Isolation and identification of a novel peptide from zein with antioxidant and antihypertensive activities

Abstract

The aim of this study is to isolate and identify a novel corn peptide (CP) from zein with antioxidant and antihypertensive activities based on bioactive-guided isolation procedures. Zein was hydrolyzed by using double enzymes immobilized with calcium alginate-chitosan beads and then fractionated and purified. The antioxidant and antihypertensive activities of the CP fractions were screened by in vitro and in vivo assays. The in vivo animal studies using spontaneously hypertensive rats (SHRs) confirmed the antihypertensive effects of the CP, and its angiotensin I-converting enzyme (ACE) inhibitory activity was retained after thermal treatment and simulated gastrointestinal digestion. The primary structure of CP-2-1 was identified by RP-HPLC-MS/MS and the amino acid sequence was determined as M-I/L-P-P with the molecular weight of 452.3 Da. CP-2-1 showed effective antioxidant and ACE inhibitory activities (IC50 values of 220 μg mL(-1) and 70.32 μg mL(-1), respectively) and it might be a potential candidate for antioxidant functional food or pharmaceuticals for hypertension.

Bioactive peptides as natural antioxidants in food products – A review

“…Memarpoor-Yazdi, Asoodeh, & Chamani 2012 A combination of two immobilized enzymes out of alcalase, pepsin, and trypsin were explored to hydrolyse zein protein (a by-product from corn oil production) (Wang et al, 2015).…”

Mass spectrometric characterization of the zein protein composition in maize flour extracts upon protein separation by SDS‐PAGE and 2D gel electrophoresis

“…Zein proteins have been applied for coating of tablets and in zein‐coated chitosan/tripolyphosphate nanoparticles for oral delivery of selenite . Zein‐coating has also been shown to protect peptides with antioxidant and antihypertensive activities .…”

Antioxidant, antihypertensive, and anticancer activities of the flavonoid fractions from green, oolong, and black tea infusion waste

“…11003800004211; Huafukang Bioscience Co., Beijing, China) and divided into five groups (model group, SHRs were intragastrically administered with the same volume of 0.9% normal saline (NS); positive control group, SHRs were intragastrically administered with captopril (10 mg/kg); experimental groups, SHRs were intragastrically administered with GTF, OTF and BTF at 50 mg/kg). After administration, the lowering effects of GTF, OTF and BTF on systolic (SBP) and diastolic blood pressure (DBP) were measured during 0–24 hr according to of Wang et al, () method and a noninvasive blood pressure meter was used (BP 2010, Beijing, China).…”

“…The ACE‐inhibitory effects were measured according to the method of Wang et al (). Firstly, 30 μL GTF, OTF or BTF, 80 μL, 5 mM hippuryl‐histidyl‐leucine (HHL) and 30 μL boric acid buffer (0.1 mol/L , pH 8.3) were mixtured and incubated for 5 min at 37°C.…”

“…After administration, the lowering effects of GTF, OTF and BTF on systolic (SBP) and diastolic blood pressure (DBP) were measured during 0-24 hr according to of Wang et al, (2015) method and a noninvasive blood pressure meter was used (BP 2010, Beijing, China).…”