Isolation and genotyping of potentially pathogenic Vibrio alginolyticus associated with Ruditapes decussatus larva and juvenile mass mortalities

Mechri, Badreddine; Salem, Imen Ben; Medhioub, Amel; Medhioub, Mohamed Néjib; Aouni, Mahjoub

doi:10.1007/s10499-014-9862-7

Cited by 10 publications

(12 citation statements)

References 43 publications

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“…In fact, there is a well growth up to 8% of NaCl and 40% of strains were described with ability to grow also at 10% NaCl. On the contrary, V. parahamolyticus exhibits poor growth in media with 6% NaCl and 10% NaCl is inhibitory (Mechri et al 2015). Moreover, both are able to growth between 15 and 42°C.…”

Section: Vibrio Alginolyticus and Vibrio Parahaemolyticusmentioning

confidence: 97%

“…In fact, several bacterial included in the Splendidus clade are considered pathogenic for bivalve molluscs (oyster, clam, scallop, mussel) (Revised by Beaz-Hidalgo et al 2009; Revised by Kwan and Bolch 2015;Vanhove et al 2015;Cheikh et al 2016) and also cephalopod molluscs (Farto et al 2003;Iehata et al 2016). Similarly, V. alginolyticus was pathogenic for carpet shell clam larval and juvenile (Gómez-León et al 2005;Mechri et al 2015), fish (Liu et al 2004;Zheng et al 2017), crustacean (Jayaprakash et al 2006;Xu et al 2013), scallop (Riquelme et al 1996), red abalone (Anguiano-Beltrán et al 1998), associated with infected Chilean octopus eggs (Iehata et al 2016), with ability to cause skin lesions to young octopus (Hanlon and Forsythe 1990), and isolated from skin ulcers and/or different organs of cultured cuttlefish (Sangster and Smolowitz 2003). V. proteolyticus has been previously identified as part of the Vibrio consortium isolated from diseased corals (Cervino et al 2008), and were identified as pathogens of Artemia spp.…”

Section: Pathogenicity Of Culturable Bacteriamentioning

confidence: 99%

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Bacteria-Affecting Cephalopods

Farto

Fichi

Gestal

et al. 2019

Handbook of Pathogens and Diseases in Cephalopods

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Bacterial pathogens contribute to obtain an unsuccessful production of cephalopods. An updated overview of the knowledge of these pathogens must be a valuable tool to improve their aquarium maintenance and aquaculture. The present work provides a description of the main bacterial pathogens associated with larval stages of cultured Octopus vulgaris, and juvenile and adults of several cephalopods. Vibrio species, reported with ability to cause vibriosis in aquaculture, are the main bacteria associated with skin lesions in adults. Different species of Pseudomonas and Aeromonas, among others, have also been detected. Furthermore, gram-positive bacteria such as Bacillus have been also described. Among them, V. alginolyticus, V. carchariae, V. parahaemolyticus, V. splendidus and V. lentus have also been isolated from sterile organs or fluids of animals and their potential as invaders proved. However, only V. alginolyticus or V. lentus has the ability to cause lesions, and, in addition, the last one is proved as the causative agent of death in octopuses. Other organs such as eyes of squids are also colonized by Vibrio species or Micrococcus sp., and recently Photobacterium swingsii and Lactococcus garvieae have been reported associated with a retrobulbar lesion in octopus. Rickettsial-like organisms (RLO) are also detected in the gills of the octopus, having a detrimental effect on the respiratory gaseous exchange of the animals. Cultures of octopus paralarvae show a genetically diverse community comparable to those reported previously from other marine hatcheries. Bacteria included in the Splendidus clade is the dominant group in all conditions, except in one of them, where V. alginolyticus, V.

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Section: Vibrio Alginolyticus and Vibrio Parahaemolyticusmentioning

confidence: 97%

Section: Pathogenicity Of Culturable Bacteriamentioning

confidence: 99%

Bacteria-Affecting Cephalopods

Farto

Fichi

Gestal

et al. 2019

Handbook of Pathogens and Diseases in Cephalopods

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“…In the literature, it is reported that antibiotic treatments during larval rearing prevent bacterial contamination, which increases successful larval rearing (Robert et al 1996;Uriarte et al 2001;Torkildsen et al 2005). However, the overuse of antibiotics in treatment of mollusk aquaculture may contribute to an increase in the level of antimicrobial resistance (Mechri et al 2013(Mechri et al , 2015. The introduction of a flowthrough system instead of closed-circuit water system in larval rearing tanks eliminated the use of antibiotics by improving water quality (Southgate and Ito 1998;Magnesen et al 2006).…”

Section: Discussionmentioning

confidence: 99%

“…Thus, FF is often used to control bacterial disease outbreaks in mollusk larval hatcheries (Torkildsen et al 2002;Torkildsen and Magnesen 2004;Miranda et al 2013). However, several reports have demonstrated that the extensive use of antibiotics in aquaculture could facilitate the emergence and the selection of resistant bacteria (Laganà et al 2011;Mechri et al 2015). Previous studies of antimicrobial resistance in fish pathogenic bacteria have reported the occurrence of the floR gene as the major facilitator superfamily and code for efflux proteins that export florfenicol out of the cell (Miranda et al 2013;Schwarz et al 2004).…”

Section: Discussionmentioning

confidence: 99%

“…In fact, larval culture tanks water is completely exchanged at least three times per week, allowing microbes to increase their biomass in the rearing water (Andersen et al 2000;Torkildsen and Magnesen 2004). Despite the use of antibacterial agents as prophylactic therapy, we have been confronted with recurrent epizootic disease outbreaks in larval and early post-larval stages, causing high mortality (Mechri et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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Impacts of rearing techniques on growth, survival and bacterial microbiota of carpet shell clam (Ruditapes decussatus) larvae

et al. 2016

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In this study, we investigate the impacts of two water treatment regimes, the closed aquaculture system (CAS) and flow-through system (FTS), on the carpet shell clam (Ruditapes decussatus) larval microbiota, growth and survival. A bacteriological barrier (B) was created among breeder stage treated by florfenicol (FF, 8 mg L -1 day -1 per 5 days). After spawning, larvae were maintained in five different rearing techniques: CAS; FTS; B ? CAS; B ? FTS and B ? CAS ? FF (8 mg L -1 day -1 per 3 days). Significant decrease was observed in both total bacterial count and presumptive Vibrio count (PVC) in FF-treated breeders. Besides, larvae reared in B ? FTS showed significantly lower PVC than larvae maintained in other rearing techniques. It was also determined that growth and survival of larvae reared in B ? CAS ? FF and B ? FTS were significantly better than those reared in other systems. One hundred and twelve Vibrio strains, isolated from breeders, eggs and larvae, were subjected to some bacteriological tests (API 20E strips, vibriostatic agent O/129, growth at different temperatures and salinity). Vibrio alginolyticus was the predominant isolated species (36.61 %), followed by V. fluvialis (25 %), V. vunificus (17.86 %), V. cholera (13.39 %) and V. paraheamolyticus (7.14 %). These results may contribute to the understanding of the effect of rearing techniques on the bacterial load, survival and growth of early life stages of R. decussatus larvae.

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