Isolation and identification of a staphylococal strain with an anti-mycobacterial activity and study of it’s mode of action

Hassi, Mohammed; Haggoud, Abdellatif; Mzibri, Mohammed El; Koraichi, Saâd Ibnsouda; Houari, Abdellah; Iraqui, Mohammed

doi:10.1007/bf03175368

Cited by 10 publications

(14 citation statements)

References 46 publications

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“…Growth conditions were chosen according to the experimental requirements. Mycobacterial strains were grown at 37 °C, in sauton medium (monopotassium phosphate, 1.0 g/l; disodium phosphate, 2.5 g/l; magnesium sulfate, 0.01 g/l; zinc sulfate, 0.001 g/l; copper sulfate, 0.001 g/l; ferric ammonium citrate, 0.05 g/l; Casitone 2.0 g/l; asparagine, 5.0 g/l; and glycerol, 10.0 g/l) [20,21].…”

Section: Bacterial Strains Plasmids Media and Growth Conditionsmentioning

confidence: 99%

Lycopene Production in Mycobacterium Smegmatis by Expression of CRT Genes From Mycobacterium Aurum and Protective Effect of Lycopene in Vivo and in Vitro Against Uv Radiation

Elamin

Chraibi

Koraichi

et al. 2018

Int J Pharm Pharm Sci

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Objective: The aim of the present study was to express in Mycobacterium smegmatis the clustered mycobacterial genes coding for lycopene synthesis and to investigate the protective properties of lycopene against ultraviolet (UV) irradiation.Methods: The genes, which encode the biogenesis of lycopene in Mycobacterium aurum A+, were introduced into Mycobacterium smegmatis by electroporation. The pigments produced were analyzed by thin layer chromatography, and the absorption spectra were determined. A survival test using UV irradiations was also performed.Results: The transformed Mycobacterium smegmatis were found to synthesize lycopene with important yield (1.41± 3.09 mg/g) and was more resistant to ultraviolet irradiation than non-pigmented strain (p<0.01). Furthermore, cells of M. smegmatis not transformed but coated with lycopene are more resistant to UV than those uncoated (p<0.01).Conclusion: M. smegmatis can form orange colonies on agar plates when it is transformed with the lycopene genes, and the transformants produces 1.41 mg/g (dry weight) of this carotene. Our findings strongly suggest that lycopene has antioxidant activities and prevent the lethal action of UV irradiation on bacterial cells in vivo and in vitro, and deserves further studies considering the amelioration of the production.

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Section: Bacterial Strains Plasmids Media and Growth Conditionsmentioning

confidence: 99%

Lycopene Production in Mycobacterium Smegmatis by Expression of CRT Genes From Mycobacterium Aurum and Protective Effect of Lycopene in Vivo and in Vitro Against Uv Radiation

Elamin

Chraibi

Koraichi

et al. 2018

Int J Pharm Pharm Sci

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“…The supernatant was recovered after sedimentation of the heavier particles. Serial dilutions (of 10 -1 to 10 -5 ) were carried out in sterile Luria Bertoni (LB) broth (Hassi et al, 2007) (peptone: 10 g/l; yeast extract: 5 g/l; sodium chloride: 10 g/l). An aliquot of 70 µl of each dilution was spread on LB-agar previously inoculated with 70 µl of M. aurum A + or M. smegmatis cultures having 0.3 optical density at 600 nm (OD600nm).…”

Section: Isolation Of a Bacterial Strain With An Antimycobacterial Efmentioning

confidence: 99%

“…In order to get an idea on the activity spectrum, the antimicrobial effect of the isolate under study was evaluated against the following bacteria: M. aurum A + ; M. smegmatis MC 2 155; E. coli DH5α; Bacillus subtilis CIP 5262; Staphylococcus haemolyticus (Hassi et al, 2007).…”

Section: Activity Spectrum Of the Isolate (S3)mentioning

confidence: 99%

Isolation of a new Staphylococcus epidermidis strain with anti-mycobacterial activity and determination of the nature of its active substance

Guendouzi¹,

Hassi²,

David³

et al. 2013

Afr. J. Microbiol. Res.

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Tuberculosis is the second cause of death from infectious disease. The resurgence of tuberculosis and the increase in mycobacterial infections, as well as the multidrug-resistance of these bacteria to available antibiotics, has encouraged research on new antimycobacterial agents. In this context, water and soil samples from different Moroccan biotopes have led to the isolation of a bacterial strain, capable of inhibiting mycobacterial growth (Mycobacterium smegmatis MC 2 155 and Mycobacterium aurum A + ). This effect was found to be due to a secreted substance. 16S RNAr gene sequencing identified the isolate as Staphylococcus epidermidis. The biologically active substance from this strain was extracted using ethyl acetate. The precipitate was not sensitive to proteinase K and therefore not a protein. Analysis of the ethyl acetate extract by thin layer chromatography allowed the identification of the antimycobacterial fraction. A phytochemical study identified polyphenol in the bioactive fraction responsible for the observed antimycobacterial agents.

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“…In order to get an idea on the spectrum of activity, the antimicrobial effect of the isolates under study was evaluated against the following bacteria: Mycobacterium aurum A + , Mycobacterium smegmatis MC 2 155, Escherichia coli DH5α, Bacillus subtilis (EL Guendouzi et al, 2011), Staphylococcus haemolyticus (Hassi et al, 2007). The test was repeated three times for each strain.…”

Section: Activity Spectrum Of the Isolatementioning

confidence: 99%

Isolation and identification of a Staphylococcus warneri strain with anti-mycobacterial activity

Guendouzi¹,

Haouat²,

David³

et al. 2013

Afr. J. Biotechnol.

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Tuberculosis is the principal cause of death from infection in the world. The resurgence of tuberculosis and the increase in mycobacterial infections, as well as multidrug-resistance of mycobacteria to available antibiotics, has incentivized research on new antimycobacterial agents. Therefore, research based on water and soil samples from the Moroccan biotopes, has led to the isolation of a bacterial strain capable of inhibiting mycobacterial growth (Mycobacterium smegmatis and Mycobacterium aurum A +). The effect was due to an active substance secreted into the culture medium. Sequencing of the 16S rRNA gene identified the strain as belonging to the species Staphylococcus warneri. The active substance precipitated using ammonium sulfate, maintained its inhibitory properties, which were lost when treated with proteinase K. These results indicated that the active substance was protein. Study of the activity of the metabolite revealed its effect on M. smegmatis cell wall, facilitating genomic DNA extraction.

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Isolation and identification of a staphylococal strain with an anti-mycobacterial activity and study of it’s mode of action

Cited by 10 publications

References 46 publications

Lycopene Production in Mycobacterium Smegmatis by Expression of CRT Genes From Mycobacterium Aurum and Protective Effect of Lycopene in Vivo and in Vitro Against Uv Radiation

Lycopene Production in Mycobacterium Smegmatis by Expression of CRT Genes From Mycobacterium Aurum and Protective Effect of Lycopene in Vivo and in Vitro Against Uv Radiation

Isolation of a new Staphylococcus epidermidis strain with anti-mycobacterial activity and determination of the nature of its active substance

Isolation and identification of a Staphylococcus warneri strain with anti-mycobacterial activity

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