2014
DOI: 10.1111/jeu.12197
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Isolation and Genotyping of Acanthamoeba Strains from Soil Sources from Jamaica, West Indies

Abstract: Acanthamoeba spp. are opportunistic pathogens that are ubiquitous in nature. Many species of this genus are responsible for a fatal encephalitis and keratitis in humans and other animals. Seventy-two soil samples were collected from the parishes across Jamaica and assessed for the presence of Acanthamoeba spp. Cultivation was carried out on non-nutrient agar plates seeded with heat killed Escherichia coli. PCR and sequencing of the DF3 region were carried out in order to genotype the isolated strains of Acanth… Show more

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Cited by 28 publications
(18 citation statements)
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“…Acanthamoeba spp. are the most common free-living protozoa, isolated from public water supplies, swimming pools, bottled water, distilled water bottles, ventilation ducts, the water-air interface, air-conditioning units, soil, beaches, vegetables, air, surgical instruments, and contact lenses, indicating the ubiquitous nature of these organisms (Cruz and Rivera, 2014;Todd et al, 2015;Al-Herrawy et al, 2015). Acanthamoeba species can cause various diseases such as keratitis and granulomatous amoebic encephalitis, which is often fatal in immune-compromised as well as immunocompetent patients (Król-Turmińska and Olender, 2017).…”
Section: Introductionmentioning
confidence: 99%
“…Acanthamoeba spp. are the most common free-living protozoa, isolated from public water supplies, swimming pools, bottled water, distilled water bottles, ventilation ducts, the water-air interface, air-conditioning units, soil, beaches, vegetables, air, surgical instruments, and contact lenses, indicating the ubiquitous nature of these organisms (Cruz and Rivera, 2014;Todd et al, 2015;Al-Herrawy et al, 2015). Acanthamoeba species can cause various diseases such as keratitis and granulomatous amoebic encephalitis, which is often fatal in immune-compromised as well as immunocompetent patients (Król-Turmińska and Olender, 2017).…”
Section: Introductionmentioning
confidence: 99%
“…The strains were isolated from the soil collected near electroplating plants from Shandong, Jiangxi, Jilin, Liaoning, and Heilongjiang provinces. Briefly, 1 g of soil of each sample was dissolved in 10 mL sterile water, and then 100 µL of this soil suspension was added to LB medium with 2 mM Cr(VI) [15,35]. After incubating for 72 h at 37 • C, with agitation at 180 rpm, the enriched culture solution was then spread-plated on LB-solid medium with 2 mM Cr(VI) [35].…”
Section: Isolation Of the Strainsmentioning
confidence: 99%
“…Briefly, 1 g of soil of each sample was dissolved in 10 mL sterile water, and then 100 µL of this soil suspension was added to LB medium with 2 mM Cr(VI) [15,35]. After incubating for 72 h at 37 • C, with agitation at 180 rpm, the enriched culture solution was then spread-plated on LB-solid medium with 2 mM Cr(VI) [35]. The selected clones were further purified using the streak plating method [36].…”
Section: Isolation Of the Strainsmentioning
confidence: 99%
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