Isolation and genetic characterization of bovine ephemeral fever virus from epidemic-2020 in Turkey

Karayel-Hacioglu, Ilke; Yelken, Selda Duran; Vezir, Yaser; Ünal, N.; Alkan, Feray

doi:10.1007/s11250-021-02715-1

Cited by 4 publications

(3 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was determined that the BEF viruses circulat-ing during the 2020 outbreak in Turkey belonged to the Middle East lineage (Fig. 3), which is also in line with the results of another study regarding the same outbreak (Karayel-Hacioglu et al, 2021).…”

Section: Discussionsupporting

confidence: 87%

“…Serological studies of BEFV in Turkey, the number of studies regarding the molecular identification and typing of BEFV is very limited (Tonbak et al, 2013;Oguzoglu et al, 2015;Abayli et al, 2017;Alkan et al, 2017;Karayel-Hacioglu et al, 2021). Sera samples collected from 10 cattle from Sakarya, 20 from Adıyaman and 26 from Adana, 56 cattle in total, during the 2012 BEF outbreak in Turkey were examined with RT-PCR, and 85.71% (48/56) positivity was detected.…”

Section: Discussionmentioning

confidence: 99%

“…The phylogenetic analysis of the virus isolated during the 2012 outbreak in Turkey revealed that both the Middle East and Eastern Asia lineages simultaneously triggered an outbreak (Erganiş et al, 2013, Tonbak et al, 2013, Oguzoglu et al, 2015. The researchers (Karayel-Hacioglu et al, 2021) reported that 2020 isolates belonged to the Middle East lineage based on the phylogenetic analysis of two positive samples.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Prevalence, isolation and molecular characterization of Bovine Ephemeral Fever Virus in south and southeast regions of Turkey in the outbreak of 2020

Tokgoz,

Sözmen

et al. 2024

J Hellenic Vet Med Soc

View full text Add to dashboard Cite

The aim of this study is to investigate the presence of BEF disease in the blood, sera and tissue samples sent to the Viral Diagnosis Laboratory of Adana Veterinary Control Institute with suspected BEF in 2020 from different provinces of Turkey as well as to perform characterization and phylogenetic analysis of cases tested positive with molecular methods. For this purpose, 79 defibrinated blood and 2 tissue samples as well as 168 sera samples obtained from cattle varying in breed, age, and sex, were examined by using Real Time RT-PCR and Blocking ELISA, respectively. Two new degenerate primers amplifying 956 bp of the protein G (AVKEF_AATGTTCCNGTGAATTGTGGAG and AVKER_TGCATAATCYCTTCCTGGTCT) were designed and the phylogenetic analysis of the samples tested positive with RT-PCR was performed. As a result of the examinations conducted, 64.20% (52/81) of the defibrinated blood and tissue samples, and 69.04% (116/168) of the sera samples were diagnosed as positive. Through VERO cell culture, the virus was isolated from the tissue samples of 2 animals in Adana and Sanliurfa provinces, which were positive. The phylogenetic analysis revealed that the virus circulated in Turkey during the 2020 pandemic belongs to the Middle East lineage. We concluded that monitoring the disease and identifying the lineages of the virus that have caused pandemic are significant in the selection of the proper vaccine and the disease control.

show abstract

Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Prevalence, isolation and molecular characterization of Bovine Ephemeral Fever Virus in south and southeast regions of Turkey in the outbreak of 2020

Tokgoz,

Sözmen

et al. 2024

J Hellenic Vet Med Soc

View full text Add to dashboard Cite

show abstract

Epidemiology, Genetic Characterization, and Disease of Bovine Ephemeral Fever Virus (BEFV)

Lekcharoensuk,

Chaisirirat

2024

Veterinary Virology of Domestic and Pet Animals

View full text Add to dashboard Cite

Molecular characterization and phylogenetic analysis of bovine ephemeral fever viruses in Khuzestan province of Iran in 2018 and 2020

Rezatofighi

Mirzadeh²,

Mahmoodi

2022

BMC Vet Res

View full text Add to dashboard Cite

Background Bovine ephemeral fever (BEF) is an arthropod-borne viral disease caused by the BEF virus (BEFV). This single-stranded RNA virus that affects cattle and water buffalo is endemic in tropical and subtropical regions including Iran. While BEF is a major disease of cattle in Iran, information regarding its agent, molecular characterization, and circulating viruses are highly limited. The current study aimed to, firstly, determine the genetic and antigenic characteristics of BEFV strains in Khuzestan province in Southwest of Iran in 2018 and 2020 and, secondly, to compare them with strains obtained from other areas. Results By phylogenetic analysis based on the Glycoprotein gene, BEFV strains were divided into four clusters of Middle East, East Asia, South Africa, and Australia; in which the 2018 and 2020 Iranian BEFV strains were grouped in the Middle East cluster with the Turkish, Indian, and Israeli strains. Depending on the chronology and geographical area, the outbreaks of Turkey (2020), Iran (2018 and 2020), and India (2018 and 2019) are proposed to be related. These BEFVs had the highest identity matrix and the lowest evolutionary distance among the studied strains. Multiple sequence alignment of G1, G2, and G3 antigenic sites showed that these neutralizing epitopes are highly conserved among the strains of the Middle East cluster; however, the strains previously identified in Iran differed in three amino acids placed in G1 and G2 epitopes. Conclusion The findings revealed that BEFVs circulating in the Middle East are closely related phylogenetically and geographically. They also have similar antigenic structures; therefore, developing a vaccine based on these strains can be effective for controlling BEF in the Middle East.

show abstract

Isolation and genetic characterization of bovine ephemeral fever virus from epidemic-2020 in Turkey

Cited by 4 publications

References 21 publications

Prevalence, isolation and molecular characterization of Bovine Ephemeral Fever Virus in south and southeast regions of Turkey in the outbreak of 2020

Prevalence, isolation and molecular characterization of Bovine Ephemeral Fever Virus in south and southeast regions of Turkey in the outbreak of 2020

Epidemiology, Genetic Characterization, and Disease of Bovine Ephemeral Fever Virus (BEFV)

Molecular characterization and phylogenetic analysis of bovine ephemeral fever viruses in Khuzestan province of Iran in 2018 and 2020

Contact Info

Product

Resources

About