Isolation and identification of Anti-Oxidant Fraction from Active Extract of Rhizophora mucronata Poir. Leaves

Fraxinus xanthoxyloides is a perennial shrub belonging to family Oleaceae, traditionally used for malaria, jaundice, pneumonia, inflammation, and rheumatism. Our study is aimed to assess the total phenolics (TPC), flavonoids (TFC), terpenoids contents (TTC) and antioxidant profiling of F. xanthoxyloides methanol bark extract (FXBM) and its fractions, hexane, chloroform, ethyl acetate and aqueous, along with high-performance liquid chromatography with diode-array detection (HPLC-DAD). Further, the antioxidant and pulmonary protective potential was explored against carbon tetrachloride (CCl 4 )-induced CCl4-induced pulmonary tissue damage in rats. The highest TPC, TFC and TTC were found in FXBM (133.29 � 4.19 mg/g), ethyl acetate fraction (279.55 � 10.35 mg/g), and chloroform fraction (0.79 � 0.06 mg/g), respectively. The most potent antioxidant capacity was depicted by FXBM (29.21 � 2.40 μg/mg) and ethyl acetate fraction (91.16 � 5.51 μg/mg). The HPLC-DAD analysis revealed the predominance of gallic, chlorogenic, vanillic and ferulic acid in FXBM. The administration of CCl 4 induced oxidative stress, suppressed antioxidant enzymes' activities including catalase, peroxidase, superoxide dismutase, glutathione peroxidase, glutathione-S-transferase, and glutathione reductase. Further, it increased thiobarbituric acid reactive substances (TBARS) and H 2 O 2 levels, induced DNA injuries and reduced the total protein and glutathione content in lung tissues. The treatment of rats with FXBM restored these biochemical parameters to the normal level. Moreover, the histopathological studies of lung tissues demonstrated that FXBM protected rats' lung tissues from oxidative damage restoring normal lung functions. Thus, F. xanthoxyloides bark extract is recommended as adjuvant therapy as protective agent for patients with lung disorders.

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“…The total terpenoids content in the extract/fractions was estimated following the Palaniyandi et al [61] . method after certain adjustments.…”

Section: Methodsmentioning

confidence: 99%

“…The total terpenoids content in the extract/fractions was estimated following the Palaniyandi et al [61] method after certain adjustments. An aliquot of the tested sample (100 μl) was taken in a test tube, then 1.5 ml of chloroform was added, and the mixture was mixed for 3 min.…”

Section: Estimation Of the Total Terpenoids Content (Ttc)mentioning

confidence: 99%

Antioxidant and Pulmonary Protective Potential of Fraxinus xanthoxyloides Bark Extract against CCl₄‐Induced Toxicity in Rats

Jabeen

Hussain

Rasool

et al. 2023

Chemistry & Biodiversity

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“…Penelitian sebelumnya melaporkan kandungan metabolit sekunder pada mangrove R. apiculata meliputi fenol, alkaloid, flavonoid, tannin, saponin, terpenoid dan steroid (Akasia et al, 2021). Kandungan senyawa golongan fenolik mengindikasikan kemampuan suatu ekstrak sebagai sumber antioksidan yang baik (Palaniyandi et al, 2020). Tujuan dari penelitian ini adalah untuk mengkarakterisasi senyawa bioaktif serta aktivitas potensi antibakteri melawan bakteri MDR dan aktivitas antioksidan ekstrak daun R. apiculata yang berasal dari Teluk Awur, Jepara.…”

Section: Pendahuluanunclassified

“…Senyawa dari golongan fenolik seperti flavonoid, tannin dan kuinon diketahui sebagai agen antioksidan yang sangat baik. Hal ini diperkuat oleh Palaniyandi et al (2020), bahwa senyawa fenolik dari tumbuhan merupakan sumber antioksidan alami yang baik. Flavonoid, tannin dan fenolik dikenal sebagai sumber antioksidan yang kuat karena kemampuannya mendonorkan proton untuk menstabilkan radikal bebas.…”

Section: Hasil Dan Pembahasanunclassified

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Kandungan Senyawa Bioaktif dan Aktivitas Biologis Ekstrak Daun Rhizophora apiculata Asal Perairan Teluk Awur, Jepara

