Isolation and determination of bacterial microbiota of Varroa destructor and isolation of Lysinibacillus sp. from it

Abstract

Background The importance of bees for environmental health is known. Within the scope of this importance, it is of great importance to protect the health of bees and to prevent colony extinction. In this context, it is very important to develop effective methods in combating microorganisms, parasitoids, mites and organisms that cause disease or harm in bees. Both use different methods in terms of bee health. Result In this study, the possibility of the bacteria isolated from Varroa destructor mite being bioinsecticide was investigated. Accordingly, six bacteria were isolated from the mite. Isolated bacteria were analyzed according to biochemical tests, molecular analysis, optimum growth pH and phylogenetic tree drawn as Pantoea dispersa (GV1), Lysinibacillus macroides (GV3), Bacillus mycoides (GV4), Lysinibacillus fusiformis (GV5), Pseodomonas lutea (GV5), Lysinibacillus varians (GV7). Lysinibacillus sp. The entomopathogenic feature of Lysinibacillus sp. ranked it as the most important species. When the insecticidal properties of bacteria were examined, they were determined as 53, 90, 62, 95, 74 and 83% for GV1, GV3, GV4, GV5, GV6 and GV7, respectively. Conclusion Based on these results and literature review, Lysinibacillus sp. species had a high potential to be used as bioinsecticide against V. destructor mite.

Entomopathogenic potential of bacteria associated with soil-borne nematodes and insect immune responses to their infection

“…fusiformis strains have a low entomopathogenic effect against Spodoptera exigua Hübner (Lepidoptera: Noctuidae) [ 84 ]. However, other strains of this species are highly lethal to Drosophila melanogaster and show miticidal effect against Varroa destructor [ 85 , 86 ]. These previous literature reports together with our results indicate a great entomopathogenic potential of L .…”

Determination of Antimicrobial Effects of Secondary Metabolites of Different Bacteria Belonging to the Genus Bacillus

“…The bacterial strains in this study have been previously isolated and determined (Usta 2021b(Usta , 2021a. Supernatant samples containing secondary metabolites were obtained from these previously described bacteria (Table 1).…”

“…These pathogens cause the death of insects by synthesizing compounds that will cause various diseases. There are many studies on insect pathogens (Miller et al 2021, Steele et al 2021, Usta 2021b). However, more information is needed on whether the secondary metabolites produced by these pathogens have lethal or growth-inhibitory effects.…”