Isolation and genetic characterization of Erwinia amylovora bacteria from Kyrgyzstan

Döölotkeldieva, Tinatin; Bobushova, Saikal; Schuster, Christina; Konurbaeva, Mahabat; Leclerque, Andreas

doi:10.1007/s10658-019-01790-3

Cited by 13 publications

(8 citation statements)

References 34 publications

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“…This approach was then implemented with the isolates of the 2018-2019 sampling campaigns to infer the genotypes present in Southern Kyrgyzstan, within the Arslonbob walnut-fruit forests and around Lake Issyk-Kul in the north of the country. For all isolates retrieved in the Issyk-Kul region the PCR assays yielded amplicon sizes compatible with genotypes (A, t, α) (Table 3), which confirmed the results previously obtained using Sanger sequencing for KTMU isolates that demonstrated the deletion of spacer 2016 in CCR2 (Doolotkeldieva et al 2019) (Fig. 3).…”

Section: Discussionsupporting

confidence: 86%

“…All isolates from the locations (#27-#29, Table 1) surrounding lake Issyk-Kul (four from 2018 and one, IS-ZH-19, from 2019) displayed PCR results compatible with genotype (A, t, α). For the 2018 isolates this is also confirmed by sequencing data obtained previously (Doolotkeldieva et al 2019). Among the other locations investigated, results compatible with genotype (A, t, α) were found only for isolate JA-19-19, which was recovered from P. communis in the Aral village (Jalal-Abad region) near the border to Uzbekistan.…”

Section: Sequencing Of Crispr Regions From Selected 2012-2013 Isolatessupporting

confidence: 80%

“…Symptomatic plant material was collected in late summer 2018 from disease-infested orchards in three locations around lake Issyk-Kul, one of the most important fruit production regions of Kyrgyzstan, yielding the five KTMU isolates (Table 3), which were partially characterized earlier (Doolotkeldieva et al 2019). From location #27 (Jeti Oguz District, Saruu village), a further positive sample yielding isolate IS-ZH-19 was obtained in 2019.…”

Section: Symptom Detectionmentioning

confidence: 99%

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Genetic characterization of Erwinia amylovora isolates detected in the wild walnut-fruit forest of South Kyrgyzstan

et al. 2021

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Erwinia amylovora, the causal agent of fire blight, was first reported in Kyrgyz orchards in 2008 and, within a few years, the disease has spread throughout most of the northern part of the country. As fire blight expanded and penetrated new areas, it also reached the unique ecosystem constituted by the Arslonbob wild walnut-fruit forests in South Kyrgyzstan. Here, we present the results of field surveys in the forests and in the nearby orchards and gardens of the Jalal-Abad region during the 2019 season. Further, we use genetic profiling of the CRISPR regions to compare the genotypes of the obtained isolates to those of previous outbreaks in the north of the country and in nearby Kazakhstan. While fire blight was found in twelve of the twenty-six sites investigated in and around the Arslonbob walnut-fruit forest, it was mostly located on domesticated varieties of quince, apple or pear in gardens, orchards or nurseries. Plants in the forest and especially endangered wild species were not affected by the disease, but their proximity to the current infection herds is cause of major concern and calls for immediate protective action. Genetic analysis suggests that the main introduction route for fire blight to Central Asia was import of contaminated plant material from already affected countries in the Eurasian continent, which probably occurred on multiple independent instances. Nonetheless, indications of natural spread of the disease were identified between the regions of South Kazakhstan and North Kyrgyzstan.

