Isolation and evaluation of the radical-scavenging activity of the antioxidants in the leaves of an edible plant, Mallotus japonicus

Tabata, Hiromasa; Katšube, Takao; Tsuma, Terumi; Ohta, Yutaka; Imawaka, Naoto; Utsumi, Toshihiko

doi:10.1016/j.foodchem.2007.12.017

Cited by 46 publications

(31 citation statements)

References 23 publications

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“…At present, natural products exhibiting a wide range of biological activities, such as antioxidant, antiinflammatory, and antitumor effects, are promising candidates for treating UV-damaged skin. Previously, we had conducted a systematic screening focusing on antioxidant activities and had found that among 52 edible plant extracts, Mallotus japonicus leaf extract exhibits the strongest activity [6,7]. Our screening had also identified that the antioxidant activities of 60% aqueous ethanol extract of Aesculus hippocastanum (horse chestnut) seed shell extract are comparable to those of M. japonicus leaf extract.…”

Section: Introductionmentioning

confidence: 84%

Preliminary study on antioxidant properties, phenolic contents, and effects of Aesculus hippocastanum (horse chestnut) seed shell extract on in vitro cyclobutane pyrimidine dimer repair

Makino¹,

Katšube²,

Ohta³

et al. 2017

J Intercult Ethnopharmacol

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Aim: Human skin exposure to solar ultraviolet (UV) radiation induces cells to produce reactive oxygen species that directly cause DNA damage via the formation of cyclobutane pyrimidine dimers (CPDs), which are the primary UVB-induced DNA lesions. CPDs are responsible for cell death, mutation, and neoplastic transformation. Aesculus hippocastanum (horse chestnut) seed extract has traditionally been used for venotonic treatments and also as a raw material for cosmetics. This study aimed to characterize the antioxidant properties of the phenolic contents of extracts from A. hippocastanum seed shell and endosperm and to investigate their effects on CPD repair in UVB-exposed human dermal fibroblasts in vitro. Materials and Methods: Crude 60% aqueous ethanol extracts (v/v) were prepared, and their total polyphenol contents, antioxidant activities, and oxygen radical absorbance capacity (ORAC) values were measured by Folin-Ciocalteu, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging capacity, and ORAC assays, respectively. The levels of CPDs induced by UVB irradiation were measured by cell-based immunoassays after 6 h treatment with the extract of A. hippocastanum seed shell at a concentration of 100 µg/ml and 9 h treatment with it at concentrations of 50, 100, and 200 µg/ml. Results: A. hippocastanum seed shell extract had 602 ± 1.6 mg gallic acid equivalent per gram of extract and exhibited 4990 ± 70.9 and 7140 ± 835 µmol Trolox equivalents per gram of extract as DPPH radicalscavenging activity and ORAC value, respectively; these values were comparable to those of extracts from green tea and Mallotus japonicus leaves. However, these values of the seed endosperm extract were relatively low. A 9 h treatment of cells with seed shell extracts at concentrations of 50, 100, and 200 µg/ml after UVB exposure significantly reduced CPD levels compared with those in UVB-exposed cells without treatment. Conclusion: This study indicated that A. hippocastanum seed shell extract possesses a potential to limit the harmful effects of human skin exposure to UVB and that this could be the first step toward identifying novel benefits of this extract.

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Section: Introductionmentioning

confidence: 84%

Preliminary study on antioxidant properties, phenolic contents, and effects of Aesculus hippocastanum (horse chestnut) seed shell extract on in vitro cyclobutane pyrimidine dimer repair

Makino¹,

Katšube²,

Ohta³

et al. 2017

J Intercult Ethnopharmacol

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“…Cumulative release of gallic acid from gallic acid-loaded electrospun PLLA fiber mats, in terms of the percentage of the weight of gallic acid released divided by the actual weight of gallic acid in the specimens, as a function of submersion time in three different types of releasing medium, i.e., acetate buffer, citrate-phosphate buffer, and normal saline, at skin temperature of 32 8C (n ¼ 5). [29][30][31] Gallic acid, which acts as a donor of a hydrogen atom or an electron, can transform a DPPH radical into its reduced form DPPH ÀH, [32] as illustrated in Scheme 1. The antioxidant activity of the as-loaded gallic acid in the gallic acid-loaded PLLA fiber mats was 85.4 AE 1.3%.…”

Section: Antioxidant Activity Of Gallic Acidmentioning

confidence: 99%

Gallic Acid‐Loaded Electrospun Poly(L‐Lactic Acid) Fiber Mats and their Release Characteristic

Chuysinuan

Chimnoi

Techasakul

et al. 2009

Macro Chemistry & Physics

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Ultra‐fine fiber mats of poly(L‐lactic acid) containing gallic acid were prepared by electrospinning from gallic acid‐containing PLLA solution in 7:3 v/v dichloromethane (DCM)/N,N‐dimethylformamide (DMF). The amount of the as‐loaded gallic acid was 40% w/w (based on the weight of PLLA in the solution) or 28.6 wt.‐% (based on the weight of the resulting fiber mats). Both the neat and the gallic acid‐loaded PLLA fibers were smooth, with the average diameters of 965 and 843 nm, respectively. No aggregates of gallic acid were observed on the fiber surface and the actual amount of gallic acid in the gallic acid‐loaded PLLA fiber mats, determined in the acetate buffer, the citrate‐phosphate buffer, and the normal saline, was 26.3, 27.1, and 24.6 wt.‐%. The cumulative amount of gallic acid released from the gallic acid‐loaded PLLA fiber mats was greatest in the normal saline, followed by those in the citrate‐phosphate and the acetate buffer, respectively. Lastly, the free radical scavenging activity, based on the 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) assay, of the as‐loaded and the as‐released gallic acid remained active.magnified image

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“…Raharivelomanana, Bianchini, Boura- ima-Madjebi, & Hnawia, 2005;Gao, Huang, Xu, & Kawabata, 2007;Tabata et al, 2008) while 3 and 5 are novel ellagitanins.The molecular formula of 3 was deduced from the negative ESI-HR-MS data of its deprotonated anion,[MÀH] À : m/z 1121.0968 (calcd. for C 48 H 33 O 32 , 1121.0955) as C 48 H 34 O 32 .…”

mentioning

confidence: 99%

Novel α-glucosidase inhibitors from Macaranga tanarius leaves

Gunawan-Puteri

Kawabata

2010

Food Chemistry

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Isolation and evaluation of the radical-scavenging activity of the antioxidants in the leaves of an edible plant, Mallotus japonicus

Cited by 46 publications

References 23 publications

Preliminary study on antioxidant properties, phenolic contents, and effects of Aesculus hippocastanum (horse chestnut) seed shell extract on in vitro cyclobutane pyrimidine dimer repair

Preliminary study on antioxidant properties, phenolic contents, and effects of Aesculus hippocastanum (horse chestnut) seed shell extract on in vitro cyclobutane pyrimidine dimer repair

Gallic Acid‐Loaded Electrospun Poly(L‐Lactic Acid) Fiber Mats and their Release Characteristic

Novel α-glucosidase inhibitors from Macaranga tanarius leaves

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