Isolation and Identification of a Bacterial Agent Causing Soft Rot in Chinese Cabbage

Lee, Young‐Eun; Jeong, Jin-Ju; Volynchikova, Elena; Kim, Ki Deok

doi:10.33147/lsnrr.2019.27.1.41

Cited by 2 publications

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“…odoriferum ( Pco ), and P. polaris , they can also cause bacterial soft rot. Moreover, other bacteria such as Pseudomonas sp., Xanthomonas spp., and Pantoea agglomerans have also been reported as causal agents of bacterial soft rot ( Guo et al, 2019 ; Lee et al, 2019 ; Chen et al, 2021 ). To address the significant losses caused by bacterial soft rot, extensive research has been conducted.…”

Section: Discussionmentioning

confidence: 99%

“…To isolate pathogenic bacteria from Chinese cabbage stems exhibiting soft rot symptoms, the method described by Lee et al (2019) was used. Specifically, the junction areas of the stems (the area between infected or not by the pathogen) were cut into 30 × 30 mm pieces.…”

Section: Methodsmentioning

confidence: 99%

“…pekinensis , has originated in China and eastern Asia since the fifth century ( Kalloo and Rana, 1993 ). It is one of the largest cultivated and consumed vegetables in China these days and is becoming more and more popular worldwide ( Li J. et al, 2022 ) due to its richness in various minerals, vitamins, carotenoids, flavonols, and glucosinolate ( Podsedek, 2007 ; Lai et al, 2010 ; Lee et al, 2019 ). However, bacterial soft rot disease, one of the most prevalent diseases affecting Chinese cabbage, poses a significant challenge to its production and supply ( Alvarado et al, 2011 ; Liu et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

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Response of Chinese cabbage (Brassica rapa subsp. pekinensis) to bacterial soft rot infection by change of soil microbial community in root zone

Li,

Ren,

Ibrahim

et al. 2024

Front. Microbiol.

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Chinese cabbage, scientifically known as Brassica rapa subsp. pekinensis, is a highly popular vegetable in China for its delectable taste. However, the occurrence of bacterial soft rot disease poses a significant threat to its growth and overall development. Consequently, this study aimed to explore the defense mechanisms employed by Chinese cabbage against bacterial soft rot disease. Specifically, the investigation focused on understanding the relationship between the disease and the microbial communities present in the soil surrounding the roots of Chinese cabbage. Significant disparities were observed in the composition of microbial communities present in the root-zone soil of healthy Chinese cabbage plants compared to those affected by Pectobacterium brasiliense-caused soft rot disease. The analysis of 16S rRNA gene high-throughput sequencing results revealed a lower abundance of Proteobacteria (8.39%), Acidobacteriot (0.85), Sphingomonas (3.51%), and Vicinamibacteraceae (1.48%), whereas Firmicutes (113.76%), Bacteroidota (8.71%), Chloroflexi (4.89%), Actinobacteriota (1.71%), A4b (15.52%), Vicinamibacterales (1.62%), and Gemmatimonadaceae (1.35%) were more prevalent in healthy plant soils. Similarly, the analysis of ITS gene high-throughput sequencing results indicated a reduced occurrence of Chytridiomycota (23.58%), Basidiomycota (21.80%), Plectosphaerella (86.22%), and Agaricomycetes (22.57%) in healthy soils. In comparison, Mortierellomycota (50.72%), Ascomycota (31.22%), Podospora (485.08%), and Mortierella (51.59%) were more abundant in healthy plant soils. In addition, a total of 15 bacterial strains were isolated from the root-zone soil of diseased Chinese cabbage plants. These isolated strains demonstrated the ability to fix nitrogen (with the exception of ZT20, ZT26, ZT41, ZT45, and ZT61), produce siderophores and indole acetic acid (IAA), and solubilize phosphate. Notably, ZT14 (Citrobacter freundii), ZT33 (Enterobacter cloacae), ZT41 (Myroides odoratimimus), ZT52 (Bacillus paramycoides), ZT58 (Klebsiella pasteurii), ZT45 (Klebsiella aerogenes), and ZT32 (Pseudomonas putida) exhibited significant growth-promoting effects as determined by the plant growth promotion (PGP) tests. Consequently, this investigation not only confirmed the presence of the soft rot pathogen in Chinese cabbage plants in Hangzhou, China, but also advanced our understanding of the defense mechanisms employed by Chinese cabbage to combat soft rot-induced stress. Additionally, it identified promising plant-growth-promoting microbes (PGPMs) that could be utilized in the future to enhance the Chinese cabbage industry.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Response of Chinese cabbage (Brassica rapa subsp. pekinensis) to bacterial soft rot infection by change of soil microbial community in root zone

