Isolation and Identification of a 10-Deacetyl Baccatin-III-Producing Endophyte from Taxus wallichiana

Li, Yongchao; Yang, Jing; Zhou, Xiuren; Wang, Zhao; Zaiyou, Jian

doi:10.1007/s12010-014-1422-0

Cited by 24 publications

(10 citation statements)

References 15 publications

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“…The genus Taxus is composed of the well-known source plants of anticancer paclitaxel (Taxol) and other useful taxanes 14 15 . Taxus associated microbes not only are involved in the taxane biosynthesis 16 17 18 19 and the biotransformation of taxane compounds 2 , but also have other functional potentials. For instance, the phosphate-solubilizing bacteria were isolated from T. mairei rhizosphere 20 , which could be used to improve the growth of the seedlings.…”

Section: Discussionmentioning

confidence: 99%

Unearthing microbial diversity of Taxus rhizosphere via MiSeq high-throughput amplicon sequencing and isolate characterization

Hao

Song

et al. 2016

Sci Rep

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The species variability and potential environmental functions of Taxus rhizosphere microbial community were studied by comparative analyses of 15 16S rRNA and 15 ITS MiSeq sequencing libraries from Taxus rhizospheres in subtropical and temperate regions of China, as well as by isolating laccase-producing strains and polycyclic aromatic hydrocarbon (PAH)-degrading strains. Total reads could be assigned to 2,141 Operational Taxonomic Units (OTUs) belonging to 31 bacteria phyla and 2,904 OTUs of at least seven fungi phyla. The abundance of Planctomycetes, Actinobacteria, and Chloroflexi was higher in T. cuspidata var. nana and T. × media rhizospheres than in T. mairei rhizosphere (NF), while Acidobacteria, Proteobacteria, Nitrospirae, and unclassified bacteria were more abundant in the latter. Ascomycota and Zygomycota were predominant in NF, while two temperate Taxus rhizospheres had more unclassified fungi, Basidiomycota, and Chytridiomycota. The bacterial/fungal community richness and diversity were lower in NF than in other two. Three dye decolorizing fungal isolates were shown to be highly efficient in removing three classes of reactive dye, while two PAH-degrading fungi were able to degrade recalcitrant benzo[a]pyrene. The present studies extend the knowledge pedigree of the microbial diversity populating rhizospheres, and exemplify the method shift in research and development of resource plant rhizosphere.

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Section: Discussionmentioning

confidence: 99%

Unearthing microbial diversity of Taxus rhizosphere via MiSeq high-throughput amplicon sequencing and isolate characterization

Hao

Song

et al. 2016

Sci Rep

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“…Besides a number of papers where these precursors are reported together with taxol (Table 3), specific references consider their production by endophytic strains from Taxus wallichiana, respectively Diaporthe phaseolorum [278] and Trichoderma sp. [279]. A more general biosynthetic ability concerning taxanes has been evidenced in a number of endophytic strains isolated from Taxus baccata which were not identified at the species level, ascribed to the genera Alternaria, Aspergillus, Beauveria, Epicoccum, Fusarium, Gelasinospora, Geotrichum, Phoma and Phomopsis [280].…”

Section: Speciosus T Chinensismentioning

confidence: 99%

Plant Bioactive Metabolites and Drugs Produced by Endophytic Fungi of Spermatophyta

2015

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Abstract:It is known that plant-based ethnomedicine represented the foundation of modern pharmacology and that many pharmaceuticals are derived from compounds occurring in plant extracts. This track still stimulates a worldwide investigational activity aimed at identifying novel bioactive products of plant origin. However, the discovery that endophytic fungi are able to produce many plant-derived drugs has disclosed new horizons for their availability and production on a large scale by the pharmaceutical industry. In fact, following the path traced by the blockbuster drug taxol, an increasing number of valuable compounds originally characterized as secondary metabolites of plant species belonging to the Spermatophyta have been reported as fermentation products of endophytic fungal strains. Aspects concerning sources and bioactive properties of these compounds are reviewed in this paper.

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“…The versatile utility of Taxus associated microbes has been illustrated . Taxus associated microbes not only are involved in the taxane biosynthesis and the biotransformation of taxanes and aromatic compounds , but also have other functional potentials.…”

Section: Introductionmentioning

confidence: 99%

The first Taxus rhizosphere microbiome revealed by shotgun metagenomic sequencing

2018

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In the present study, the shotgun high throughput metagenomic sequencing was implemented to globally capture the features of Taxus rhizosphere microbiome. Total reads could be assigned to 6925 species belonging to 113 bacteria phyla and 301 species of nine fungi phyla. For archaea and virus, 263 and 134 species were for the first time identified, respectively. More than 720,000 Unigenes were identified by clean reads assembly. The top five assigned phyla were Actinobacteria (363,941 Unigenes), Proteobacteria (182,053), Acidobacteria (44,527), Ascomycota (fungi; 18,267), and Chloroflexi (15,539). KEGG analysis predicted numerous functional genes; 7101 Unigenes belong to "Xenobiotics biodegradation and metabolism." A total of 12,040 Unigenes involved in defense mechanisms (e.g., xenobiotic metabolism) were annotated by eggNOG. Talaromyces addition could influence not only the diversity and structure of microbial communities of Taxus rhizosphere, but also the relative abundance of functional genes, including metabolic genes, antibiotic resistant genes, and genes involved in pathogen-host interaction, bacterial virulence, and bacterial secretion system. The structure and function of rhizosphere microbiome could be sensitive to non-native microbe addition, which could impact on the pollutant degradation. This study, complementary to the amplicon sequencing, more objectively reflects the native microbiome of Taxus rhizosphere and its response to environmental pressure, and lays a foundation for potential combination of phytoremediation and bioaugmentation.

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Isolation and Identification of a 10-Deacetyl Baccatin-III-Producing Endophyte from Taxus wallichiana

Cited by 24 publications

References 15 publications

Unearthing microbial diversity of Taxus rhizosphere via MiSeq high-throughput amplicon sequencing and isolate characterization

Unearthing microbial diversity of Taxus rhizosphere via MiSeq high-throughput amplicon sequencing and isolate characterization

Plant Bioactive Metabolites and Drugs Produced by Endophytic Fungi of Spermatophyta

The first Taxus rhizosphere microbiome revealed by shotgun metagenomic sequencing

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