Isolation and diagnosis of Streptococcus mutans and Streptococcus sorbinus from patients with tooth decay and gingivitis

Abstract

The Streptococcus bacteria Streptococcus sobrinus and Streptococcus mutans are one of the most common types in the mouth, where tooth decay, necrosis and gingivitis are among the health problems that the Iraqi society suffers from, as the treatment of bacteria has become a challenge due to their ability to resist many antibiotics. The study aimed to isolate and diagnose Streptococcus mutans and Streptococcus sobrinus and study their resistance to a number of antibiotics, and evaluate the susceptibility of Streptococcus mutans and Streptococcus sobrinus isolates in biofilms. Using biochemical tests and phenotypic diagnostics. The study included the collection of (275) oral swabs from patients with tooth decay from the area of caries on the surface of the tooth and from cysts for infected patients and visitors at the Medical City Hospital in Baghdad Governorate with ages ranging between (6-70) years and of both sexes for the period from 1/9/2022 to 1/11/2022. the number of isolates was 6 (2.18%) belonging to Streptococcus mutans and only one isolate (0.36%) of Streptococcus sobrinus, the sensitivity of all the isolates of the bacteria under study was tested towards 10 antibiotics, and the results showed that the isolates were resistant to most of the antibiotics used in this study, and all the isolates were chosen to test their ability to form biofilms using the microtitration plate, and the results showed that most of the isolates were strong in the formation of biofilms.