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Section: Discussionsupporting

confidence: 86%

Section: Sequencing Of Crispr Regions From Selected 2012-2013 Isolatessupporting

confidence: 80%

Section: Symptom Detectionmentioning

confidence: 99%

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Genetic characterization of Erwinia amylovora isolates detected in the wild walnut-fruit forest of South Kyrgyzstan

et al. 2021

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“…The bacterial cultures used in this work were isolated from diseased organs of apple and pear trees grown in orchards and forestry farms in northern and southern Kyrgyzstan. They were genetically identified, and their degree of pathogenicity was determined [37,38].…”

Section: Effects Of Nps On Plant Pathogensmentioning

confidence: 99%

Biological Activity of Ag and Cu Monometallic Nanoparticles and Ag‐Cu Bimetallic Nanocomposites against Plant Pathogens and Seeds

Döölotkeldieva

Bobusheva

Zhasnakunov

et al. 2022

Journal of Nanomaterials

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The antibacterial activities of silver (Ag) and copper (Cu) monometallic and bimetallic nanoparticles (MNPs and BNPs) against the most economically important and common plant bacterial diseases in Kyrgyzstan were tested, as well as their phytotoxic effects on wheat and corn seeds. To achieve this, a range of Ag and Cu MNPs and BNPs were synthesized using different methods and were tested at different concentrations to compare their biological effects. The antibacterial activities of Ag and Cu MNPs and Ag-Cu BNPs against the plant pathogenic bacteria Erwinia amylovora, Pseudomonas syringae, and Pectobacterium carotovorum were evaluated. Of the three pathogens, Ps. syringae was found to be the most sensitive to NPs. The antimicrobial activities of the tested NPs showed significant positive correlations with the concentration; hence, there was a dose-dependent effect. Due to their antibacterial properties, these Ag-Cu nanocomposites have high potential in plant protection applications.

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“…The etiological agent responsible for this disease is the quarantine bacterium Erwinia amylovora (Burril) Winslow et al, a Gram-negative of the Erwiniaceae family, which has been considered as one of the top ten plant pathogenic bacteria [4,5]. This pathogen was first reported in North America, but is currently spread to Europe (United Kingdom, Spain, France, Italy, Hungary, and Portugal), South Korea, New Zealand, and the Middle East [6][7][8][9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Evaluation of Five Antimicrobial Peptides against the Plant Pathogen Erwinia amylovora

et al. 2021

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Fire blight is a major pome fruit trees disease that is caused by the quarantine phytopathogenic Erwinia amylovora, leading to major losses, namely, in pear and apple productions. Nevertheless, no effective sustainable control treatments and measures have yet been disclosed. In that regard, antimicrobial peptides (AMPs) have been proposed as an alternative biomolecule against pathogens but some of those AMPs have yet to be tested against E. amylovora. In this study, the potential of five AMPs (RW-BP100, CA-M, 3.1, D4E1, and Dhvar-5) together with BP100, were assessed to control E. amylovora. Antibiograms, minimal inhibitory, and bactericidal concentrations (minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC), growth and IC50 were determined and membrane permeabilization capacity was evaluated by flow cytometry analysis and colony-forming units (CFUs) plate counting. For the tested AMPs, the higher inhibitory and bactericidal capacity was observed for RW-BP100 and CA-M (5 and 5–8 µM, respectively for both MIC and MBC), whilst for IC50 RW-BP100 presented higher efficiency (2.8 to 3.5 µM). Growth curves for the first concentrations bellow MIC showed that these AMPs delayed E. amylovora growth. Flow cytometry disclosed faster membrane permeabilization for CA-M. These results highlight the potential of RW-BP100 and CA-M AMPs as sustainable control measures against E. amylovora.

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Isolation and genetic characterization of Erwinia amylovora bacteria from Kyrgyzstan

Cited by 13 publications

References 34 publications

Genetic characterization of Erwinia amylovora isolates detected in the wild walnut-fruit forest of South Kyrgyzstan

Genetic characterization of Erwinia amylovora isolates detected in the wild walnut-fruit forest of South Kyrgyzstan

Biological Activity of Ag and Cu Monometallic Nanoparticles and Ag‐Cu Bimetallic Nanocomposites against Plant Pathogens and Seeds

In Vitro Evaluation of Five Antimicrobial Peptides against the Plant Pathogen Erwinia amylovora

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