Isolation and genomics of ten novel Shewanella species from mangrove wetland

Liu, Guo-Hong; Yang, Shang; Rao, Manik Prabhu Narsing; Han, Seunghee; Xie, Cheng-Jie; Alwathnani, Hend A.; Herzberg, Martin; Rensing, Christopher; Zhou, Shungui

doi:10.1099/ijsem.0.005929

Cited by 3 publications

(11 citation statements)

References 65 publications

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“…The presence of ubiquinone as the major quinone and menaquinone as the minor quinone aligns with the characteristic quinone pattern observed in various members of the genus Shewanella [2,5,[7][8][9]. Nonetheless, the specific proportions of these quinones vary among different Shewanella species [2,5]. On the other hand, the only respiratory quinone detected in strain KJ40-1 T was Q-8, consistent with previous reports on Vibrio species [16][17][18].…”

Section: Chemotaxonomysupporting

confidence: 90%

“…The respiratory quinones Q-8 (44.0 %), Q-7 (36.2 %), MK-7 (11.3 %), and MMK-7 (8.5 %) were detected in strain KJ10-1 T . The presence of ubiquinone as the major quinone and menaquinone as the minor quinone aligns with the characteristic quinone pattern observed in various members of the genus Shewanella [2,5,[7][8][9]. Nonetheless, the specific proportions of these quinones vary among different Shewanella species [2,5].…”

Section: Chemotaxonomysupporting

confidence: 77%

“…The genus Shewanella, which belongs to the family Shewanellaceae, order Alteromonadales, phylum Pseudomonadota, was initially proposed by MacDonell and Colwell, with Shewanella putrefaciens as the type species [1]. As of March 2024, the genus encompasses a total of 108 species, both validly and invalidly published (https://lpsn.dsmz.de/genus/Shewanella), originating from diverse environments, including mangrove wetlands, fish, freshwater, seawater, marine sediments, marine algae, activated sludge, and tube worms [2][3][4][5][6][7][8][9]. Members of the genus Shewanella are typically Gram-stain-negative, oxidase-and catalase-positive, and facultative aerobic.…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, these bacteria typically exhibit a non-pigmented to pale pink coloration. Additionally, they contain C 16 : 0 , C 17 : 1 ω8c, iso-C 15 : 0 , and summed feature 3 (comprising C 16 : 1 ω7c and/or C 16 : 1 ω6c) as major fatty acids and ubiquinone-7 (Q-7), ubiquinone-8 (Q-8), menaquinone-7 (MK-7), and methylmenaquinone-7 (MMK-7) as respiratory quinones [2][3][4][5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

“…Strains: 1, KJ10-1 T (this study); 2, S. saliphila JCM 32304 T[2]; 3, S. algicola JCM 31091 T[4]; 4, S. inventionis KCTC 42807 T…”

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confidence: 99%

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Shewanella phaeophyticola sp. nov. and Vibrio algarum sp. nov., isolated from marine brown algae

Butt,

Jung,

Kim

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

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Two Gram-stain-negative, rod-shaped bacteria, designated as strains KJ10-1T and KJ40-1T, were isolated from marine brown algae. Both strains were catalase-positive, oxidase-positive, and facultative aerobic. Strain KJ10-1T exhibited optimal growth at 25 °C, pH 7.0, and 3 % NaCl, whereas strain KJ40-1T showed optimal growth at 25 °C, pH 7.0, and 2 % NaCl. The respiratory quinones of strain KJ10-1T were ubiquinone-8, ubiquinone-7, menaquinone-7, and methylated menaquinone-7, while the respiratory quinone of strain KJ40-1T was only ubiquinone-8. As major fatty acids, strain KJ10-1T contained C16 : 0, C17 : 1 ω8c, iso-C15 : 0, and summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and strain KJ40-1T contained C16 : 0 and summed features 3 and 8 (C18 : 1 ω7c and/or C18 : 1 ω6c). The major polar lipids in strain KJ10-1T were phosphatidylethanolamine, phosphatidylglycerol, and an unidentified aminolipid, whereas those in strain KJ40-1T were phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. The DNA G+C contents of strains KJ10-1T and KJ40-1T were 42.1 and 40.8 mol%, respectively. Based on 16S rRNA gene sequences, strains KJ10-1T and KJ40-1T exhibited the closest relatedness to Shewanella saliphila MMS16-UL250T (98.6 %) and Vibrio rumoiensis S-1T (95.4 %), respectively. Phylogenetic analyses, based on both 16S rRNA and 92 housekeeping genes, showed that the strains formed distinct phylogenic lineages within the genera Shewanella and Vibrio. Digital DNA–DNA hybridization and orthologous average nucleotide identity values between strain KJ10-1T and other Shewanella species, as well as between strain KJ40-1T and other Vibrio species, were below the thresholds commonly accepted for prokaryotic species delineation. Based on the phenotypic, chemotaxonomic, and phylogenetic data, strains KJ10-1T and KJ40-1T represent novel species of the genera Shewanella and Vibrio, respectively, for which the names Shewanella phaeophyticola sp. nov. and Vibrio algarum sp. nov. are proposed, respectively. The type strains of S. phaeophyticola and V. algarum are KJ10-1T (=KACC 22589T=JCM 35409T) and KJ40-1T (=KACC 22588T=JCM 35410T), respectively.

