Isolation and functional characterization of MxCS1: a gene encoding a citrate synthase in Malus xiaojinensis

Han, Deguo; Wang, Y.; Zhang, L.; Ma, Ligeng; Zhang, X. Z.; Xu, Xiangyu; Han, Zhenhai

doi:10.1007/s10535-012-0015-4

Cited by 27 publications

(13 citation statements)

References 22 publications

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“…This is in line with the use of citric acid in industry for cleaning and prevention of the clogging of pipes with colloidal and particulate Fe. An increased activity of citrate synthase (CS) and/or an overexpression of CS genes has been reported in plants grown with low Fe supply (e.g., Beta vulgaris López-Millán et al, 2000, Pyrus communis López-Millán et al, 2001, A. thaliana Thimm et al, 2001, Actinidia deliciosa Rombolà et al, 2002, S. lycopersicum López-Millán et al, 2009, Pisum sativum Jelali et al, 2010, Malus xiaojinensis Han et al, 2012 and citrus Martínez-Cuenca et al, 2013) and more recently in M. xiaojinensis plants grown with excessive Fe supply (Han et al, 2012). In fact, overexpression of an apple CS gene increased tolerance to Fe stress (low and excessive Fe supply) in transgenic A. thaliana and Nicotiana tabacum plants (Han et al, 2012, 2013).…”

Section: Ligands In Plant Fluidsmentioning

confidence: 99%

“…An increased activity of citrate synthase (CS) and/or an overexpression of CS genes has been reported in plants grown with low Fe supply (e.g., Beta vulgaris López-Millán et al, 2000, Pyrus communis López-Millán et al, 2001, A. thaliana Thimm et al, 2001, Actinidia deliciosa Rombolà et al, 2002, S. lycopersicum López-Millán et al, 2009, Pisum sativum Jelali et al, 2010, Malus xiaojinensis Han et al, 2012 and citrus Martínez-Cuenca et al, 2013) and more recently in M. xiaojinensis plants grown with excessive Fe supply (Han et al, 2012). In fact, overexpression of an apple CS gene increased tolerance to Fe stress (low and excessive Fe supply) in transgenic A. thaliana and Nicotiana tabacum plants (Han et al, 2012, 2013). Increases in xylem carboxylate concentrations have also been described with other metal stresses, including excess of metals in crop (e.g., Zn in sugar beet Sagardoy et al, 2011 and Cd in tomato Sagardoy, 2012), and forest species (Cd in Quercus suber Gogorcena et al, 2011), as well as in metal hyper-accumulators (e.g., the Zn hyper-accumulator T. caerulescens Lasat et al, 1998, the Cd hyper-accumulator genotype of B. juncea L. Wei et al, 2007a and the Ni hyper-accumulator tree S. acuminata Callahan et al, 2008).…”

Section: Ligands In Plant Fluidsmentioning

confidence: 99%

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Metal species involved in long distance metal transport in plants

Álvarez‐Fernández¹,

Díaz-Benito²,

Abadı́a³

et al. 2014

Front. Plant Sci.

129

106

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The mechanisms plants use to transport metals from roots to shoots are not completely understood. It has long been proposed that organic molecules participate in metal translocation within the plant. However, until recently the identity of the complexes involved in the long-distance transport of metals could only be inferred by using indirect methods, such as analyzing separately the concentrations of metals and putative ligands and then using in silico chemical speciation software to predict metal species. Molecular biology approaches also have provided a breadth of information about putative metal ligands and metal complexes occurring in plant fluids. The new advances in analytical techniques based on mass spectrometry and the increased use of synchrotron X-ray spectroscopy have allowed for the identification of some metal-ligand species in plant fluids such as the xylem and phloem saps. Also, some proteins present in plant fluids can bind metals and a few studies have explored this possibility. This study reviews the analytical challenges researchers have to face to understand long-distance metal transport in plants as well as the recent advances in the identification of the ligand and metal-ligand complexes in plant fluids.

