ISOLATION AND IDENTIFICATION OF ALOCALSTRAIN OF PROBIOTIC  BACTERIAL ACTOBACILLUS PLANTARUM AND STUDIED THE TOLERANCE ABILITY FOR DIFFERENT LEVELS OF pH

Abdul-Abbas, Sinan J.; Al-Badran, Adnan E.; Al-Sherifi, Hasan R.; Al-Bayyar, Aswan H.

doi:10.33762/bvetr.2016.124330

Cited by 5 publications

(6 citation statements)

References 30 publications

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“…Consequently, they represent bacteria from many taxa but mostly from Lactobacillales in the phylum of Firmicutes as previously described. The most mentioned genera of LAB are Lactobacillus, Pediococcus, Leuconostoc, and Weissella (LPLW), which are phylogenetically close to each other [146] and usually measured together as the Lactobacillus group [147]. Bacterial isolates from cattle vaginal samples have been examined on their properties to develop probiotics to avert or treat uterine infections.…”

Section: Lactic Acid Bacteriamentioning

confidence: 99%

Microflora in the Reproductive Tract of Cattle: A Review

Appiah

Wang

2020

Agriculture

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There are microbial communities in and on the bodies of all multicellular organisms, and this microbiota can have a significant impact on the biology of the host. Most studies have focused on the microbiome of the skin, mouth, and gut, whereas relatively little is known about the reproductive microbiome. From the perspective of the bovine reproductive tract, uterine diseases such as metritis and endometritis are traditionally viewed to result only from interactions occurring between the host animal and pathogens originating from either the environment or ascension from the vagina. This outdated opinion has been refuted by recent advanced studies that propose that, in addition to bacteria colonization through the extrinsic and ascending pathways to the vagina, bacteria can also move from the gut to the uterus, which is also associated with reproductive tract disorders. This has led to the concept of the “endogenous route hypothesis”, which has vital inferences for comprehending the etiology of metritis and endometritis. Furthermore, it has opened up the possibility of developing new prophylactic and therapeutic agents as alternatives to antimicrobial agents. In addition, the unveiling of next-generation sequencing technology makes it more convenient to perform detailed sequencing and analysis of data on the cervical, vaginal, and uterine flora and to further study uncultured bacteria in these niches—most importantly, the cervical niche, which previously was thought to have lower bacterial complexity. Research conducted to date has proven that the composition of microflora in a community varies widely between environmental sites, host niches, and health status. Furthermore, it has also been suggested that the occurrence of endometritis in the dairy and beef cattle reproductive tract is neither casual nor indirect but multifactorial. Whether disturbance in the variety of the microflora in the reproductive tract (dysbiosis) has a role in determining the sensitivity to metritis and endometritis is not yet known. This article outlines the current progress in understanding the microflora with regards to the bovine reproductive tract. The compositions of microflora in various niches of the reproductive tract are briefly elucidated. In addition, the functional role of these microflora communities in the reproductive tract is discussed, with particular emphasis on the association of bacterial flora with reproductive disorders and failures. Finally, prophylaxis and therapeutic approaches based on the new comprehension of the effects of antimicrobials, probiotics, and bacteriophages on the composition of the reproductive tract microflora are also considered.

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Section: Lactic Acid Bacteriamentioning

confidence: 99%

Microflora in the Reproductive Tract of Cattle: A Review

Appiah

Wang

2020

Agriculture

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“…Though shift of principal microorganisms occur during the bovine's ovarian cycle [21]. However, the most mentioned genera of bovine's vagina LAB are Lactobacillus,Pediococcus, Leuconostoc, and Weissella, which are phylogenetically close to each other [22] and usually measured together as the Lactobacillus group [23].…”

Section: Discussionmentioning

confidence: 99%

Dynamics of Vaginal Microbiota During Estrous Cycle in Cows through Metagenomic Approach

