Isolation and genetic characterization of Neospora caninum from naturally infected sheep

García-Sánchez, Marta; Moreno-Gonzalo, Javier; González-Warleta, Marta; Mezo, Mercedes; Ortega‐Mora, Luis Miguel; Regidor‐Cerrillo, Javier

doi:10.1016/j.vetpar.2020.109091

Cited by 6 publications

(3 citation statements)

References 20 publications

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“…In the same way, the identification of a single genotype by microsatellite genotyping suggests the clonal propagation of N. caninum in the flock and the entry of disease in the flock from a unique source of infection. The genotype found is very similar to that described in another sheep flock in Spain [8,9,49], with variations only in MS4, MS5, and MS21 markers.…”

Section: Discussionsupporting

confidence: 83%

Dynamics of Neospora caninum-Associated Abortions in a Dairy Sheep Flock and Results of a Test-and-Cull Control Programme

et al. 2021

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Neospora caninum is an apicomplexan parasite that can cause abortions and perinatal mortality in sheep. Although ovine neosporosis has been described worldwide, there is a lack of information about the relationship between N. caninum serostatus and the reproductive performance. In this study, we described the infection dynamics in a dairy sheep flock with an abortion rate up to 25% and a N. caninum seroprevalence of 32%. Abortions were recorded in 36% and 9% of seropositive and seronegative sheep, respectively. Seropositive sheep were more likely to abort twice (OR = 4.44) or three or more times (OR = 10.13) than seronegative sheep. Endogenous transplacental transmission was the main route of transmission since 86% of seropositive sheep had seropositive offspring. Within dams that had any abortion, seropositive sheep were more likely than seronegative ones to have female descendants that aborted (OR = 8.12). The slight increase in seropositivity with the age, the low percentage of animals with postnatal seroconversion or with low avidity antibodies, and the seropositivity of one flock dog, indicated that horizontal transmission might have some relevance in this flock. A control programme based on selective culling of seropositive sheep and replacement with seronegative animals was effective in reducing the abortion rate to 7.2%.

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Section: Discussionsupporting

confidence: 83%

Dynamics of Neospora caninum-Associated Abortions in a Dairy Sheep Flock and Results of a Test-and-Cull Control Programme

et al. 2021

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“…Finally, the isolation of the parasite—A key step in the study of this disease—has rarely been undertaken, with only three studies—one each in Japan, Brazil, and Spain—reporting its isolation [ 147 , 148 , 149 ]. Ovine N. caninum isolates are very valuable, as they enable further phenotypic characterizations via in vivo and in vitro experiments, allowing comparisons to be made with canine and bovine isolates.…”

Section: Impact Of Ovine Neosporosis In Reproductive Failurementioning

confidence: 99%

“…Koyama et al [ 147 ] were the first to isolate N. caninum specifically from the brain of a pregnant, clinically healthy sheep using a murine bioassay; similarly, Pena et al [ 148 ] achieved isolation of N. caninum from two 4-month-old sheep via bioassays in dogs. However, García-Sánchez et al [ 149 ] recently obtained two isolates of N. caninum from naturally infected lambs in a flock with chronic infection and endogenous (vertical) transmission across different generations of sheep. The parasite was isolated using a murine bioassay and the MARC-145 cell line, and molecular characterization revealed that the multilocus genotype (MLG) was unique, but related to Spanish, Argentinean, Mexican, Brazilian, and German isolates.…”

Section: Impact Of Ovine Neosporosis In Reproductive Failurementioning

confidence: 99%

Ovine Neosporosis: The Current Global Situation

Benavides

González-Warleta

Arteche-Villasol

et al. 2022

Animals

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In the past 20 years, Neospora caninum infection in sheep has been reported in at least 31 countries worldwide from all sheep-rearing continents (Europe, Asia, the Americas, Africa, and Oceania), and its role as an abortifacient agent is becoming more evident. Most studies of ovine neosporosis have focused on its epidemiology, based primarily on serological analysis, with only a few studies investigating the actual presence of the parasite by PCR and/or IHC. Individual seroprevalence rates were highly variable between countries, and even between regions within the same country, ranging from 0.0% to 67.4% positive. Furthermore, most of the studies were not directly comparable due to differences in experimental designs, sample sizes, husbandry systems, ecological factors, and serological tests (e.g., IFAT, ELISA, MAT, Western blot). The latter, along with the scarcity of studies on the relevance of N. caninum as an abortifacient agent, may bias the perception of the importance of this disease. This review summarizes the situation of N. caninum infection in sheep using all available published studies describing natural ovine neosporosis. The epidemiology shows that ovine neosporosis is found worldwide, and it poses a relevant risk to the sustainability of sheep flocks.

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Molecular detection and phylogenic characterization of Neospora caninum in naturally infected sheep in Alborz and Qazvin provinces, the north of the central region of Iran

Karimi,

Nasiri,

Jameie

et al. 2023

Parasitol Res

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Isolation and genetic characterization of Neospora caninum from naturally infected sheep

Cited by 6 publications

References 20 publications

Dynamics of Neospora caninum-Associated Abortions in a Dairy Sheep Flock and Results of a Test-and-Cull Control Programme

Dynamics of Neospora caninum-Associated Abortions in a Dairy Sheep Flock and Results of a Test-and-Cull Control Programme

Ovine Neosporosis: The Current Global Situation

Molecular detection and phylogenic characterization of Neospora caninum in naturally infected sheep in Alborz and Qazvin provinces, the north of the central region of Iran

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