains of L. monocytogenes.

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Isolation and detection ofListeria monocytogenesin poultry meat by standard culture methods and PCR

Cited by 6 publications

References 15 publications

Occurrence, serotypes, and characteristics of Listeria monocytogenes in meat and meat products in South Africa between 2014 and 2016

Occurrence, serotypes, and characteristics of Listeria monocytogenes in meat and meat products in South Africa between 2014 and 2016

Genomic Characterization of Listeria innocua Isolates Recovered from Cattle Farms, Beef Abattoirs, and Retail Outlets in Gauteng Province, South Africa

Molecular characterization of drug resistant Listeria monocytogenes from food and water samples

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