Isolation and expression analysis of a homolog of the 14–3–3 epsilon gene in the diamondback moth, Plutella xylostella

Abstract

A full-length 14-3-3 gene homolog (also referred to as the Px14-3-3 epsilon "ε" or Px14-3-3ε gene) was cloned from cDNA of the diamondback moth, Plutella xylostella. The Px14-3-3 transcript is 789 nucleotides in length, and the predicted polypeptide is 263 amino acids in length, with a calculated molecular mass of 29.6 kDa. The Px14-3-3 gene contains the typical and predicted 14-3-3 domains and motifs. The amino acid sequence of the diamondback moth 14-3-3 gene is very similar to that of other insect epsilons (ε) but not to other insect zetas (ζ). In particular, the protein sequence of the Px14-3-3 gene shows high identity to the Bombyx mori epsilon (96.2%). Western blot analysis using an antibody against Px14-3-3ε verified the expression of 14-3-3ε in the larval, pupal, and adult stages. The Px14-3-3ε expression patterns in all the different tissue types were examined in the fourth instar larvae. Px14-3-3ε was detected in every tissue examined, including the body fat, hemocytes, brain, gut, and cuticle.

The Silencing of a 14-3-3ɛ Homolog in Tenebrio molitor Leads to Increased Antimicrobial Activity in Hemocyte and Reduces Larval Survivability

“…This finding is consistent to the previous report of Dm14-3-3 ɛ expressed during development and also in almost all embryonic and larval tissues [7]. The P. xylostella 14-3-3ɛ (Px14-3-3ɛ) protein also shows a constant expression in various tissue types and during the developmental stages of the moth [8]. The broad expression patterns of 14-3-3ɛ proteins can be reflected in their regulatory function in modulating many cellular processes [12,41].…”

“…The Drosophila 14-3-3ɛ (Dm14-3-3ɛ) and 14-3-3ζ (Dm14-3-3 ζ ) isoforms show a high sequence identity of 82% and 88% to their respective mammalian orthologues [6,7]. Homologs of the 14-3-3ɛ and 14-3-3ζ gene are also characterized from lepidopteron models such as Plutella xylostella [8] and Spodoptera litura [9], respectively. The silkworm 14-3-3 proteins (Bm14-3-3ɛ and Bm14-3-3ζ) show a sequence identity of almost 85%–90% with Drosophila 14-3-3 isoforms [10].…”

IDENTIFICATION AND EXPRESSION ANALYSIS OF TWO 14‐3‐3 PROTEINS IN THE MOSQUITO Aedes aegypti, AN IMPORTANT ARBOVIRUSES VECTOR

“…Hydra vulgaris, B. mori, Penaeus monodon, and Plutella xylostella only two 14-3-3 isoforms are also present (Wang and Shakes, 1997;Kong et al, 2007;Pauly et al, 2007;Tabunoki et al, 2008;Yoo et al, 2011;Kaeodee et al, 2011).…”