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Section: Ligands In Plant Fluidsmentioning

confidence: 99%

Section: Ligands In Plant Fluidsmentioning

confidence: 99%

Metal species involved in long distance metal transport in plants

Álvarez‐Fernández¹,

Díaz-Benito²,

Abadı́a³

et al. 2014

Front. Plant Sci.

129

106

View full text Add to dashboard Cite

show abstract

“…An increase in MxNAS1 levels could induce a higher rate of synthesis of NAS and NA; moreover, NA promotes Fe absorption from the environment (Han et al . ). Studies on M. xiaojinensis have shown that it can efficiently tolerate Fe deficiency.…”

Section: Discussionmentioning

confidence: 97%

“…zinc finger proteins; Han et al . ). Iron deficiency can induce Nas1 expression and increase NA content in both Strategy I (Zuchi et al .…”

Section: Introductionmentioning

confidence: 97%

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Iron deficiency stress can induce MxNAS1 protein expression to facilitate iron redistribution in Malus xiaojinensis

et al. 2017

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Iron (Fe) is a vital trace element in plants, and deficiency of this element in apple trees can reduce fruit quality. Nicotianamine (NA) is known to play an important role in Fe transport and endogenous hormone balance. In the present study, we investigated the role of a nicotianamine synthase 1 gene (MxNas1) in an apple species, Malus xiaojinensis, that has a more Fe-efficient genotype than other apple species and ecotypes. To characterise the response of M. xiaojinensis to Fe deficiency, we used quantitative Q-PCR to determine the level of expression of MxNas1 and Western blot to measure protein levels. Immunohistochemical staining and GFP fluorescence localisation of the MxNAS1 protein were also carried out. HPLC and polarised absorption spectrophotometry were performed to investigate the effects of overexpression of MxNas1 in order to elucidate the role of MxNAS1 in the cellular uptake of active Fe in tobacco suspension cells. We found that MxNas1 expression and protein levels were higher under Fe deficiency stress than under Fe sufficiency. Immunohistochemical staining showed that MxNAS1 was localised mainly in the epidermal and vascular tissues of the roots, vascular tissues of the stem and palisade cells of mature leaves, and in parenchyma cells of young leaves. MxNAS1 was mainly localised in the plasma membranes and vesicles of protoplasts. In addition, overexpression of MxNas1 in stable transgenic tobacco cells increased NA and active Fe content under Fe sufficiency. The results suggest that MxNas1 expression in M. xiaojinensis is induced in response to Fe deficiency stress, resulting in higher levels of the protein. MxNAS1 may be involved in the redistribution of Fe in M. xiaojinensis under Fe deficiency.

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Low irradiance alters carbon metabolism and delays flower stalk development in two orchids

Liu²,

Tseng³

et al. 2013

Biologia plant.

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In Phalaenopsis, lowering irradiance has been used to delay flower stalk development but the accompanying biochemical changes remain poorly understood. We cultured two commercial Phalaenopsis-type orchids, Phalaenopsis cv. Sogo Yukidian V3, and Doritaneopsis cv. Walnut Valley Halo ES09 under reduced irradiance by under-bench shading (approximately 15 % of mean control irradiance) for 15 weeks in a greenhouse under the natural photoperiod. Besides delaying flower stalk development as expected, the treatment greatly decreased the activities of ribulose-1,5bisphosphate carboxylase/oxygenase, phosphoenolpyruvate carboxylase, and NAD + -malic enzyme, and reduced the nocturnal malate accumulation and daytime starch deposition, the typical diurnal metabolite fluctuations of crassulacean acid metabolism (CAM) plants. As well, the content of sucrose and starch was reduced at dawn and dusk whereas the content of glucose and fructose only at dawn. The persistent decrease in the sucrose content under shading may be an inhibitory signal of flower stalk induction.

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Isolation and functional characterization of MxCS1: a gene encoding a citrate synthase in Malus xiaojinensis

Cited by 27 publications

References 22 publications

Metal species involved in long distance metal transport in plants

Metal species involved in long distance metal transport in plants

Iron deficiency stress can induce MxNAS1 protein expression to facilitate iron redistribution in Malus xiaojinensis

Low irradiance alters carbon metabolism and delays flower stalk development in two orchids

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