Isolation and genetic characterizaion of Escherichia coli K-12 mutations affecting bacteriophage T5 restriction by the ColIb plasmid

Hull, Richard A.; Moody, E. E. M.

doi:10.1128/jb.127.1.229-236.1976

Cited by 13 publications

(6 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Finally, if the internal cellular targets for colicin Ib, which are coded by the host genome, are altered, the internal colicin Ib that is activated because the immunity protein is inactivated cannot kill the cells because either the colicin Ib cannot recognize the cellular targets or, if they are recognized, they no longer transmit the influence of the colicin Ib to other molecules that bring about deterioration and death of the cell. This is what is shown by cmr mutants of the host (15).…”

Section: Discussionsupporting

confidence: 80%

Is abortive infection by bacteriophage BF23 of Escherichia coli harboring ColIb plasmids due to cell killing by internally liberated colicin Ib?

McCorquodale¹,

Shaw²,

Moody³

et al. 1979

J Virol

Self Cite

View full text Add to dashboard Cite

Infection of Escherichia coli harboring ColIb+ plasmids with bacteriophage BF23+ is abortive and resulted in changes of membrane permeability as measured by efflux of nucleotides and K+. A single pre-early gene product of BF23+ was necessary and sufficient to elicit the abortive response. Appropriate mutations in this pre-early gene allowed a productive infection in ColIb+ cells. Appropriate mutations in the ColIb plasmid also allowed a productive infection with BF23+. A comparison of changes occurring during abortive infection and during killing of sensitive cells by external colicin lb or Ia, together with certain genetic data, has led to the conclusion that membrane changes accompanying the two phenomena are the result of a common mechanism, namely, the interaction of free colicin with the cytoplasmic membrane. Bacteriophage BF23 and its close relative bacteriophage T5 do not productively infect hosts that carry ColIb plasmids (31). Adsorption of phage particles and transfer of phage DNA proceed normally in such infections, but soon after the pre-early genes, which comprise the initially transferred 8% segment of DNA, are expressed, further gene expression ceases (24) despite the persisting intactness of the phage DNA in the cell (31). This sequence of events has led to the suggestion that a ColIb-specified product interferes with transcription of phage DNA (25, 32). However, the occurrence of early abortive lysis in such infections (27) suggests that changes more severe than arrest of transcription are occurring in the infected cells. We reported preliminary data at the phage meetings in Cold Spring Harbor, N.Y., during 1974 (A

show abstract

Section: Discussionsupporting

confidence: 80%

Is abortive infection by bacteriophage BF23 of Escherichia coli harboring ColIb plasmids due to cell killing by internally liberated colicin Ib?

McCorquodale¹,

Shaw²,

Moody³

et al. 1979

J Virol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Blood agar plates were made with Blood Agar Base (BBL Microbiology Systems) supplemented with 0.5% yeast extract-0.1% glucose-10 mM CaCI-5% defibrinated sheep blood. Minimal salts media and the concentrations of various growth supplements used have been described elsewhere (15). Buffered saline (pH 7) contained (per liter) 8.5 g of NaCl, 0.3 g of KH2PO4, and 1.22 g of Na2HPO4 7H2O.…”

mentioning

confidence: 99%

Genetics of hemolysin of Escherichia coli

Hull¹,

Hull²,

Minshew³

et al. 1982

J Bacteriol

View full text Add to dashboard Cite

The expression of alpha-hemolysin is a property frequently associated with Escherichia coli extraintestinal infections. We have examined the genetic basis for hemolysin expression by an E. coli strain isolated from a human urinary tract infection. The genes necessary for hemolysin synthesis were found to be chromosomal and to map near the ilv gene cluster. Isogenic hly+ and hly derivatives were also prepared and tested for virulence in the chicken embryo model system. Hemolysin was found to be necessary but not in itself sufficient for E. coli virulence in this in vitro model.

show abstract

“…Likewise, colicinogenic strains that are permissive hosts for T5 wild-type phage have been found. These bacteria allow T5 replication because of chromosomal mutations (23) or ColIb plasmid mutations (28; Richard Moyer, personal communication). Thus, the interaction of at least three proteins (one specified by the host, one specified by the phage, and one specified by the plasmid) is necessary for abortive infection.…”

mentioning

confidence: 99%

Amino acid and sugar transport in Escherichia coli (ColIb) during abortive infection by bacteriophage T5

Glenn¹,

Duckworth²

1979

J Virol

View full text Add to dashboard Cite

T5 bacteriophage cannot replicate in Escherichia coli containing the colicinogenic factor ColIb. We show that active transport of proline and glutamine begins to decline at about 10 min after infection, the same time at which macromolecular synthesis stops during abortive infection. Uptake of a-methylglucoside is stimulated, however, and this change is evident even by 5 min after infection. These changes in membrane function do not occtir during infections that are productive because of mutations on the plasmid or phage. The results suggest that the abortive infection is caused by membrane depolarization.a Measured by killing of E. coli B and C. b ColIR indicates resistance to the external action of colicin lb. c ColIb hindicates the mutant ColIb P9 factor, which allows T5 growth.

show abstract

Isolation and genetic characterizaion of Escherichia coli K-12 mutations affecting bacteriophage T5 restriction by the ColIb plasmid

Cited by 13 publications

References 30 publications

Is abortive infection by bacteriophage BF23 of Escherichia coli harboring ColIb plasmids due to cell killing by internally liberated colicin Ib?

Is abortive infection by bacteriophage BF23 of Escherichia coli harboring ColIb plasmids due to cell killing by internally liberated colicin Ib?

Genetics of hemolysin of Escherichia coli

Amino acid and sugar transport in Escherichia coli (ColIb) during abortive infection by bacteriophage T5

Contact Info

Product

Resources

About