et al. 2022

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Rhizophora apiculata is a type of mangrove that has the ability to adapt to extreme environmental conditions such as temperature, low oxygen levels, and high salinity. This adaptability affects the production of secondary metabolites. Information about the antibacterial activity of this mangrove against MDR (Multi-Drug Resistant) bacteria is still very limited. The content of secondary metabolites produced by mangrove R. apiculata is also expected to affect antioxidant activity against free radicals. The purposes of this study were to examine the presence of bioactive compounds by phytochemical tests and to evaluate the antibacterial activity against MDR bacteria such as Pseudomonas aeruginosa, Escherichia coli, Bacillus cereus, and Bacillus subtilis; and antioxidants property of R. Apiculata leaves that were collected from Teluk Awur, Jepara. The leaves were extracted using the multilevel maceration method with solvent sequence n-hexane, ethyl acetate and methanol. Metabolite finger printing using TLC method was carried out to detect the presence of alkaloids, flavonoids, saponins, phenolics, quinones, steroids, and triterpenoids. The antibacterial test was carried out using agar well diffusion method while the antioxidant test was carried out using the DPPH method. The results of the phytochemical test showed that there were groups of alkaloids and steroids in the n-hexane solvent; alkaloids, phenolics, and steroids in ethyl acetate solvent; as well as alkaloids, flavonoids, phenolics, and saponins in methanol solvents. The results of this study indicate that R. apiculata from Teluk Awur Coastal Waters, Jepara had no potential as an antibacterial against MDR (Multi-Drug Resistant) bacteria, however the methanol extract has the potential to be used as an antioxidant with an IC50 value of 85.999 ppm. The bioautography showed that compounds from the phenol group, flavonoids, triterpenoids and b-carotene pigments acted as antioxidant agents in the leaf extract of R. apiculata. Rhizophora apiculata merupakan salah satu jenis mangrove yang memiliki kemampuan beradaptasi terhadap kondisi lingkungan ekstrem seperti suhu, kadar oksigen rendah dan salinitas tinggi. Kemampuan beradaptasi tersebut mempengaruhi produksi matabolit sekunder. Informasi mengenai kemampuan aktivitas antibakteri mangrove jenis ini melawan bakteri MDR (Multi Drug Resistent) masih sangat terbatas. Kandungan metabolit sekunder yang dihasilkan mangrove R. apiculata ini juga diharapkan dapat mempengaruhi aktivitas antioksidan melawan radikal bebas. Tujuan dari penelitian ini adalah untuk mengkaji kandungan senyawa bioaktif serta aktivitas biologis berupa antibakteri melawan bakteri MDR Pseudomonas aeruginosa, Escherichia coli, Bacillus cereus dan Bacillus subtilis; dan antioksidan dari ekstrak daun mangrove R. apiculata asal perairan Teluk Awur, Jepara. Sampel diekstraksi menggunakan 3 pelarut berbeda (n-heksana, etil asetat dan metanol) dengan metode maserasi bertingkat. Analisis metabolit sidik jari dilakukan menggunakan plat KLT untuk mengetahui kandungan senyawa alkaloid, flavonoid, saponin, fenolik, kuinon, steroid dan triterpenoid. Uji antibakteri dilakukan menggunakan metode sumuran sedangkan uji antioksidan dilakukan menggunakan metode DPPH. Hasil uji fitokimia menunjukkan terdapat golongan senyawa alkaloid dan steroid pada pelarut n-heksana; alkaloid, fenolik dan steroid pada pelarut etil asetat; serta alkaloid, flavonoid, fenolik dan saponin pada pelarut metanol. Hasil penelitian ini mengindikasikan bahwa R. apiculata asal perairan Teluk Awur, Jepara tidak potensial sebagai antibakteri melawan bakteri MDR (Multi Drug Resistent), akan tetapi ekstrak metanol potensial digunakan sebagai antioksidan dengan nilai IC50 85,999 ppm. Tahapan bioautografi menunjukkan bahwa senyawa dari golongan fenol, flavonoid, triterpenoid dan pigmen b-karoten berperan sebagai agen antioksidan pada ekstrak daun R. apiculata.

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Green synthesis of gold nanoparticles using Halymenia pseudofloresii extracts and their antioxidant, antimicrobial, and anti-cancer activities

Palaniyandi

Viswanathan

Prabhakaran

et al. 2023

Biomass Conv. Bioref.

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Isolation and identification of Anti-Oxidant Fraction from Active Extract of Rhizophora mucronata Poir. Leaves

Cited by 4 publications

References 21 publications

Antioxidant and Pulmonary Protective Potential of Fraxinus xanthoxyloides Bark Extract against CCl₄‐Induced Toxicity in Rats

Antioxidant and Pulmonary Protective Potential of Fraxinus xanthoxyloides Bark Extract against CCl₄‐Induced Toxicity in Rats

Kandungan Senyawa Bioaktif dan Aktivitas Biologis Ekstrak Daun Rhizophora apiculata Asal Perairan Teluk Awur, Jepara

Green synthesis of gold nanoparticles using Halymenia pseudofloresii extracts and their antioxidant, antimicrobial, and anti-cancer activities

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Isolation and identification of Anti-Oxidant Fraction from Active Extract of Rhizophora mucronata Poir. Leaves

Cited by 4 publications

References 21 publications

Antioxidant and Pulmonary Protective Potential of Fraxinus xanthoxyloides Bark Extract against CCl4‐Induced Toxicity in Rats

Antioxidant and Pulmonary Protective Potential of Fraxinus xanthoxyloides Bark Extract against CCl4‐Induced Toxicity in Rats

Kandungan Senyawa Bioaktif dan Aktivitas Biologis Ekstrak Daun Rhizophora apiculata Asal Perairan Teluk Awur, Jepara

Green synthesis of gold nanoparticles using Halymenia pseudofloresii extracts and their antioxidant, antimicrobial, and anti-cancer activities

Contact Info

Product

Resources

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Antioxidant and Pulmonary Protective Potential of Fraxinus xanthoxyloides Bark Extract against CCl₄‐Induced Toxicity in Rats

Antioxidant and Pulmonary Protective Potential of Fraxinus xanthoxyloides Bark Extract against CCl₄‐Induced Toxicity in Rats