Purohit¹,

Patel²,

Gohil³

et al. 2021

Preprint

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Background: Using 16s rRNA sequencing of the V3-V4 hypervariable region, the present study is aimed to check vaginal microbiota diversity throughout different stages of the estrous cycle, with attention to hormonal changes and microorganism diversity. Metagenomic research was conducted on vaginal swab samples obtained from healthy cows' at different stages of the estrous cycle. Results: Total sixteen cows were synchronized with dobble PG regime. Nine cows demonstrated estrus within 96 hours and were eligible for the experiment. Vaginal samples for metagenomics and blood samples for hormonal analysis were collected during estrus, metestrus, diestrus and proestrus of synchronized Estrous cycle. The study's findings revealed that the diestrus phase has a different diversity than the other three estrous cycle phases, implying that hormones affect bacterial diversity. Proteobacteria and Firmicutes are the most abundant phyla at the phylum level, accounting for 94 % of bacterial diversity. Actinobacteriota, Patescibacteria, Cyanobacteria, Bacteroidota, and others are fewer common phyla. Proteobacteria are most common throughout the estrus, metestrus, and proestrus stages of the estrous cycle at the phylum level, there was no discernible distinction between the follicular and luteal phases. After statistical correction, Bacillaceae, Alcaligenes, and Enterobacteriaceae & Morganellacea families are more significant. At the diestrus stage, the Family Enterobacteriaceae is lower than at other stages; otherwise, all statistically significant genera are high at diestrus stages. The luteal phase had higher levels of Micrococcus, Stenotrophomonas, UGC-010, Massilia, and Methylobacillus than the follicular phase, however, statistical analysis revealed no substantial difference between the two phases. Lactobacillus genus is present on two samples including the estrus stage and diestrus stages. Conclusions: This study represents an important step towards the understanding of microbial diversity within different stages of the estrous cycle of the dairy cow. The study results revealed dynamics of metabiota during estrous cycle.

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“…In contrast, 10 µL of PCR products were put into each well of the gels for PCR. Size of the PCR detection result, DNA A 100 bp ladder always follows an electrophoretic run.UV Transilluminator documentation system was used to visualize DNA bands [26,27]. Once the results were obtained, they were examined using geneious software.…”

Section: Gel Electrophoresis and Sample Preparationmentioning

confidence: 99%

Biodegradation of Imidacloprid by the Local Isolate Rhizobium pusense

Ali,

Bayar,

Arif

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Imidacloprid is systemic insecticide (1-[(6-chloro-3-pyridinyl) methyl]-N-nitro-2-imidazolidinimine) and the world’s most widely used has significant efficacy against a broad variety of pests and a unique mode of action by using it spreader and irrigation. The persistence of this pesticide in the soil means that it causes environmental damage that must be cleaned up. In this study collected and identified the best bacteria isolate that breakdown imidacloprid from the Plant Protection Director in Baghdad, which has been using neonicotinoid pesticides for years in their own greenhouse for pest control. Using high-performance liquid chromatography HPLC to measuring the residual concentrations of imidacloprid in MSM media at a concentration of 25 mg/L for 21 days to know the best degrading bacterial isolate for imidacloprid. The best bacterial isolate No.43 was able to degrade 50.2% for 21 days of imidacloprid which was identified as Rhizobium pusense according to morphological, cultural and biochemical tests. Moreover, genetic analysis for the 16S rRNA gene and given a new accession number (OP218458.1.) in the GenBank of NCBI. It could be concluded, from this study that the soil previously contaminated for long periods of time with imidacloprid can be treated by degrading the imidacloprid residues in it by isolated bacteria Rhizobium pusense.

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ISOLATION AND IDENTIFICATION OF ALOCALSTRAIN OF PROBIOTIC BACTERIAL ACTOBACILLUS PLANTARUM AND STUDIED THE TOLERANCE ABILITY FOR DIFFERENT LEVELS OF pH

Cited by 5 publications

References 30 publications

Microflora in the Reproductive Tract of Cattle: A Review

Microflora in the Reproductive Tract of Cattle: A Review

Dynamics of Vaginal Microbiota During Estrous Cycle in Cows through Metagenomic Approach

Biodegradation of Imidacloprid by the Local Isolate Rhizobium pusense

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