Isolation and Genetic Characterization of pGKL Killer-insensitive Mutants<i>(iki)</i>from<i>Saccharomyces cerevisiae</i>

Kishida, Masao; Tokunaga, Masao; Katayose, Yūichi; Yajima, H.; Kawamura-Watabe, Akiko; Hishinuma, Fumio

doi:10.1271/bbb.60.798

Cited by 61 publications

(67 citation statements)

References 5 publications

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“…Two other proteins identified in our study that affect sulfide production, Sit4p and Iki3p, are also known to form a complex with Hht2p (15,23,37). SIT4 is involved in transcriptional regulation (9), and IKI3 encodes for a subunit of the elongator complex (7,22,23). The fact that deletion of all three of these genes leads to increased H 2 S suggests that the reported interaction of their gene products is of biological significance and this complex exerts some form of regulation on the reduction of sulfide, either directly or indirectly.…”

Section: Discussionmentioning

confidence: 77%

Identification of Genes Affecting Hydrogen Sulfide Formation inSaccharomyces cerevisiae

Linderholm

Findleton

Kumar

et al. 2008

Appl Environ Microbiol

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A screen of the Saccharomyces cerevisiae deletion strain set was performed to identify genes affecting hydrogen sulfide (H 2 S) production. Mutants were screened using two assays: colony color on BiGGY agar, which detects the basal level of sulfite reductase activity, and production of H 2 S in a synthetic juice medium using lead acetate detection of free sulfide in the headspace. A total of 88 mutants produced darker colony colors than the parental strain, and 4 produced colonies significantly lighter in color. There was no correlation between the appearance of a dark colony color on BiGGY agar and H 2 S production in synthetic juice media. Sixteen null mutations were identified as leading to the production of increased levels of H 2 S in synthetic juice using the headspace analysis assay. All 16 mutants also produced H 2 S in actual juices. Five of these genes encode proteins involved in sulfur containing amino acid or precursor biosynthesis and are directly associated with the sulfate assimilation pathway. The remaining genes encode proteins involved in a variety of cellular activities, including cell membrane integrity, cell energy regulation and balance, or other metabolic functions. The levels of hydrogen sulfide production of each of the 16 strains varied in response to nutritional conditions. In most cases, creation of multiple deletions of the 16 mutations in the same strain did not lead to a further increase in H 2 S production, instead often resulting in decreased levels.

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Section: Discussionmentioning

confidence: 77%

Identification of Genes Affecting Hydrogen Sulfide Formation inSaccharomyces cerevisiae

Linderholm

Findleton

Kumar

et al. 2008

Appl Environ Microbiol

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show abstract

“…[34][35][36][37] Certain strains of the dairy yeast K. lactis contains "killer DNA," a plasmid pair (k1 and k2) encoding a 3-subunit anti-yeast toxin complex known as zymocin. [38][39][40] Upon secretion, the a-and b-subunits dock the zymocin to the cell wall of susceptible yeasts and facilitate transfer of the cytotoxic g-subunit, which will arrest the cell before START in the G1 phase of the cell cycle.…”

Section: Kluveromyces Lactis G-toxin a Tool To Identify Genes Requirementioning

confidence: 99%

“…[34][35][36][37] Strains with mutations in any of the Elongator subunit genes (ELP1-ELP6), killer toxin insensitive genes (KTI11-KTI14), the TRM9 gene, the SIT4 gene or both the SAP185 and SAP190 genes are type II mutants [34][35][36][37]40,45 , and these mutants are unable to form mcm 5 and ncm 5 side chains at wobble position ( Table 2). 17,46 To identify additional mutants affecting formation of the mcm 5 group, a yeast deletion collection containing 4800 strains, with different non-essential gene deletions, was screened for resistance to zymocin.…”

Section: 43mentioning

confidence: 99%

“…The SUP4-encoded ochre suppressor tRNA, where the primary anticodon sequence is UUA, has a mcm 5 side chain at U 34 . 17 In an elp3 mutant lacking the entire mcm 5 side chain resulting in an unmodified U 34 , efficiency of decoding the cognate ochre UAA and the near cognate amber UAG codons by the SUP4 suppressor tRNA is reduced. 73 Also tRNA Arg mcm 5 UCU having the anticodon sequence UCU has a mcm 5 side chain at U 34 .…”

Section: -73mentioning

confidence: 99%

“…17 In an elp3 mutant lacking the entire mcm 5 side chain resulting in an unmodified U 34 , efficiency of decoding the cognate ochre UAA and the near cognate amber UAG codons by the SUP4 suppressor tRNA is reduced. 73 Also tRNA Arg mcm 5 UCU having the anticodon sequence UCU has a mcm 5 side chain at U 34 . In a trm9 mutant lacking the methyl esterification of cm 5 U 34 , efficiency of decoding of multiple cognate AGA and multiple near cognate AGG codons by tRNA Arg cm 5 UCU is decreased.…”

Section: -73mentioning

confidence: 99%

See 2 more Smart Citations

Elongator, a conserved complex required for wobble uridine modifications in Eukaryotes

Karlsborn

Tükenmez

Mahmud³

et al. 2014

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Structural asymmetry in the eukaryotic Elongator complex

et al. 2017

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Edited by Wilhelm JustNucleoside modifications in tRNA anticodons regulate ribosome dynamics during translation elongation and, thereby, fine-tune global protein synthesis rates. The highly conserved eukaryotic Elongator complex conducts specific C5-substitutions in tRNA wobble base uridines. It harbors two copies of each of its six individual subunits, which are all equally important for its activity. Here, we summarize recent developments focusing on the architecture of the Elongator complex, showing an asymmetric subunit arrangement, and its functional implications. In addition, we discuss the role of its proposed active site, its individual subunits and temporarily associated regulatory factors. Finally, we aim to provide mechanistic explanations for the link between mutations in Elongator subunits and the onset of several severe human pathologies.

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Isolation and Genetic Characterization of pGKL Killer-insensitive Mutants(iki)fromSaccharomyces cerevisiae

Cited by 61 publications

References 5 publications

Identification of Genes Affecting Hydrogen Sulfide Formation inSaccharomyces cerevisiae

Identification of Genes Affecting Hydrogen Sulfide Formation inSaccharomyces cerevisiae

Elongator, a conserved complex required for wobble uridine modifications in Eukaryotes

Structural asymmetry in the eukaryotic Elongator complex

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