2014
DOI: 10.3791/52172
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Isolation and Excision of Murine Aorta; A Versatile Technique in the Study of Cardiovascular Disease

Abstract: Cardiovascular disease is a broad term describing disease of the heart and/or blood vessels. The main blood vessel supplying the body with oxygenated blood is the aorta. The aorta may become affected in diseases such as atherosclerosis and aneurysm. Researchers investigating these diseases would benefit from direct observation of the aorta to characterize disease progression as well as to evaluate efficacy of potential therapeutics. The goal of this protocol is to describe proper isolation and excision of the … Show more

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Cited by 8 publications
(4 citation statements)
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“…Mouse aortas were isolated as described previously 19 . Extraction of primary mouse cells was accomplished via tissue digestion and magnetic bead isolation.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Mouse aortas were isolated as described previously 19 . Extraction of primary mouse cells was accomplished via tissue digestion and magnetic bead isolation.…”
Section: Methodsmentioning
confidence: 99%
“…Mouse aortas were isolated as described previously. 19 Extraction of primary mouse cells was accomplished via tissue digestion and magnetic bead isolation. Briefly, isolated aortas were then minced into smaller sections and then placed into 1 ml of 1 mg/ml collagenase IV and dispase for 45 min and then resuspended in 10 ml of 0.4% BSA-DPBS to halt digestion.…”
Section: Isolation Of Primary Mouse Primary Cellsmentioning
confidence: 99%
“…Fifteen healthy male NMRI mice were sacrificed by Ketamine/Xylazine-induced anesthesia (50 mg/kg ketamine, 5 mg/kg xylazine). The thoracic aorta was isolated and excised from the animal body according to a comprehensive protocol provided by Robbins et al [20]. The fresh isolated thoracic aorta was flushed twice with the phosphate-buffered solution (PBS, Merck, Germany) containing amphotericin B and antibiotics.…”
Section: Preparation Of Acellular Aortic Matrices (Aams)mentioning
confidence: 99%
“…We obtained the mouse aortas as previous reported [27]. This method has no effect on the aortic adventitial integrity and thickness measurements.…”
Section: Histological Analysismentioning
confidence: 99%