Isolation and detection of Leishmania species among naturally infected Rhombomis opimus, a reservoir host of zoonotic cutaneous leishmaniasis in Turkemen Sahara, North East of Iran

Mirzaei, Asad; Rouhani, Soheila; Taherkhani, H; Farahmand, Mahin; Kazemi, Bahram; Mahdi, Hedayati; Baghaei, Ahmad; Davari, Behroz; Parvizi, Parviz

doi:10.1016/j.exppara.2011.08.020

Cited by 41 publications

(40 citation statements)

References 23 publications

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“…However, the above method definitely lacks the efficiency of new methods as determining the nucleotide sequence of the gene under study and phylogenetic analysis (Davami et al 2010;Muller and Schlein 2011;Strelkova et al 2001;Mirzaei et al 2011;Hajjaran et al 2009). …”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of Leishmania parasites isolated from sandflies species of a zoonotic cutaneous leishmaniasis in Musiyan south west Iran

et al. 2016

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Section: Introductionmentioning

confidence: 99%

Molecular characterization of Leishmania parasites isolated from sandflies species of a zoonotic cutaneous leishmaniasis in Musiyan south west Iran

et al. 2016

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“…In Iran, R. opimus is the main reservoir of ZCL in the centre and north-east of the country. Several Leishmania infections due to L. major have been reported from R. opimus in endemic and non-endemic foci of ZCL throughout the country (Yaghoobi-Ershadi et al 1996, Mohebali et al 2004, Mirzaei et al 2011.…”

Section: Identification Of the Natural Hosts Of Leishmaniamentioning

confidence: 99%

“…Four important foci of the disease based on rodent reservoir hosts have been reported in Iran: (i) central and north-east of Iran, where Rhombomys opimus Lichtenstein (Rodentia: Gerbillinae) is the main reservoir of the disease (Javadian et al 1976, Gramiccia and Gradoni 2005, Mirzaei et al 2011; (ii) west and south-west of Iran, where Tatera indica Hardwicke (Rodentia: Gerbillinae) (Indian jird) replaced R. opimus and plays an important role as reservoir (Javadian et al 1988); (iii) south-eastern Iran, where Meriones hurrianae Jerdon (Rodentia: Gerbillinae) was reported as a natural reservoir host (Kassiri et al 2011), and (iv) southern Iran, where Meriones libycus erythrourus Lichtenstein (Rodentia: Gerbillinae) is considered the primary and main reservoir host of the disease, whereas R. opimus and T. indica are absent (Rassi et al 2006(Rassi et al , 2007.…”

Section: Identification Of the Natural Hosts Of Leishmaniamentioning

confidence: 99%

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Molecular characterization of Leishmania spp. in reservoir hosts in endemic foci of zoonotic cutaneous leishmaniasis in Iran

Akhoundi¹,

Mohebali²,

Asadi³

et al. 2013

FOLIA PARASIT

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Zoonotic cutaneous leishmaniasis (ZCL) is an expanding disease and a public health issue in Iran. In the present study, rate of natural infection of rodent populations with Leishmania was investigated in six endemic foci including 28 villages in Golestan, Esfahan, Yazd, Fars, Khuzestan and Ilam provinces. A total of 593 rodents were captured and identified as Rhombomys opimus (n = 325), Meriones libycus (n = 171), Meriones persicus (n = 27), Tatera indica (n = 37), Nesokia indica (n = 12), Rattus rattus (n = 13) and Mus musculus (n = 8). Microscopic examinations of Giemsa-stained smears showed that 108 out of 593 (18.2%) rodents were infected with Leishmania spp., whereas infection of 186 out of 593 (31.4%) rodents with Leishmania was then confirmed by ITS1-PCR. The highest rate of infection was found in R. opimus (prevalence of 35%) and M. libycus (31%).

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“…(Karamian et al 2008) Golestan Province, Northern Iran, is a hyperendemic region of CL. In this area, common causative agents of CL had been recognized as L. major (Rassi et al 2008;Mirzaei et al 2011;Pagheh et al 2012).…”

Section: Introductionmentioning

confidence: 99%

An improved microculture method for diagnosis of cutaneous leishmaniasis

et al. 2013

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This study evaluated the performance of three diagnostic methods for cutaneous leishmaniasis (CL). Patients who came to the Health Center Laboratory of Gonbad-e-Qabus in Golestan Province, Iran, were enrolled in the study. Skin scraping smear, improved microculture (IMC) and polymerase chain reaction (PCR) were performed. A total of 303 subjects were recruited, among whom 273 subjects fulfilled the criteria for CL. Sensitivity and specificity were 88.8 % (95 % CI = 84.2-92.2 %) and 100.0 % for smears, 98.4 % (95 % CI = 96.1-99.1 %) and 100.0 % for IMC, both of them 100.0 % for PCR. Although, PCR was relatively more sensitive than the IMC, the high correlation (agreement = 96 %, Kappa = 0.82) between IMC and PCR along with the advantages of simplicity, rapidity, adequate sensitivity and as a needle free method, offers the IMC as a valuable alternative diagnostic method for PCR in diagnosis of CL.

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Isolation and detection of Leishmania species among naturally infected Rhombomis opimus, a reservoir host of zoonotic cutaneous leishmaniasis in Turkemen Sahara, North East of Iran

Cited by 41 publications

References 23 publications

Molecular characterization of Leishmania parasites isolated from sandflies species of a zoonotic cutaneous leishmaniasis in Musiyan south west Iran

Molecular characterization of Leishmania parasites isolated from sandflies species of a zoonotic cutaneous leishmaniasis in Musiyan south west Iran

Molecular characterization of Leishmania spp. in reservoir hosts in endemic foci of zoonotic cutaneous leishmaniasis in Iran

An improved microculture method for diagnosis of cutaneous leishmaniasis

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