Isolation and Histopathological Changes Associated with Non-Tuberculous Mycobacteria in Lymph Nodes Condemned at a Bovine Slaughterhouse

Hernández-Jarguín, Angélica; Martı́nez-Burnes, Julio; Molina-Salinas, Gloria María; Cruz-Hernández, Ned I. de la; Palomares-Rangel, José L.; Mayagoitia, Alfonso López; Barrios-García, Hugo

doi:10.3390/vetsci7040172

Cited by 8 publications

(5 citation statements)

References 54 publications

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“…But most of the species detected in this work could be pathogenic if conditions for infection development are favourable (e.g., immunocompromised animals, high-dose exposure etc.). In fact, in addition to a few strict pathogens (Maa and Map) (Turenne et al, 2007(Turenne et al, , 2008 many of these NTM have already been described as opportunistic pathogens of animals and humans (Pereira et al, 2020) & Vincent, 2001;Biet & Boschiroli, 2014;Gcebe & Hlokwe, 2017;Ghielmetti et al, 2018;Ghielmetti et al, 2021;Hernández-Jarguín et al, 2020;Kik et al, 2010;Katale et al, 2014;Timm et al, 2019;Zeng et al, 2013). With regard to the isolates belonging to unclassified mycobacteria strains, only one identified as IEC1808 was cultured from lesioned tissues belonging to a wild boar from CR.…”

Section: Discussionmentioning

confidence: 99%

“…The remaining NTM species of this study were apparently not related with the presence of lesions in their hosts. But other authors have previously pointed out to some of those NTM as the cause of lesions or clinical signs in the same or other hosts ( M. alvei , M. confluentis , M. engbaekii , M. fortuitum , M. florentinum , M. interjectum , M. intermedium , M. kansasii , M. kumamotonense , M. nebraskense , M. scrofulaceum , M. senuense and M. septicum ) (Bercovier & Vincent, 2001; Biet & Boschiroli, 2014; Gcebe & Hlokwe, 2017; Ghielmetti et al., 2018; Ghielmetti et al., 2021; Hernández‐Jarguín et al., 2020; Kik et al., 2010; Katale et al., 2014; Timm et al., 2019; Zeng et al., 2013). With regard to the isolates belonging to unclassified mycobacteria strains, only one identified as IEC1808 was cultured from lesioned tissues belonging to a wild boar from CR.…”

Section: Discussionmentioning

confidence: 99%

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Beyond tuberculosis: Diversity and implications of non‐tuberculous mycobacteria at the wildlife–livestock interface

Varela-Castro

Barral

Arnal

et al. 2022

Transbounding Emerging Dis

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Non‐tuberculous mycobacteria (NTM) circulate between the environment, animals and humans entailing a double concern: their ability to interfere with tuberculosis diagnosis and their potential to cause infections in their hosts. However, published records on NTM infections in animals are still scarce. The aims of the present study were to describe the diversity of NTM circulating among wild and domestic species from Spain and to analyze their implications as potential pathogenic microorganisms or as sources of interferences in the diagnosis of bovine tuberculosis. Overall, 293 NTM isolates of 277 animals were obtained from tissue samples collected between 2012 and 2019, and analyzed through a multigene approach for mycobacteria identification. Thirty‐one species were identified, being Mycobacterium avium subsp. avium (Maa) and M. avium subsp. hominissuis (Mah), but also M. bouchedurhonense, M. nonchromogenicum and M. lentiflavum, the most abundant ones. Maa and M. lentiflavum were isolated in several animals showing tuberculosis‐like lesions. Maa, Mah and M. nonchromogenicum were recovered from many cattle that had reacted to the tuberculin skin test. Other NTM were also associated to these phenomena. These four mycobacterial species were geographically associated between wild boar and other hosts. The findings of the present study suggest that a high diversity of NTM circulates among wildlife and livestock. Wild boar and M. avium seem to play a relevant role in this epidemiological scenario.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Beyond tuberculosis: Diversity and implications of non‐tuberculous mycobacteria at the wildlife–livestock interface

Varela-Castro

Barral

Arnal

et al. 2022

Transbounding Emerging Dis

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“…Additionally, the infection of cattle with atypical Mycobacterium may make the diagnosis of tuberculosis difficult. It has been proven that mycobacteria belonging to the MAC are the cause of false-positive results of the tuberculin skin tests, resulting in the wrong decision to slaughter positively reacting individuals [18,19].…”

Section: Discussionmentioning

confidence: 99%

Pulmonary mycobacteriosis of sitatunga antelope caused by <i>M. avium</i> ssp. <i>hominissuis</i>

Radulski¹,

Kalicki²,

Krajewska‐Wędzina³

et al. 2022

Ann Agric Environ Med.

