Isolation and Functional Characterisation of the Genes Encoding Δ8-Sphingolipid Desaturase from Brassica rapa

Li, Shufen; Song, Liying; Yin, Wei-Bo; Chen, Yuhong; Chen, Liang; Li, Jilin; Wang, Richard R.‐C.; Hu, Zhongliang

doi:10.1016/j.jgg.2011.12.002

Cited by 10 publications

(5 citation statements)

References 51 publications

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“…To further confirm the conservation of amino acid residues in the histidine boxes, the sequence logos of the three histidine boxes in each subfamily were generated using the WebLogo program ( S1 Fig ). As previously reported [ 49 , 50 , 51 ], it was observed that the first residue in the third histidine box was glutamine rather than histidine in the Front-end Desaturase subfamily. Apart from this divergence, the remaining histidines were strongly conserved among subfamilies.…”

Section: Resultssupporting

confidence: 73%

Characterization of 19 Genes Encoding Membrane-Bound Fatty Acid Desaturases and their Expression Profiles in Gossypium raimondii Under Low Temperature

Liu

et al. 2015

PLoS ONE

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To produce unsaturated fatty acids, membrane-bound fatty acid desaturases (FADs) can be exploited to introduce double bonds into the acyl chains of fatty acids. In this study, 19 membrane-bound FAD genes were identified in Gossypium raimondii through database searches and were classified into four different subfamilies based on phylogenetic analysis. All 19 membrane-bound FAD proteins shared three highly conserved histidine boxes, except for GrFAD2.1, which lost the third histidine box in the C-terminal region. In the G. raimondii genome, tandem duplication might have led to the increasing size of the FAD2 cluster in the Omega Desaturase subfamily, whereas segmental duplication appeared to be the dominant mechanism for the expansion of the Sphingolipid and Front-end Desaturase subfamilies. Gene expression analysis showed that seven membrane-bound FAD genes were significantly up-regulated and that five genes were greatly suppressed in G. raimondii leaves exposed to low temperature conditions.

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Section: Resultssupporting

confidence: 73%

Characterization of 19 Genes Encoding Membrane-Bound Fatty Acid Desaturases and their Expression Profiles in Gossypium raimondii Under Low Temperature

Liu

et al. 2015

PLoS ONE

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“…These results indicated that AhSLD-1 , AhSLD-2 , AhSLD-3 , and AhSLD-4 all encoded functional Δ8 sphingolipid desaturases with diverse biochemical functions. In a similar way, four BrSLD1 genes in Brassica rapa have been isolated, which also catalyze different ratios of t18:1 8Z and t18:1 8E [ 73 ]. However, two different genes encoding sphingolipid Δ8 desaturase were discovered in Arabidopsis and Helianthus annuus , there have been no reports on different product ratios in these two species [ 48 , 74 , 75 ].…”

Section: Resultsmentioning

confidence: 99%

Isolation and functional analysis of fatty acid desaturase genes from peanut (Arachis hypogaea L.)

Chi¹,

Zhang²,

Chen³

et al. 2017

PLoS ONE

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BackgroundFatty acid desaturases are enzymes that introduce double bonds into fatty acyl chains. Extensive studies of fatty acid desaturases have been done in many plants. However, less is known about the diversity of this gene family in peanut (Arachis hypogaea L.), an important oilseed crop that is cultivated worldwide.ResultsIn this study, twelve novel AhFADs genes were identified and isolated from peanut. Quantitative real-time PCR analysis indicated that the transcript abundances of AhFAB2-2 and AhFAD3-1 were higher in seeds than in other tissues examined, whereas the AhADS and AhFAD7-1 transcripts were more abundant in leaves. AhFAB2-3, AhFAD3-2, AhFAD4, AhSLD-4, and AhDES genes were highly expressed in flowers, whereas AhFAD7-2, AhSLD-2, and AhSLD-3 were expressed most strongly in stems. During seed development, the expressions of AhFAB2-2, AhFAD3-1, AhFAD7-1, and AhSLD-3 gradually increased in abundance, reached a maximum expression level, and then decreased. The AhFAB2-3, AhFAD3-2, AhFAD4, AhADS, and AhDES transcript levels remained relatively high at the initial stage of seed development, but decreased thereafter. The AhSLD-4 transcript level remained relatively low at the initial stage of seed development, but showed a dramatic increase in abundance at the final stage. The AhFAD7-2 and AhSLD-2 transcript levels remained relatively high at the initial stage of seed development, but then decreased, and finally increased again. The AhFAD transcripts were differentially expressed following exposure to abiotic stresses or abscisic acid. Moreover, the functions of one AhFAD6 and four AhSLD genes were confirmed by heterologous expression in Synechococcus elongates or Saccharomyces cerevisiae.ConclusionsThe present study provides valuable information that improves understanding of the biological roles of FAD genes in fatty acid synthesis, and will help peanut breeders improve the quality of peanut oil via molecular design breeding.

