Isolation and Expression Analysis of a SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) Gene in Curcuma alismatifolia Gagnep.

Sucharitakul, Kannaporn; Rakmit, Rungroj; Boonsorn, Yada; Leelapon, Oranuch; Teerakathiti, Tharathorn; Bunnag, Sumontip; Chanvivattana, Yindee

doi:10.5539/jas.v6n10p207

Cited by 4 publications

(3 citation statements)

References 27 publications

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“…This result corroborates previous works conducted on Zea mays (Baudino et al, 2001), Triticum aestivum (Singla et al, 2008) Medicago truncatula (Nolan et al, 2003), and Trifolium nigrescens (Pilarska et al, 2016). Meanwhile, the expression of SERK is closely related to the induction of embryogenesis (Pérez-Núñez et al, 2009;Salvo et al, 2014;Pilarska et al, 2016) and the further development of embryos (Nolan et al, 2003;De Oliveira Santos et al, 2005;Sucharitakul et al, 2014). This gene may play a broader developmental role, such as in vitro organogenesis, plant growth, and development (Nolan, 2003;Savona et al, 2012).…”

Section: Discussionsupporting

confidence: 90%

“…Cdc2 gene expression precedes cell division, suggesting that it reflects a physiological state of competent cells to divide (Hemerly et al, 1993). Somatic embryogenesis, a form of in vitro organogenesis, is controlled by the SERK (somatic embryogenesis receptor kinase) genes detected in numerous species (Savona et al, 2012;Ma et al, 2014;Sucharitakul et al, 2014;Pilarska et al, 2016). The SERK gene encodes a transmembrane protein kinase belonging the family of leucine-rich repeat protein receptor-like kinases and it is upregulated by auxin (Zhang et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Molecular investigations of the somatic embryogenesis recalcitrancein the cherry (Prunus cerasus L.) rootstock CAB 6P

Mahmoud¹,

Jedidi²,

Delporte³

et al. 2017

Turk J Biol

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IntroductionSomatic embryogenesis is the process by which somatic cells, under induction conditions, generate embryogenic cells that go through a series of morphological and biochemical changes that result in the formation of somatic embryos (Quiroz-Figueroa et al., 2006). This process is feasible because plants possess cellular totipotency, by which individual somatic cells can regenerate a whole plant (Vasil and Vasil, 1972). The acquisition of embryogenic competence by somatic cells is a complex process that comprises several phases: dedifferentiation, cell reactivation, cell divisions, and various metabolic and developmental reprogramming steps (Ochatt, 2010). The technique of embryogenesis makes an attractive tool not only for mass production but also for crop improvement and genetic transformation (Pérez-Clemente et al., 2004;Quiroz-Figueroa et al., 2006;Namasivayam, 2007). However, the initiation of somatic embryogenesis in plants depends on both extrinsic and intrinsic factors.Among the various extrinsic factors that may induce an embryogenic pathway of development, plant growth regulators, especially auxins, have been known to be implicated in cell cycle regulation, cell division, and differentiation (Del Pozo and Manzano, 2014) as well as in the induction of somatic embryogenesis (Pasternak, 2002). Picloram (4-amino-3,5,6-trichloropicolonic acid), a synthetic auxin, is particularly effective for induction of cell division, proliferation, and further regeneration such as somatic embryogenesis in a range of plant species (Barro et al., 1999;Sanchez-Romero, 2005;Gomes et al., 2006). It acts by inducing auxin sensitivity in nondividing cells that are arrested in G1 (growth phase), enabling them to reenter the S phase (DNA replication) and mitosis (He et al., 2010).Regarding intrinsic factors, genotype is the most determinant in embryogenic competence (Fehér, 2008;Ochatt et al., 2010;Isah, 2016). Various groups, families, and genera are still regarded as recalcitrant to embryogenesis, like Prunus (Druart, 1999). Such recalcitrance has handicapped and delayed the exploitation of biotechnology approaches for breeding in many species and was and still is the subject of research in many laboratories (Ochatt et al., 2010).

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Molecular investigations of the somatic embryogenesis recalcitrancein the cherry (Prunus cerasus L.) rootstock CAB 6P

Mahmoud¹,

Jedidi²,

Delporte³

et al. 2017

Turk J Biol

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“…Additionally, the 18S gene has previously been used in qRT-PCR to normalize the expression of SERK in Theobroma cacao (Santos et al 2005), Lactuca sativa L. (Santos et al 2009), Cocos nucifera L. (Pérez-Núñez et al 2009), Musa spp. (Huang et al 2010), Gossypium hirsutum (Shi et al 2012(Shi et al , 2014, Triticum aestivum L. (Delporte et al 2013), and Curcuma alismatifolia Gagnep (Sucharitakul et al 2014). In the root tissue, the number of CoSERK1 and CoSERK2 transcripts per 18S transcript is 7.87 × 10 −7 (2 −20.2766 ), and 4.32 × 10 −7 (2 −21.1416 ), respectively, this is a proportion of 1:0.55.…”

Section: Discussionmentioning

confidence: 98%

Expression analysis of two SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK) genes during in vitro morphogenesis in Spanish cedar (Cedrela odorata L.)

2018

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Somatic embryogenesis (SE) is one of the most important steps during regeneration, but the molecular mechanism of SE remains unclear for Cedrela odorata. SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) is one of the genes associated with induction of SE and is considered a marker of cells competent to form somatic embryos. Our objective was to clone and characterize the SERK1 and SERK2 gene homologues and analyze their expression patterns during in vitro morphogenesis in Spanish cedar. CoSERK1 and CoSERK2 were isolated from cedar, both share domains characteristic of the SERK family, including leucine-rich repeats, a proline-rich motif, a transmembrane domain, and kinase domains. Embryogenic cultures were established from callus cultures induced on medium supplemented with 1 mg/L dicamba. Histological sections were studied to determine the embryogenic nature of the samples. The CoSERK1 gene was highly expressed during the acquisition of embryogenic competence. The expression level of SERK1 was lower in non-embryogenic tissues and organs than in embryogenic calli, and it was higher in 3-week old embryogenic calli. CoSERK2 gene was highly expressed in leaves and shoots but no difference in expression was obtained between somatic and embryogenic tissues. These results suggest that the expression of CoSERK1 is associated with somatic embryogenesis induction and could be used as a potential marker to monitor the transition from competent to embryogenic cells and tissues in Spanish cedar.

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Role of SERK During Somatic Embryogenesis and Its Interaction with Brassinosteroids

Talapatra

Goswami

Das

et al. 2015

Somatic Embryogenesis in Ornamentals and Its Applications

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Isolation and Expression Analysis of a SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) Gene in Curcuma alismatifolia Gagnep.

Cited by 4 publications

References 27 publications

Molecular investigations of the somatic embryogenesis recalcitrancein the cherry (Prunus cerasus L.) rootstock CAB 6P

Molecular investigations of the somatic embryogenesis recalcitrancein the cherry (Prunus cerasus L.) rootstock CAB 6P

Expression analysis of two SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK) genes during in vitro morphogenesis in Spanish cedar (Cedrela odorata L.)

Role of SERK During Somatic Embryogenesis and Its Interaction with Brassinosteroids

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