Isolation and identification of a Cytobacillus oceanisediminis strain with ochratoxin A detoxification ability

Yang, Yan; Zhong, Weitong; Liu, Zhonghao; Xue, Xianli; Gao, Qiang; Wang, Depei; Zhang, Ying; Zhang, Jian

doi:10.1016/j.foodcont.2023.109797

Cited by 8 publications

(5 citation statements)

References 49 publications

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“…As shown in Figures 5A and 6A, the degradation rate of mycotoxins by the crude enzymes of B. subtilis ZJ-2019-1 increased with temperature rising from 30 • C to 70 • C. The maximum degradation rate of AFB1 and ZEN was 100% and 94.29%, respectively, at a temperature of 70 • C. The degradation rate of AFB1 and ZEN by the crude enzymes increased as the pH values ranged from 4.0 to 9.0, with the highest degradation rates of 73.91% and 46.11%, respectively. Some proteases may require EDTA as a co-factor for the degradation of mycotoxins [41]. The degradation rate of AFB1 by the crude enzymes of B. subtilis ZJ-2019-1 markedly decreased from 54.94% to 6.35% with an increase in EDTA concentration from 0 to 50 mM (Figure 5C).…”

Section: Effects Of Different Factors On Afb1 and Zen Degradation By ...mentioning

confidence: 98%

“…The degradation rate of AFB1 and ZEN by the crude enzymes increased as the pH values ranged from 4.0 to 9.0, with the highest degradation rates of 73.91% and 46.11%, respectively. Some proteases may require EDTA as a co-factor for the degradation of mycotoxins [41]. The degradation rate of AFB1 by the crude enzymes of B. subtilis ZJ-2019-1 markedly decreased from 54.94% to 6.35% with an increase in EDTA concentration from 0 to 50 mM (Figure 5C).…”

Section: Effects Of Different Factors On Afb1 and Zen Degradation By ...mentioning

confidence: 98%

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Bacillus subtilis Simultaneously Detoxified Aflatoxin B1 and Zearalenone

Wu,

Wang,

et al. 2024

Applied Sciences

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The co-occurrence of aflatoxin B1 (AFB1) and zearalenone (ZEN) in grain-based food and animal feed poses significant health risks to humans and animals due to their potent mutagenic, cytotoxic, and carcinogenic properties. Conventional physical and chemical methods are insufficient for effectively detoxifying multiple mycotoxins present in food and feed. In this study, we evaluated the capability of Bacillus subtilis ZJ-2019-1 (B. subtilis ZJ-2019-1) to simultaneously degrade AFB1 and ZEN while optimizing reaction to enhance degradation efficiency. The localization of active ingredients from B. subtilis ZJ-2019-1 was determined using high-performance liquid chromatography (HPLC). Our findings demonstrated that B. subtilis ZJ-2019-1 eliminated 60.88% of AFB1 and 33.18% of ZEN within 72 h at a concentration of 10 mg/L at 37 °C (pH 7.0) and exerted greater activity under alkaline conditions. The autoclaved and boiled supernatants of B. subtilis ZJ-2019-1 exhibited significant enhancement in the degradation of AFB1 and ZEN, achieving degradation rates of 79.85% and 100%, respectively, at a concentration of 1 mg/L within 48 h at 37 °C. Moreover, the crude enzymes from B. subtilis ZJ-2019-1 showed maximum degradation rates for AFB1 (100%) and ZEN (94.29%) within 72 h at 70 °C. Additionally, divalent cations (such as Co2+, Fe2+, Mn2+, and Ni2+) significantly augmented the activity of crude enzymes from B. subtilis ZJ-2019-1 towards mycotoxin degradation. Furthermore, when applied to corn gluten meals, B. subtilis ZJ-2019-1 strain effectively detoxify 66.08% of AFB1 and 22.01% of ZEN, surpassing the efficacy of a commercial detoxification agent on the market (34.17% for AFB1 and 2.28% for ZEN). Collectively, these findings indicated that B. subtilis ZJ-2019-1 is a promising candidate for the simultaneous removal of multiple mycotoxins in food and feed, while addressing health concerns associated with harmful mycotoxins.

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Section: Effects Of Different Factors On Afb1 and Zen Degradation By ...mentioning

confidence: 98%

Section: Effects Of Different Factors On Afb1 and Zen Degradation By ...mentioning

confidence: 98%

Bacillus subtilis Simultaneously Detoxified Aflatoxin B1 and Zearalenone

Wu,

Wang,

et al. 2024

Applied Sciences

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“…Cytobacillus oceanisediminis, detected on dispensers by the university's department of histology, and Paenibacillus glucanolyticus, by the university's department of hygiene, scored low-confidence identification values. They are mainly isolated from marine sediments and plants and no reference to HAIs has been reported so far [69,70].…”

