Isolation and determination of four potential antimicrobial components from <i>Pseudomonas aeruginosa</i> extracts

Xu, Ling; Zeng, Jian Wen; Jiang, Chong He; Wang, Huan; Li, Yu Zhen; Wen, Wei; Li, Jie Hua; Wu, Feng; Wei, Ting; Sun, Zi Yong; Huang, Chih‐Yang

doi:10.7150/ijms.18896

Cited by 14 publications

(12 citation statements)

References 25 publications

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“…Bandara, Yau [34] reported that the interaction among P. aeruginosa and 5 non-C. albicans Candida species was variable and species-specific; a finding that is in agreement with our results. Also, in keeping with our observations, variation in the effect of the tested P. aeruginosa on the tested Candida species was reported by Xu, Zeng [10]. In particular, some P. aeruginosa exhibited high antifungal activity and some showed partial or no activity against the tested Candida spp.…”

Section: Plos Onesupporting

confidence: 91%

“…Also, in keeping with our observations, variation in the effect of the tested P . aeruginosa on the tested Candida species was reported by Xu, Zeng [ 10 ]. In particular, some P .…”

Section: Resultssupporting

confidence: 59%

“…aeruginosa virulence factors in the development and the severity of disease and in relation to the killing effect on Candida Spp. [ 10 , 38 ]. Caldwell, Chen [ 35 ] and Taylor, Machan [ 39 ] showed that type IV pili, phospholipase C, phenazines, pyocyanin and pseudomonas quinolones secreted molecules showed inhibiting activity on Candida species, a finding which is in agreement with our results.…”

Section: Resultsmentioning

confidence: 99%

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Virulence profiles of some Pseudomonas aeruginosa clinical isolates and their association with the suppression of Candida growth in polymicrobial infections

et al. 2020

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Pseudomonas aeruginosa is an opportunistic pathogen that can cause a variety of diseases especially in the hospital environment. However, this pathogen also exhibits antimicrobial activity against Gram-positive bacteria and fungi. This study aimed to characterize different virulence factors, secreted metabolites and to study their role in the suppression of Candida growth. Fifteen P. aeruginosa isolates were tested for their anticandidal activity against 3 different Candida spp. by the cross-streak method. The effect on hyphae production was tested microscopically using light and scanning electron microscopy (SEM). Polymerase chain reaction was used in the detection of some virulence genes. Lipopolysaccharide profile was performed using SDS-polyacrylamide gel stained with silver. Fatty acids were analyzed by GC-MS as methyl ester derivatives. It was found that 5 P. aeruginosa isolates inhibited all tested Candida spp. (50–100% inhibition), one isolate inhibited C. glabrata only and 3 isolates showed no activity against the tested Candida spp. The P. aeruginosa isolates inhibiting all Candida spp. were positive for all virulence genes. GC-Ms analysis revealed that isolates with high anticandidal activity showed spectra for several compounds, each known for their antifungal activity in comparison to those with low or no anticandidal activity. Hence, clinical isolates of P. aeruginosa showed Candida species-specific interactions by different means, giving rise to the importance of studying microbial interaction in polymicrobial infections and their contribution to causing disease.

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Section: Plos Onesupporting

confidence: 91%

“…Also, in keeping with our observations, variation in the effect of the tested P . aeruginosa on the tested Candida species was reported by Xu, Zeng [ 10 ]. In particular, some P .…”

Section: Resultssupporting

confidence: 59%

Section: Resultsmentioning

confidence: 99%

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Virulence profiles of some Pseudomonas aeruginosa clinical isolates and their association with the suppression of Candida growth in polymicrobial infections

et al. 2020

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“…These findings are in concordance with studies that have shown P. aeruginosa to produce antimicrobial exoproducts effective against S. aureus. 24,[32][33][34] The layered woundlike growth medium contained laked horse blood, cattle serum, and peptone, which is similar to wound-simulating media used in several studies. 20,24,25 According to Thaarup and Bjarnsholt, a suitable medium simulating wound fluid should consist of a meat-based broth combined with mammal serum and red blood cells, because the wound bed is recognized to contain mainly damaged host tissue, plasma and red blood cells.…”