Li,

Ren,

Ibrahim

et al. 2024

Front. Microbiol.

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Yozgat İli Beyaz Baş Lahana Üretim Alanlarında Bakteriyel Yumuşak Çürüklük Hastalığına Neden Olan Pectobacterium İzolatlarının Tanılanması

Öztürk¹,

Soylu²

2022

Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi

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Yozgat ili lahana üretim alanlarında 2018-2019 vejetasyon döneminde yumuşak çürüklük hastalık etmenlerinin tanılanmasına yönelik survey çalışmaları yapılmıştır. Yaprak, gövde ve kök kısımlarında sulu-ıslak lezyonlar ve çürüme belirtisi sergileyen dokulardan bakteriyel etmeninin izolasyonu için Crystal Violet Pectate (CVP) besi yeri kullanılmıştır. Farklı tarlalardan toplanan 24 şüpheli bitki örneğinden CVP besi yerinde çukur oluşturan 16 adet pektolitik izolat elde edilmiştir. Şeffaf, konveks ve kenarları krater formda olduğu tespit edilen bakteriyel izolatların gram negatif, fakültative anaerob, oksidaz negatif, katalaz pozitif, UV-ışık altında King B besi yerindeki kolonilerinin floresans parlama göstermediği belirlenmiştir. Patates dilimlerinde ve lahana bitkilerinde yumuşak çürüklük belirtilerine neden olan izolatların 37 ºC’de gelişebildiği, %5’lik NaCl içeren sıvı besi yerinde türbidite oluşturduğu ve tütün yapraklarında aşırı duyarlılık reaksiyonuna neden olduğu belirlenmiştir. Elde edilen 16 izolat ile yapılan PCR analizlerde, izolatların tamamı Pectobacterium spp. için spesifik Y1/Y2 primerleri ile 434 bç, P. carotovorum subsp. carotovorum için spesifik EXPCCF/EXCPCCR primerleri ile ise 550 bç büyüklüğünde PCR ürünü oluşturmuştur. Yapılan biyokimyasal, fizyolojik, patojenite ve moleküler analizler sonucu test edilen 16 izolatın P. carotovorum subsp. carotovorum olduğunu göstermiştir. Yapay inokulasyon yapılan lahana bitkilerinde patojenik izolatların sulu-ıslak lezyonlar şeklinde yumuşak çürüklük belirtilerine neden olduğu gözlemlenmiştir. P. carotovorum subsp. carotovorum’un Yozgat ili beyaz baş lahana üretim alanlarında yumuşak çürüklük hastalığının enfeksiyon kaynağı olduğu ilk kez bu çalışma ile tespit edilmiştir.

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Isolation and Identification of a Bacterial Agent Causing Soft Rot in Chinese Cabbage

Cited by 2 publications

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Response of Chinese cabbage (Brassica rapa subsp. pekinensis) to bacterial soft rot infection by change of soil microbial community in root zone

Response of Chinese cabbage (Brassica rapa subsp. pekinensis) to bacterial soft rot infection by change of soil microbial community in root zone

Yozgat İli Beyaz Baş Lahana Üretim Alanlarında Bakteriyel Yumuşak Çürüklük Hastalığına Neden Olan Pectobacterium İzolatlarının Tanılanması

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