Section: Discussionmentioning

confidence: 99%

Microbial Load of Hand Sanitizer Dispensers—A University Hospital Study

Stefanis,

Giorgi,

Stavropoulou

et al. 2023

Hygiene

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Hospital-acquired infections are a significant concern in healthcare settings, leading to patient safety risks, increased morbidity and mortality, and financial burdens. Hand hygiene is crucial in preventing the spread of bacteria in hospitals and communities. Manual hand sanitizer dispensers can harbor presumptive pathogenic bacteria and act as fomites for bacterial transmission. This study aimed to assess the microbial contamination of manual hand sanitizer dispensers in a hospital setting and to study their antibiotic resistance profiles. Samples were collected using sterile cotton swabs and then inoculated into brain heart infusion broth. Subsequent subcultures were performed on both blood and MacConkey agar. The isolates were then identified using the Bruker MALDI Biotyper (Bruker Daltonik, Bremen, Germany) to the species level. Sampling was conducted in various wards and in the hospital and the University areas on dispenser levers and nozzle areas. The results showed that all samples yielded one or more bacterial species. Bacterial isolates identified belonged to species commonly found on the skin microflora and some Gram-negative enteric bacilli. Higher colonization was observed on the dispenser lever. Among Gram+ microorganisms, most bacterial species were shown to be sensitive to β-lactams, with the exception of Staphylococcus spp., resistant to AMP (Ampicillin) and Penicillin. However, no Methicillin resistant isolates were detected. Gram− microorganisms such as Pseudomonas luteola were shown to be sensitive to all tested antibiotics, while Pantoea agglomerans was shown to be resistant to AMC (amoxicillin–clavulanic acid). Rifampicin tested only against Bacilli showed resistance. Based on the findings, it is recommended to implement systematic cleaning and proper maintenance of manual dispenser areas or to use automated dispensers to reduce hand contact and minimize microbial contamination. Monitoring the presence of microorganisms in hand sanitizing gels and dispensers is an essential infection control strategy.

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“…The filamentous fungi such as Aspergillus niger GX312, A. japonicus AX35, Aspergillus carbonarius SA332 (225), in addition to A. fumigatus, A. clavatus, A. ochraceus, A. versicolor, A. wentii, Cladosporium sp., P. aurantiogriseum, P. spinulosum and Botrytis cinerea (isolated from grapes) are also reported to degrade OTA (227)(228)(229). In addition, some yeast strains such as Yarrowia lipolytica and Yarrowia lipolytica Y-2 are also reported to be effective in OTA degrading (223,239). The mechanism of OTA degradation is mainly by its detoxification to the non-toxic metabolite OTα through the hydrolysis of an amide bond via hydrolytic enzymes, such as carboxypeptidase A, carboxypeptidase PJ_1540, protease A, lipase A, ochratoxinase, etc.…”

Section: Mycotoxin Detoxification By Biotransformation or Bindingmentioning

confidence: 99%

Natural feed additives and bioactive supplements versus chemical additives as a safe and practical approach to combat foodborne mycotoxicoses

Stoev

2024

Front. Nutr.

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This review highlights the possible hazard of mycotoxins occurrence in foods and feeds in regards to foodborne diseases. The possible management of the risk of contamination of foods and feeds with mycotoxins by using natural feed additives, protecting against deleterious effects of mycotoxins or inhibiting the growth of fungi and mycotoxin production, is deeply investigated in the available literature and some effective measures for safe utilization of mycotoxin contaminated feed/food are proposed. The biological methods of decontamination, degradation or biotransformation of mycotoxins are deeply analyzed and discussed. Some natural antagonists against target fungi are also reviewed and a comparison is made with conventional fungicides for ensuring a safe prevention of mycotoxin contamination. The most common and useful chemical methods of mycotoxins decontamination of agricultural commodities or raw materials are also investigated, e.g., chemical additives inactivating or destroying and/or adsorbing mycotoxins as well as chemical additives inhibiting the growth of fungi and mycotoxin production. The practical use and safety of various kind of feed/food additives or herbal/biological supplements as possible approach for ameliorating the adverse effects of some dangerous mycotoxins is deeply investigated and some suggestions are given. Various possibilities for decreasing mycotoxins toxicity, e.g., by clarifying the mechanisms of their toxicity and using some target antidotes and vitamins as supplements to the diet, are also studied in the literature and appropriate discussions or suggestions are made in this regard. Some studies on animal diets such as low carbohydrate intake, increased protein content, calorie restriction or the importance of dietary fats are also investigated in the available literature for possible amelioration of the ailments associated with mycotoxins exposure. It could be concluded that natural feed additives and bioactive supplements would be more safe and practical approach to combat foodborne mycotoxicoses as compared to chemical additives.

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Isolation and identification of a Cytobacillus oceanisediminis strain with ochratoxin A detoxification ability

Cited by 8 publications

References 49 publications

Bacillus subtilis Simultaneously Detoxified Aflatoxin B1 and Zearalenone

Bacillus subtilis Simultaneously Detoxified Aflatoxin B1 and Zearalenone

Microbial Load of Hand Sanitizer Dispensers—A University Hospital Study

Natural feed additives and bioactive supplements versus chemical additives as a safe and practical approach to combat foodborne mycotoxicoses

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