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“…In contrast, yeast ectopic expression studies provided ambiguous results regarding isomer-specific function. Yeast strains expressing plant SLDs show aluminum-resistant phenotypes but there is no apparent correlation between the degree of tolerance and the cis/trans ratio [16,23,32] and thus the functional distinction between LCB cis/trans isomers remains controversial. In addition, it is unknown how sphingolipid classes, with each class comprising various head groups and ceramide backbones, are associated with aluminum toxicity and tolerance due to LCB unsaturation.…”

Section: Introductionmentioning

confidence: 99%

Plant-Unique cis/trans Isomerism of Long-Chain Base Unsaturation is Selectively Required for Aluminum Tolerance Resulting from Glucosylceramide-Dependent Plasma Membrane Fluidity

Sato

Nagano

Jiang

et al. 2019

Plants

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Cis/trans isomerism of the Δ8 unsaturation of long-chain base (LCB) is found only in plant sphingolipids. This unique geometry is generated by sphingolipid LCB Δ8 desaturase SLD which produces both isomers at various ratios, resulting in diverse cis/trans ratios in plants. However, the biological significance of this isomeric diversity remains controversial. Here, we show that the plant-specific cis unsaturation of LCB selectively contributes to glucosylceramide (GlcCer)-dependent tolerance to aluminum toxicity. We established three transgenic rice lines with altered LCB unsaturation profiles. Overexpression of SLD from rice (OsSLD-OX), which preferentially exhibits cis-activity, or Arabidopsis (AtSLD-OX), showing preference for trans-activity, facilitated Δ8 unsaturation in different manners: a slight increase of cis-unsaturated glycosylinositolphosphoceramide (GIPC) in OsSLD-OX, and a drastic increase of trans-unsaturated GlcCer and GIPC in AtSLD-OX. Disruption of LCB Δ4 desaturase (des) significantly decreased the content of GlcCer. Fluorescence imaging analysis revealed that OsSLD-OX and AtSLD-OX showed increased plasma membrane fluidity, whereas des had less fluidity, demonstrating that the isomers universally contributed to increasing membrane fluidity. However, the results of a hydroponic assay showed decreased aluminum tolerance in AtSLD-OX and des compared to OsSLD-OX and the control plants, which did not correlate with membrane fluidity. These results suggest that cis-unsaturated GlcCer, not GIPC, selectively serves to maintain the membrane fluidity specifically associated with aluminum tolerance.

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Isolation and Functional Characterisation of the Genes Encoding Δ8-Sphingolipid Desaturase from Brassica rapa

Cited by 10 publications

References 51 publications

Characterization of 19 Genes Encoding Membrane-Bound Fatty Acid Desaturases and their Expression Profiles in Gossypium raimondii Under Low Temperature

Characterization of 19 Genes Encoding Membrane-Bound Fatty Acid Desaturases and their Expression Profiles in Gossypium raimondii Under Low Temperature

Isolation and functional analysis of fatty acid desaturase genes from peanut (Arachis hypogaea L.)

Plant-Unique cis/trans Isomerism of Long-Chain Base Unsaturation is Selectively Required for Aluminum Tolerance Resulting from Glucosylceramide-Dependent Plasma Membrane Fluidity

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