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Section: Chemotaxonomysupporting

confidence: 90%

Section: Chemotaxonomysupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Strains: 1, KJ10-1 T (this study); 2, S. saliphila JCM 32304 T[2]; 3, S. algicola JCM 31091 T[4]; 4, S. inventionis KCTC 42807 T…”

mentioning

confidence: 99%

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Shewanella phaeophyticola sp. nov. and Vibrio algarum sp. nov., isolated from marine brown algae

Butt,

Jung,

Kim

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

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Shewanella youngdeokensis sp. nov. Isolated from Marine Sediment and Sea Water of East Sea in Republic of Korea

Lee,

Jeong,

Hwang

et al. 2024

Microbiology

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The strain DAU334T, which was obtained from samples of sea water and sediments collected from the East Sea in the Republic of Korea, is a rod-shaped, Gram-stain-negative bacterium. It is facultative and positive for the oxidase and catalase enzymes. Based on the analysis of 16S rRNA sequences, it was determined that strain DAU334T had the highest degree of similarity to several strains of Shewanella species, i.e., S. schlegeliana HRKA1T (99%), S. halifaxensis HAE-EB4T (99%), S. pneumatophori SCRC-2738T (99%), S. sairae SM2-1T (99%), and S. marinintestina IK-1T (1419/1440, 99%), respectively. The complete genome sequence consisted of one contig with 4 343 646 bp, and the genome G+C content was 43.12%. The average nucleotide identity (ANI), digital DNA-DNA hybridization (dDDH), and average amino acid identity (AAI) between strain DAU334T and its closest relatives were 77.85‒71.10, 82.14‒67.07, and 22.60‒19.70%, respectively. The major fatty acids (>10%) of strain DAU334T were iso-C13:0 and Summed feature 3 (C16:1 w7c and/or C16:1 w6c). The respiratory quinones were ubiquinone 7 (Q7), ubiquinone 8 (Q8), menaquinone 7 (MK-7), menaquinone 8 (MK-8), methylated menaquinone 7 (MMK-7), and an unidentified one. According to the results of polyphasic analyses, the strain DAU334T belongs to a novel species within the genus Shewanella, for which the name Shewanellayoungdeokensis sp. nov. (type strain DAU334T = KCTC 72428T = JCM 35745T = KCCM 43479T) is proposed.

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Polyphasic Characterization and Genomic Insights into an Aerobic Denitrifying Bacterium, Shewanella zhuhaiensis sp. nov., Isolated from a Tidal Flat Sediment

Liu,

Pei,

et al. 2023

Microorganisms

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A new, facultatively anaerobic, light-yellow, and rod-shaped bacterium designated as 3B26T isolated from Qi’ao Island’s tidal flat sediment was identified. Strain 3B26T can hydrolyze gelatin, aesculin, and skim milk. The major cellular fatty acids were identified as iso-C15:0, referred to as summed feature 3, and C16:0; the polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, and phospholipid; and the quinones contained Q-7, Q-8, MK-7, and MMK7. The genomic size of strain 3B26T was 4,682,650 bp, and its genomic DNA G + C content was 54.8%. While a 16S rRNA gene-based phylogenetic analysis confirmed that strain 3B26T belongs to the genus Shewanella, both phylogenomic inference and genomic comparison revealed that strain 3B26T is distinguishable from its relatives, and digital DNA-DNA hybridization (dDDH) values of 24.4–62.6% and average nucleotide identities (ANIs) of 83.5–95.6% between them were below the 70% dDDH and 96% ANI thresholds for bacterial species delineation. Genomic functional analysis demonstrated that strain 3B26T possesses complete gene clusters of eicosapentaenoic acid biosynthesis and denitrification. Based on the evidence above, strain 3B26T is considered to represent a novel species of the genus Shewanella, and the name Shewanella zhuhaiensis sp. nov. (type strain 3B26T = GDMCC 1.2057T = KCTC 82339T) is proposed.

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Isolation and genomics of ten novel Shewanella species from mangrove wetland

Cited by 3 publications

References 65 publications

Shewanella phaeophyticola sp. nov. and Vibrio algarum sp. nov., isolated from marine brown algae

Shewanella phaeophyticola sp. nov. and Vibrio algarum sp. nov., isolated from marine brown algae

Shewanella youngdeokensis sp. nov. Isolated from Marine Sediment and Sea Water of East Sea in Republic of Korea

Polyphasic Characterization and Genomic Insights into an Aerobic Denitrifying Bacterium, Shewanella zhuhaiensis sp. nov., Isolated from a Tidal Flat Sediment

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