Isolation and identification of a cellulolytic bacterium from the Tibetan pig's intestine and investigation of its cellulase production

Yang, Wei; Meng, Fan-Xu; Peng, Jian; Han, Peng; Fang, Fang; Ma, Li; Cao, Binyun

doi:10.1016/j.ejbt.2014.08.002

Cited by 77 publications

(61 citation statements)

References 26 publications

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“…The Bacillus sp. isolated by Yang et al [41] and Bairagi et al [42] showed similar results of organic nitrogen source for cellulase production. However, the B. subtilis could utilize both the inorganic and organic nitrogen sources for cellulase production [43].…”

Section: Effect Of Different Nitrogen Sources In Culture Medium Durinsupporting

confidence: 88%

Characterization of Novel Cellulase-producing Bacteria Isolated From Rotting Wood Samples

Paudel

Qin

2015

Appl Biochem Biotechnol

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Seventeen bacterial isolates were screened for their cellulase activity by carboxymethyl cellulose (CMC) plate assay. The bacterial strain K1 showed the largest depolymerized region in CMC plate assay and was further studied for quantitative cellulase activity. On the basis of 16S rDNA sequence analysis, the strain K1 was found to be Bacillus sp. This strain produced the maximum CMCase at pH 6 and 50 °C in the presence of peptone (1%) as a source of nitrogen. The CMCase activity was stimulated by Ca(2+) (2 mM) by 20% over the control. The CMCase activity of this Bacillus sp. K1 was highly induced when lactose was used as a source of carbon during fermentation.

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Section: Effect Of Different Nitrogen Sources In Culture Medium Durinsupporting

confidence: 88%

Characterization of Novel Cellulase-producing Bacteria Isolated From Rotting Wood Samples

Paudel

Qin

2015

Appl Biochem Biotechnol

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“…The result of this study are similar to those of other researchers, in that the exponential phase of BY-2 Bacillus subtilis took 0-20 h, the stationary phase took 20-39 h, and fermentation for the production of cellulase enzymes took 24 h [21]. The production of cellulase enzymes was conducted for 24 h [22].…”

Section: Growth Curve Of Selected Bacterial Isolatessupporting

confidence: 79%

Isolation, Cellulase Activity Test and Molecular Identification of Selected Cellulolytic Bacteria Indigenous Rice Bran

et al. 2018

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“…This phase is formed because the bacteria undergo rapid cell Sadhu et al (2014), cellulase production of Bacillus sp best at age 8 hours (incubation period of 2-10 days). But, acccording to Yang et al (2014) examination of its growth characteristics Bacillus subtilis BY-2 showed that its growth curve entered the logarithmic phase after 8-12 h and the stable growth phase being between 20 and 40 h. This difference is caused by different types of bacteria used, as well as environmental conditions. In this phase of cellulase enzyme formed and are classified as primary metabolites (Madigan et al, 2009;Sadhu et al, 2014).…”

Section: The Growth Of Isolate Bacteria Ke-b6mentioning

confidence: 99%

Screening Cellulolytic Bacteria from the Digestive Tract Snail (Achatina fulica) and Test the Ability of Cellulase Activity

2016

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On the research of enzyme production levels observed cellulase produced by bacteria in the digestive tract of the isolation of the Snail (Achatina fulica). Isolation of bacteria based on the ability of bacteria to grow on CMC media. The purpose of this study was to determine cellulase activity by cellulolytic bacteria. Some bacterial isolates were identified as cellulolytic bacteria, they were KE-B1, KE-B2, KE-B3, KE-B4, KE-B5, and KE-B6. Isolates KE-B6 was the best isolates. Furthermore KE-B6 isolates were grown on media production to determine the pattern of growth and enzyme activity. Measurement of cell growth was conducted by inoculating starter aged 22 hours at CMC production of liquid medium. Cellulase enzyme activity measurements was performed by the DNS method. The results showed that the highest activity by new isolate bacteria KE-B6 and its value of the activity of 0.4539 U/mL, growth rate (μ) 0.377/hour and generation time (g) 1.84 hour. This research expected cellulase of producing bacteria were easy, inexpensive and efficient. This enzyme can be used as an enzyme biolytic once expected to replace expensive commercial enzyme. The biotylic enzyme can be applied to strains improvement (protoplast fusion).

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Isolation and identification of a cellulolytic bacterium from the Tibetan pig's intestine and investigation of its cellulase production

Cited by 77 publications

References 26 publications

Characterization of Novel Cellulase-producing Bacteria Isolated From Rotting Wood Samples

Characterization of Novel Cellulase-producing Bacteria Isolated From Rotting Wood Samples

Isolation, Cellulase Activity Test and Molecular Identification of Selected Cellulolytic Bacteria Indigenous Rice Bran

Screening Cellulolytic Bacteria from the Digestive Tract Snail (Achatina fulica) and Test the Ability of Cellulase Activity

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