Isolation and Identification of Aeromonas sp. from Fishes

Ganesan, Mirunalini; Mani, Ravi; Sai, Sakthinarenderan

doi:10.1007/978-1-0716-3032-7_1

Cited by 3 publications

(3 citation statements)

References 8 publications

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“…Portions of the skin, gills, liver, heart, and spleen were weighed aseptically and put in separate labelled kryo bottles containing 20 mL of alkaline peptone water (Oxoid®, UK) as the pre-enrichment broth and incubated at 37 °C for 24 h. Growth in the selective pre-enrichment cultures were transferred with a sterile loop and inoculated onto the selective Aeromonas agar (Oxoid®, UK) supplemented with ampicillin (10mg/L) (Oxoid®, UK) and incubated at 37 ° C for 24 h after which dark green, opaque with dark centres colonies which were presumptive for Aeromonas species were streaked on MacConkeys agar (MCA) plates and incubated at 37 ° C for 24 h for lactose fermentation activity (Austin, 2014). After this, Gram reaction, oxidase and catalase tests were also determined for the presumptive Aeromonas species using standard methods (Austin & Austin, 2016;Ganesan et al, 2023).…”

Section: Aeromonas Isolation and Identification From Fishmentioning

confidence: 99%

Prevalence of Aeromonas species in Clarias gariepinus and water from different culture facilities in fish farms in Kwara State, Nigeria

2023

JoSVAS

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Aeromonas species are associated with diseases on fish farms leading to mortality and economic losses. In order to determine the prevalence and diversity of Aeromonas species from water and Clarias gariepinus cultured in the different holding facilities; five hundred and seventy six Clarias gariepinus fish and one hundred and eight water samples were randomly sampled from fish farms in Kwara State. The samples were clinically and bacteriologically examined and confirmed using Oxiod rapid microbat identification test kits for Gram-negative bacteria, Microbact 24E (MB24E). The overall prevalence of Aeromonas species from water from the different cultured facilities revealed water sampled from earthen ponds had the highest prevalence of 66.67 % followed by concrete tanks with a prevalence of 41.66 % and the least prevalence of 30.56 % was recorded for water in plastic tanks. A similar pattern was recorded in Clarias gariepinus from the different holding facilities showing that earthen ponds had the highest prevalence of 40.10 % when compared with concrete tanks 28.65%, and to plastic tanks 20.83 %. Four varying diversities of Aeromonas species comprising of Aeromonas hydrophila, Aeromonas caviae, Aeromonas veronii biovar sobria, and Aeromonas veronii biovar veronii were isolated from both water and fish from the different holding facilities. From this study, cultured Clarias gariepinus were susceptible to Aeromonas species irrespective of the culture facilities. Therefore, there is need for proper management practices and adherence to biosecurity measures to prevent the outbreak and spread of diseases on the farms.

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Section: Aeromonas Isolation and Identification From Fishmentioning

confidence: 99%

Prevalence of Aeromonas species in Clarias gariepinus and water from different culture facilities in fish farms in Kwara State, Nigeria

2023

JoSVAS

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“…Pada kondisi seperti ini kondisi sistem imunnya berada pada titik terendah, sehingga menjadi sangat mudah terinfeksi oleh bakteri patogen (A. hydrophila). Menempelnya patogen pada permukaan tubuh ikan akan memicu terjadinya luka kecil pada permukaan kulit ikan yang disebabkan oleh aktivitas enzim protease yang dilepaskan oleh patogen pada permukaan kulit ikan (Ganesan et al, 2023). Ukuran luka ini kemudian membesar, sehingga memungkinkan patogen tersebut menyebar dan menginfeksi organ bagian dalam dari inangnya.…”

Section: Aeromonas Hydrophilaunclassified

“…Takahashi et al (2014) melaporkan bahwa protease yang dihasilkan oleh A. hydrophila sangat berperan dalam patogenitasnya, karena enzim ini dapat memecah berbagai protein inang, termasuk struktur ekstraselulernya yang tersusun dari berbagai macam protein (gelatin, fibroektin dan kolagen). Pada A. hydrophila terdapat dua jenis enzim protease ekstraseluler, yaitu protease serin dan elastase (Gao et al, 2018;Ganesan et al, 2023).…”

Section: Pengelompokanunclassified

Role of Catecholamine Hormone to Increase the Virulence of Aeromonas hydrophila

Widiantari,

Ramona,

Julyantoro

2023

metamorfosa

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Extreme environmental conditions have been widely reported to trigger stress in exposed living organisms, and such conditions affect their homeostatics. Sress conditions lead vertebrates to release stress hormones, such as catecholamine Norepinephrin (NE) and Dopamine (Dopamine), which involve the hypothalamus-sympathetic-chromaffin (HSC) axis and the hypothalamus-pituitary-interrenal axis (HPI axis). These two axis systems produce catecholamine hormones (including adrenaline, noradrenaline, and dopamine) and corticosteroid hormones (especially cortisol). The presence of these two groups of hormones in the circulatory system of the hosts can act as a signal for pathogenic bacteria, such as Aeromonas hydrophilla commonly found in aquaculture, to start their infection process. These two types of hormones play a role in the expression of Aeromonas hydrophila genes encoding formation of flagella (allowing bacterial cells to swim rapidly to their host), pilli, toxin production, and activation of biofilm formation process. The stress conditions experienced by fishes (which triggers the secretion of catecholamine hormones) can significantly reduce the fish's immune system to function, and this will be exploited by Aeromonas hydrophila to increase its metabolic rate, growth, and virulence in such stressed fish. Specifically, catecholamine hormones can also act as a signal for the Aeromonas hydrophila to activate the ferric uptake regulator gene (fur gene), allowing this pathogenic bacterium to optimally chelate ions of iron (Fe3+) from its environment, resulting in the excessive growth of the pathogen and an increase in its pathogenicity.

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