Isolation and identification of Acanthamoeba species from natural water sources in the northeastern part of Thailand

Thammaratana, Thani; Laummaunwai, Porntip; Boonmars, Thidarut; Neglected, zoonosis

doi:10.1007/s00436-016-4911-y

Cited by 11 publications

(7 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Delafont et al (2013) reported Acanthamoeba from municipal water sources in France. Furthermore, the isolation of Acanthamoeba was also documented in the Gilan region in Iran's surface water (lagoons, lakes and rivers) and freshwater resources (Mahmoudi et al 2015), in natural water samples from 11 provinces in Northeast Thailand (Thammaratana et al 2016), in recreational hot springs from Malaysia (Mohd Hussain et al 2019) and two major water reservoirs in the Philippines (Milanez et al 2020a), including a nationwide survey of Acanthamoeba species and genotypes in various freshwater systems from the Philippines (Milanez et al 2020b).…”

Section: Discussionmentioning

confidence: 97%

Survey and first report ofAcanthamoebaT4 genotype in natural spring water resources in the Black Sea, Turkey

Karaman

Kolören

Karanis

2021

Journal of Water and Health

View full text Add to dashboard Cite

Infection with Acanthamoeba spp. may result in granulomatous amoebic encephalitis and Acanthamoeba keratitis. Water is an important habitat where Acanthamoeba species thrive. Therefore, studying the occurrence of this free-living amoeba in water sources will help understand the infection dynamics. The aim of the study was to survey and report on the presence of Acanthamoeba spp. in water resources from the Ordu and Giresun provinces in Black Sea. Acanthamoeba spp. was found in 1/17 natural spring water samples from Ordu and in 2/18 from Giresun. Acanthamoeba species were not detected in any of the investigated tap water samples. Sequencing of the (SSU) rDNA gene resulted in the identification of haplotype I (Acanthamoeba genotype: KJ094684). T4 (8.6%) was the only isolated genotype in both Ordu and Giresun provinces. This is the first report of Acanthamoeba T4 genotype in natural spring water resources in the Black Sea. The occurrence of Acanthamoeba species in natural spring water sources should be considered as a potential risk for human infection, especially to high-risk populations.

show abstract

Section: Discussionmentioning

confidence: 97%

Survey and first report ofAcanthamoebaT4 genotype in natural spring water resources in the Black Sea, Turkey

Karaman

Kolören

Karanis

2021

Journal of Water and Health

View full text Add to dashboard Cite

show abstract

“…However, incidence of AK in developing countries is often related with outdoor activities and risk exposures to dust, soil or water [1]. In Thailand, Acanthamoeba have been isolated from clinical and environmental samples [5,16–19]. Despite its ubiquity in the nature, less than 10 AK cases have been previously reported from Thailand [20], bringing up to the curiosity that the true AK incidence may be underestimated.…”

Section: Discussionmentioning

confidence: 99%

“…The clustering of the MU_AK into T4 monophyletic clade, could be explained that the 0.73% of genetic distances were remained lower than the 5% cut off value which is known to be characteristic of different genotype [11]. To our knowledge, few molecular studies of Acanthamoeba isolated in Thailand have been documented [18,19,36,37]. Clinically, genotypes T4 and T10 were reported to be responsible for Acanthamoeba keratitis cases, while only T4 was identified from a case of Acanthamoeba meningoencephalitis [18].…”

