Isolation and identification of Acanthamoeba species related to amoebic encephalitis and nonpathogenic free-living amoeba species from the rice field

Liang, S.-Y.; Ji, Dar-Der; Hsia, Kan Tai; Hung, Chien‐Ching; Sheng, Wang-Huei; Hsu, Bing-Mu; Chen, Jung‐Sheng; Wu, Mei‐Zhen; Lai, Chao-Hung; Ji, Dar Der

doi:10.1111/j.1365-2672.2010.04779.x

Cited by 33 publications

(16 citation statements)

References 33 publications

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“…This heterologous recognition may be caused by the relative proximity of these species in evolutionary terms. Such proximity is shown by phylogenetic analysis …”

Section: Discussionmentioning

confidence: 86%

Experimental keratitis in rats caused by Acanthamoeba griffini: A kinetic histopathological study

Ávila-Blanco

Martín-Pérez

Ventura‐Juárez

et al. 2020

Parasite Immunology

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The aim of this study was to evaluate the inflammation process that resulted from the inoculation of Wistar Rats with Acanthamoeba griffini, a virulent T3 Acanthamoeba genotype that produces keratitis. Haematoxylin and eosin, periodic acid stain, immunohistochemistry and morphometry were used to analyse tissues from rats of an Acanthamoeba keratitis (AK) model. Two weeks after inoculating the rats with A griffini trophozoites, the thickness of the stroma had diminished, followed by an increase in thickness at 4 weeks. At the latter time, an abundance of inflammatory infiltrate cells was observed, some found to express IL‐1β, IL‐10 and/or caspase 3. Intercellular adhesion molecule‐1 was expressed in corneal blood vessels amid the abundant vascularization characteristic of the development of AK. Through an immunohistochemical technique, trophozoites were detected at 2 and 4 weeks post‐inoculation. By 8 weeks, there were a low number of trophozoites and cysts and the corneas of infected rats were similar in thickness to those of the controls. Thus, the rats were capable of healing experimental AK in the present rat model. Diverse immunological mechanisms regulated the inflammatory process in acute AK induced by A griffini in a murine model.

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“…This heterologous recognition may be caused by the relative proximity of these species in evolutionary terms. Such proximity is shown by phylogenetic analysis …”

Section: Discussionmentioning

confidence: 86%

Experimental keratitis in rats caused by Acanthamoeba griffini: A kinetic histopathological study

Ávila-Blanco

Martín-Pérez

Ventura‐Juárez

et al. 2020

Parasite Immunology

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“…The present study is the first comprehensive investigation on surface and tap water and also indoor swimming pools of Shiraz City which has been conducted to identify the pathogenic free-living amoeba by molecular method. Infections owing to Acanthamoeba have been increased worldwide recently, which is more likely related to the presence of Acanthamoeba in the natural environment, especially in water sources (Liang et al, 2010). Previous study looking at various samples revealed that medical instrumentation, air conditioners, windows, floors, sinks, water taps, bathrooms, and showers could be a possible source of Acanthamoeba in Iran (Lasjerdi et al, 2011).…”

Section: Discussionmentioning

confidence: 97%

Isolation and identification of pathogenic free-living amoeba from surface and tap water of Shiraz City using morphological and molecular methods

2015

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Free-living amoebae (FLA) are the most abundant and widely distributed protozoa in the environment. An investigation was conducted to determine the presence of free-living amoebae (FLA), Acanthamoeba and Vermamoeba in waterfronts of parks and squares and tap water of Shiraz City, Iran. FLA are considered pathogenic for human. These ubiquitous organisms have been isolated from different environments such as water, soil, and air. Eighty-two water samples were collected from different places of Shiraz City during the summer of 2013. All samples were processed in Dept. of Parasitology and Mycology, Shiraz University of Medical Sciences, Fars, Iran. Samples were screened for FLA and identified by morphological characters in the cultures, PCR amplification targeting specific genes for each genus and sequencing determined frequent species and genotypes base on NCBI database. Overall, 48 samples were positive for Acanthamoeba and Vermamoeba in non-nutrient agar culture based on morphological characteristics. The PCR examination was done successfully. Sequencing results were revealed T4 (62.96 %) genotypes as the most common genotype of Acanthamoeba in the Shiraz water sources. In addition, T5 (33.33 %) and T15 (3.71 %) were isolated from water supplies. Vermamoeba vermiformis was known the dominant species from this genus. The high frequency of Acanthamoeba spp. and Vermamoeba in different environmental water sources of Shiraz is an alert for the public health related to water sources. The result highlights a need for taking more attention to water supplies in order to prevent illnesses related to free-living amoebae.

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“…The protistan genus Acanthamoeba comprises several species of the opportunistic and pathogenic free-living amoebae, widespread in many types of environments and playing a role of major microbial predators (Corsaro and Venditti 2010;Liang et al 2010). The protistan genus Acanthamoeba species have been classified into three distinct morphological groups based largely on the cyst morphology of the species and encompassing more than 25 nominal species (Booton et al 2005;Visvesvara and Schuster 2008a, b).…”

Section: Introductionmentioning

confidence: 98%

Molecular detection and comparison of Acanthamoeba genotypes in different functions of watersheds in Taiwan

Kao

Hsu

Chen

et al. 2011

Environ Monit Assess

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The protistan genus Acanthamoeba is a free-living amoeba existing in various environments. Within this protistan genus, there are some species recognized as potential human pathogens, which may cause granulomatous amoebic encephalitis, Acanthamoeba keratitis and chronic granulomatous lesions of the skin. In this study, 211 water samples were collected from two watersheds in southern Taiwan. We detected Acanthamoeba based on the PCR amplification with a genus-specific primer pair and investigation of Acanthamoeba in Puzih River and Kaoping River in southern Taiwan. Acanthamoeba species were detected in 34 (16.1%) samples. The presence or absence of Acanthamoeba within the water samples showed significant difference with the levels of water temperature and total coliforms. The most frequently identified Acanthamoeba genotype was T4 (n = 19), followed by T5 (n = 8), and then T15 (n = 3). Genotype T6, T7/T8, T11 and T12 were all detected once. Genotype T4, T5, T6, T11 and T15 of Acanthamoeba are responsible for Acanthamoeba keratitis and should be considered a potential health threat associated with human activities in environmental surface water watersheds.

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Isolation and identification of Acanthamoeba species related to amoebic encephalitis and nonpathogenic free-living amoeba species from the rice field

Cited by 33 publications

References 33 publications

Experimental keratitis in rats caused by Acanthamoeba griffini: A kinetic histopathological study

Experimental keratitis in rats caused by Acanthamoeba griffini: A kinetic histopathological study

Isolation and identification of pathogenic free-living amoeba from surface and tap water of Shiraz City using morphological and molecular methods

Molecular detection and comparison of Acanthamoeba genotypes in different functions of watersheds in Taiwan

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