Isolation and Examination of the Host Range of the Pseudomonas aeruginosa Bacteriophages Isolated from Sewage

Zaman, Najat A.

doi:10.33899/rjs.2010.6940

Cited by 2 publications

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“…Many in vitro studies have been conducted in recent years to evaluate the prospective of phages against clinical isolates of P. aeruginosa, including MDR strains, in planktonic cultures or biofilms [3].Local and international studies interested with isolation of aerophage.Zaman(2010) isolated P. aeruginosa phage and isolates with green and yellow coloring on Nutrient agar were lytic by isolated phages in Kirkuk [8]. Alkhozai and Alkabei (2011) isolated Five types of phages specific to P. aeruginosa from (60) sample of sewage water primarily named (ZM1,ZM2,ZM3,ZM4,ZM5) in Al-Diwanyia City [9].…”

Section: Discussionmentioning

confidence: 99%

Bacteriolytic Activity study of Aerophage on Pseudomonas aeruginosa

Jameel¹

2018

DJM

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Background:The increasing incidence of antibiotic resistance in bacterial pathogens has justified a reassessment of the value of phages as antibacterial agents for medical applications. Objective: To assess the effect of bacteriolytic activity against P. aeruginosa by use multi methods for isolation of bacteriophage. Patients and Methods: This study was performed from from November, 2016 till April, 2017. A total of 60 samples collected from urine, stool, diabetes leg, ear, burns and wounds infections in General Baqubah Hospital to isolate Pseudomonas aeruginosa and tested for 6 groups of antibiotics. Aerophage was isolated using three methods: spotting; double -layer agar plate and liquid broth method. After enrichment of aerophage, the bacteriolytic activity of aerophage was done by spotting method. Results: Pseudomonas aeruginosa isolates resistance were 10(43.5%) for Aztreonem and 8(34.8 %) to Ticarcillin-Claculanic acid followed by Imipenem 4(17.4%), Gentamicin and when the study done Levofloxacin 3(13%) for each finally Cefepime2(8.7%). high significant differences of aztreonem with both of cefepim and gentamycin (P<0.01) while significant differences of aztreonem with both imipenim and levofloxacillin (P<0.05%). while the results of bacteriolytic activity showed all ear and leg diabetes isolates were sensitive to aerophage (100%) followed by urine isolates(75%), stool isolates(50%) and burns isolates(25%) while all wound isolates were resist to aerophage(100%). The result of relation between Antibiotic resistance phenotype profile and Aerophage sensitivity that no detect to susceptibility of isolates to aerophage but the source of isolates may be detected to infection with aerophage. Conclusion:Aztreonem antibiotic was widely used in Baqubah city recently. All three methods to isolate aerophage were effective. The result of relation between Antibiotic resistance phenotype profile and Aerophage sensitivity that no detect to susceptibility of isolates to aerophage but the source of isolates may be detected to infection with aerophage.

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Section: Discussionmentioning

confidence: 99%

Bacteriolytic Activity study of Aerophage on Pseudomonas aeruginosa

Jameel¹

2018

DJM

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Inhibitory Effect of Bacteriophages Isolated from Sewage Water in the City of Kirkuk on some Types of Human Pathogenic Bacteria

Al-Tae¹,

Al-Hammadi²

2017

Baghdad Sci.J

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Most approaches to combat antibiotic resistant bacteria concentrate on discovering new antibiotics or modifying existing ones. However, one of the most promising alternatives is the use of bacteriophages. This study was focused on the isolation of bacteriophages that are specific to some of commonly human pathogens namely E. coli, Streptococcus pyogenes, Staphylococcus aureus, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella spp. and Klebsiella pneumoniae. These bacteriophages were isolated from sewages that were collected from four different locations in Kirkuk City. Apart from S. pyogenes, bacteriophages specific to all tested bacteria were successfully isolated and tested for their effectiveness by spot test. The most effective bacteriophages that were isolated from sewages and sewage water of Al-Jumhori Hospital compared to other sites. It is concluded that the sewage water of hospitals represents a perfect environment for these bacteriophages.

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Isolation and Examination of the Host Range of the Pseudomonas aeruginosa Bacteriophages Isolated from Sewage

Cited by 2 publications

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Bacteriolytic Activity study of Aerophage on Pseudomonas aeruginosa

Bacteriolytic Activity study of Aerophage on Pseudomonas aeruginosa

Inhibitory Effect of Bacteriophages Isolated from Sewage Water in the City of Kirkuk on some Types of Human Pathogenic Bacteria

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