Section: Discussionmentioning

confidence: 99%

“…Clinically, genotypes T4 and T10 were reported to be responsible for Acanthamoeba keratitis cases, while only T4 was identified from a case of Acanthamoeba meningoencephalitis [18]. Environmentally, genotypes T3, T4, T5, T9, and T17 were isolated from natural water resources of Thailand [18,19]. It is likely that T4 is the most widespread distribution genotype in the country.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The First Acanthamoeba keratitis Case of Non-Contact Lens Wearer with HIV Infection in Thailand

et al. 2019

View full text Add to dashboard Cite

Acanthamoeba keratitis (AK) is a rare sight-threatening corneal infection, often reporting from contact lens wearers. An asymptomatic human immunodeficiency virus (HIV)-infected Thai male without history of contact lens use complained foreign body sensation at his left eye during motorbike riding. He had neither specific keratitis symptoms nor common drugs responding, which contributed to delayed diagnosis. By corneal re-scraping, Acanthamoeba-like cysts were detected by calcofluor white staining and agar culture. The etiological agent obtained from the culture was molecularly confirmed by Acanthamoeba spp.-specific PCR, followed by DNA sequencing. The results from BLAST and phylogenetic analysis based on the DNA sequences, revealed that the pathogen was Acanthamoeba T4, the major genotype most frequently reported from clinical isolates. The infection was successfully treated with polyhexamethylene biguanide resulting in corneal scar. This appears the first reported AK case from a non-contact lens wearer with HIV infection in Thailand. Although AK is sporadic in developing countries, a role of free-living Acanthamoeba as an opportunistic pathogen should not be neglected. The report would increase awareness of AK, especially in the case presenting unspecific keratitis symptoms without clinical response to empirical antimicrobial therapy.

show abstract

“…Many studies have recorded the wide distribution in soil and water, with differing range of thermal tolerance (Table 1). They have been isolated in untreated natural freshwaters, like lakes, ponds, hot springs and waterfalls [14][15][16][17]; and brackish, seawaters, and ocean sediments [18]. They were also isolated from treated waters like domestic water systems, swimming pools, hydrotherapy pools, remedial spas, tap water and drinking water [14,16,19,20].…”

Section: Epidemiology Of Acanthamoeba In Aseansmentioning

confidence: 99%

Acanthamoeba in Southeast Asia – Overview and Challenges

et al. 2019

View full text Add to dashboard Cite

Acanthamoeba, one of free-living amoebae (FLA), remains a high risk of direct contact with this protozoan parasite which is ubiquitous in nature and man-made environment. This pathogenic FLA can cause sight-threatening amoebic keratitis (AK) and fatal granulomatous amoebic encephalitis (GAE) though these cases may not commonly be reported in our clinical settings. Acanthamoeba has been detected from different environmental sources namely; soil, water, hot-spring, swimming pool, air-conditioner, or contact lens storage cases. The identification of Acanthamoeba is based on morphological appearance and molecular techniques using PCR and DNA sequencing for clinico-epidemiological purposes. Recent treatments have long been ineffective against Acanthamoeba cyst, novel anti-Acanthamoeba agents have therefore been extensively investigated. There are efforts to utilize synthetic chemicals, lead compounds from medicinal plant extracts, and animal products to combat Acanthamoeba infection. Applied nanotechnology, an advanced technology, has shown to enhance the anti-Acanthamoeba activity in the encapsulated nanoparticles leading to new therapeutic options. This review attempts to provide an overview of the available data and studies on the occurrence of pathogenic Acanthamoeba among the Association of Southeast Asian Nations (ASEAN) members with the aim of identifying some potential contributing factors such as distribution, demographic profile of the patients, possible source of the parasite, mode of transmission and treatment. Further, this review attempts to provide future direction for prevention and control of the Acanthamoeba infection.

show abstract

Isolation and identification of Acanthamoeba species from natural water sources in the northeastern part of Thailand

Cited by 11 publications

References 17 publications

Survey and first report ofAcanthamoebaT4 genotype in natural spring water resources in the Black Sea, Turkey

Survey and first report ofAcanthamoebaT4 genotype in natural spring water resources in the Black Sea, Turkey

The First Acanthamoeba keratitis Case of Non-Contact Lens Wearer with HIV Infection in Thailand

Acanthamoeba in Southeast Asia – Overview and Challenges

Contact Info

Product

Resources

About