Section: Biofilm Model Developmentmentioning

confidence: 99%

A novel chronic wound biofilm model sustaining coexistence of Pseudomonas aeruginosa and Staphylococcus aureus suitable for testing of antibiofilm effect of antimicrobial solutions and wound dressings

Chen

Lorenzen

et al. 2021

Wound Repair Regeneration

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Chronic wounds are a large burden to patients and healthcare systems. Biofilm infections in chronic wounds are crucial factors leading to non-healing of wounds. It is important to study biofilm in wounds and to develop effective interventions against wound biofilm. This study presents a novel in vitro biofilm model mimicking infected chronic wounds. The novel layered chronic wound biofilm model uses woundlike media and includes both Pseudomonas aeruginosa and Staphylococcus aureus, which have been identified as the most important pathogens in wounds. The model sustains their coexistence for at least 96 h. Microscopy of the model revealed microbial growth in non-surface attached microcolonies as previously observed in vivo. The model was used to determine log 10 -reduction for the use of an antimicrobial solution and antimicrobial dressings (containing silver or honey) showing moderate-to-low antibiofilm effect, which indicates better concordance with the observed clinical performance of this type of treatment than other widely used standard tests. K E Y W O R D S biofilm, coexistence of Pseudomonas aeruginosa and Staphylococcus aureus, model, test of antibiofilm effect | INTRODUCTIONChronic wounds constitute a large burden on patients, society and healthcare systems, affecting 2% of the population in the developed countries with an estimated 2%-3% of the healthcare budgets related to chronic wounds globally, equalling US $13-15 billion annually. [1][2][3] Bacteria inflict on the healing of chronic wounds, 4 with Pseudomonas aeruginosa being identified as a major pathogen 5 together with Staphylococcus aureus, but the underlying mechanisms on the pathogenicity of wound biofilm are not well understood. 6 Furthermore, the Chen and Lorenzen served as co-first authors.

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“…The discovery of penicillin in 1928 revealed that microorganisms may contribute to known existing issues, due to the abundant bioactive substances produced within their cells (L.-Q. Xu et al, 2017). Some pathogens have been shown to develop antimicrobial-resistant properties, leading to increased morbidity, mortality, and healthcare costs (Pratiwi et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Untitled

2021

JTAS

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The agricultural industry worldwide faces challenges in the struggle against plant diseases. In efforts to increase agricultural intensities, the dependency on agrochemicals for crop protection has become significantly high. Moreover, the increasing use of agrochemicalbased products has resulted in multidrug-resistant pathogens and environmental pollution. This paper reviews the biocontrol capacity of plant growth-promoting microorganisms (PGPMs) originating from plants towards plant pathogens. The current trend in discovering new compounds has shown antimicrobial activity gaining immense interest due to its vast potential. On a related note, PGPMs are an aspect of that research interest that can be further explored as antimicrobial producers. In this work, the types of biocontrol mechanisms pertaining to PGPMs as well as their roles in biocontrol activity were covered. A biocontrol approach exploits disease-suppressive microorganisms to improve plant health by controlling related pathogens. The understanding of these microorganisms and mechanisms of pathogen antagonismare primary factors in ensuring improvement for future applications. Inevitably, there is indeed room for rigorous expansion with respect to PGPMs in the future of agriculture.

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Isolation and determination of four potential antimicrobial components from Pseudomonas aeruginosa extracts

Cited by 14 publications

References 25 publications

Virulence profiles of some Pseudomonas aeruginosa clinical isolates and their association with the suppression of Candida growth in polymicrobial infections

Virulence profiles of some Pseudomonas aeruginosa clinical isolates and their association with the suppression of Candida growth in polymicrobial infections

A novel chronic wound biofilm model sustaining coexistence of Pseudomonas aeruginosa and Staphylococcus aureus suitable for testing of antibiofilm effect of antimicrobial solutions and wound dressings

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