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Introduction and Objective.Mycobacteriosis are diseases caused by acid-fast mycobacteria other than M. leprae and tuberculous mycobacteria. Animal mycobacteriosis is often caused by M. avium ssp. hominissuis. Many species of animals are susceptible to infection with this bacterium, even those kept in Zoological Gardens. The aim of the study was to determine the species of bacterium responsible for causing the disease in the tested animals. Materials and method. Tissue samples of two male sitatunga antelopes (Tragelaphus spekii) were analyzed. Lymph node and lung samples were subjected to anatomical examination and Ziehl-Neelsen staining. Real-time PCR was performed to confirm or rule out tuberculosis mycobacteria infection. In order to isolate the bacterial strain, tissue samples were inoculated on both solid and liquid media. HainLifescience CM tests, mass spectrometry and New Generation Sequencing were used to determine the mycobacterial species. Results. Results showed that atypical mycobacteria are responsible for the antelope disease. The results of the HainLifescience CM test and mass spectrometry indicated that the mycobacterium responsible for causing mycobacteriosis was M. avium. New Generation Sequencing helped to identified a subspecies that was M. avium ssp. hominissuis. Conclusions. The sitatunga antelope is an animal susceptible to infection by M. avium ssp. hominissuis. Considering the wide range of hosts and the easiness of interspecies transmission of the pathogen, as well as its zoonotic nature, the mycobacteriosis induced by this microorganism should not be underestimated.

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“…Cross-reactivity in bTB tests has been described in animals exposed to NTMs; M. fortui-tum and M. kansasii may sensitise cattle and lead to false positive results in immunological assays, as well as cause disease in a variety of hosts 7,34,38,45 . Other NTMs, such as M. flavescens and M. szulgai, contain the RD1 genetic region, which may cause cross-reactivity in bTB diagnostic tests, as well as cause granulomatous disease 18,46,47 .…”

Section: Discussionmentioning

confidence: 99%

Identification and Characterisation of Nontuberculous Mycobacteria that may Impede the Diagnosis of Bovine Tuberculosis in African Buffaloes (Syncerus caffer), South Africa

Clarke¹,

Kerr²,

Warren³

et al. 2022

Preprint

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Diagnosis of bovine tuberculosis (bTB) may be confounded by immunological cross-reactivity to Mycobacterium bovis antigens when animals are sensitised by certain nontuberculous mycobacteria (NTMs). Therefore, this study aimed to investigate NTM species diversity in African buffalo (Syncerus caffer) respiratory secretions and tissue samples, using a combination of novel molecular tools. Oronasal swabs were collected opportunistically from 120 immobilised buffaloes in historically bTB-free herds. In addition, bronchoalveolar lavage fluid (BALF; n=10) and tissue samples (n=19) were obtained during post-mortem examination. Mycobacterial species were identified directly from oronasal swab samples using the Xpert MTB/RIF Ultra qPCR (14/120 positive) and GenoType CMdirect (104/120 positive). In addition, all samples underwent mycobacterial culture, and PCRs targeting hsp65 and rpoB were performed. Overall, 55 NTM species were identified in 36 mycobacterial culture-positive swab samples with presence of esat-6 or cfp-10 detected in 20 of 36 isolates. The predominant species were M. avium complex and M. komanii. Nontuberculous mycobacteria were also isolated from 6 of 10 culture-positive BALF and 4 of 19 culture-positive tissue samples. Our findings demonstrate that there is a high diversity of NTMs present in buffaloes, and further investigation should determine their role in confounding bTB diagnosis in this species.

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Isolation and Histopathological Changes Associated with Non-Tuberculous Mycobacteria in Lymph Nodes Condemned at a Bovine Slaughterhouse

Cited by 8 publications

References 54 publications

Beyond tuberculosis: Diversity and implications of non‐tuberculous mycobacteria at the wildlife–livestock interface

Beyond tuberculosis: Diversity and implications of non‐tuberculous mycobacteria at the wildlife–livestock interface

Pulmonary mycobacteriosis of sitatunga antelope caused by <i>M. avium</i> ssp. <i>hominissuis</i>

Identification and Characterisation of Nontuberculous Mycobacteria that may Impede the Diagnosis of Bovine Tuberculosis in African Buffaloes (Syncerus caffer), South Africa

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