Isolation and genetic analysis of BoHV-1 gC gene isolated from animals suffering respiratory disease in Dakahlia, Egypt 2018

Abstract

The aim of the present study was to isolate, identify and genetically characterize bovine herpes virus Type 1 (BoHV-1) from suspected cattle showing clinical symptoms of respiratory disease in Dakahlia, Egypt, September 2018. BoHV-1 was isolated on specific pathogen freeembryonated chicken eggs (SPF-ECE) from suspected nasal swabs and lung tissue samples. Pock lesions on the chorio-allantoic membrane (CAM) of SPF-ECE ranged from 1 to 2 mm in diameter and scattered all over the membrane that were more pronounced after the third passage. PCR assay specific for BoHV-1 gC gene was also performed for molecular identification of the viral isolate and the gC PCR product was sequenced. Phylogenetic analysis of gC gene sequence identified and clustered our isolate with BoHV1. This highlights the isolation, rapid and sensitive detection, and identification of BoHV-1 virus from clinical cases and its subtyping by nucleotide sequencing and subsequent phylogenetic analysis which gives valuable information about the molecular epidemiology of BoHV-1 subtypes prevalent in the country