Isolation and Identification of Antimicrobial Compounds in Brazilian Propolis

Aga, Hajime; Shibuya, Takashi; Sugimoto, Toshiyuki; Kurimoto, Masashi; Nakajima, Shûhei

doi:10.1271/bbb.58.945

Cited by 206 publications

(165 citation statements)

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“…As for sample 2, the composition of the extracts increased qualitatively in proportion to the ethanolic content of the solvent, as well as the relative concentration of most of the components, which is in agreement with the yield of EEP. Of the phenolic compounds identified in the propolis extracts of samples 2 and 3, several have been mentioned in other papers studying the antibiotic, 17,[24][25][26] antioxidant, 27 anti-inflammatory, 28 (Table 4) show that there was a significant increase in yield of EEP between 10 and 30 days, but not between 10 and 20 days, nor between 20 and 30 days. Although this increase was of approximately 10% (w/w) in relation to crude propolis, the phenolic content did not differ significantly between the periods tested.…”

Section: Propolis Extractsmentioning

confidence: 96%

Factors that influence the yield and composition of Brazilian propolis extracts

Cunha¹,

Sawaya²,

Caetano³

et al. 2004

J. Braz. Chem. Soc.

154

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Durante a extração de própolis foram variados alguns fatores para determinar como eles afetam o rendimento e o teor de fenóis totais. Seis amostras diferentes da região sudeste do Brasil foram testadas. Os resultados indicam que os maiores rendimentos de extratos de própolis obtidos por maceração, que também apresentam o maior número de componentes, foram aqueles usando 70% (v/v) ou maior proporção de etanol no solvente. A extração em Soxhlet resultou em um maior rendimento. Não foram observadas diferenças entre extratos de macerações com ou sem luz. Um aumento no rendimento da extração foi observado entre os extratos obtidos por maceração durante 10 e 30 dias, porém o teor de fenóis não variou significativamente nesses extratos. O teor de fenóis totais de todos os extratos variou entre 6,41 e 15,24% mas nenhuma correlação direta foi encontrada com qualquer um dos fatores testados.During the extraction of propolis several factors were varied in order to determine how they affected the yield and phenolic composition of the obtained extracts. Six samples of green propolis from the Southeastern region of Brazil were tested. The results indicate that the highest yield of propolis extracts obtained by maceration, which also had the greatest number of components, were those using 70% (v/v) ethanol or more as a solvent. The Soxhlet extraction procedure resulted in even higher yields. No differences were observed between extracts macerated in the presence or absence of light. An increase in yield was observed between extracts macerated for 10 and 30 days although the phenolic content did not vary significantly. The total phenolic content of all extracts varied from 6.41 to 15.24 % but no direct correlation could be found between any of the factors tested. Keywords: propolis, extraction techniques, high performance liquid chromatography IntroductionPropolis, a resinous substance collected by bees from plants around the hive, is masticated by the bees, salivary enzymes and beeswax added, then applied to the combs and walls of the hive, 1 thereby insulating and reinforcing the hives as well as making the environment aseptic. For beekeepers, propolis is a by-product obtained by scraping the walls of the hives. Crude propolis is composed basically of 55% vegetable resins and balsam, 30% bee wax, 10% essential oil and 5% pollen. 2 Due to its antibiotic and anti-fungal activity, propolis has been used in folk medicine for many centuries 3 and is presently used in health food and various pharmaceutical and cosmetic products such as mouthwash preparations, face creams, lotions and tablets. 1 Traditionally, the fraction soluble in 70% ethanol was extracted and referred to as propolis balsam. 3 In order to avoid the characteristic ethanolic smell and solubility problems of this product, propolis extracts using water 4 or other solvents such as glycerol, edible oils and propylene glycol 5 are presently found on the market. There is no standard either for extractive procedure or composition of products that contain propolis extracts...

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Section: Propolis Extractsmentioning

confidence: 96%

Factors that influence the yield and composition of Brazilian propolis extracts

Cunha¹,

Sawaya²,

Caetano³

et al. 2004

J. Braz. Chem. Soc.

154

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“…Ainda citam que em alguns casos os flavonóides são importantes componentes presentes na própolis brasileira. Aga et al (1994) determinam os compostos prenilados como os maiores constituintes da própolis brasileira e relatam que a atividade antibacteriana destes compostos pode ser aumentada com o aumento do número de resíduos prenil na molécula, como citado por Marcucci et al (2001).…”

Section: Análise Fitoquímicaunclassified

Variabilidade sazonal dos constituintes da própolis vermelha e bioatividade em Artermia salina

Nunes¹,

Galindo²,

Deus³

et al. 2009

Rev. bras. farmacogn.

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RESUMO:A própolis é uma substância resinosa coletada pelas abelhas de diversas partes das plantas. Sua composição depende da época, vegetação e local de coleta. Apresenta diversas atividades biológicas como antimicrobiana, antioxidante, antitumoral, dentre outras. Foi realizado estudo da variabilidade sazonal, nos meses de fevereiro, junho e outubro de 2006, dos constituintes voláteis da própolis vermelha de Pernambuco através da extração por headspace dinâmico e identificação por cromatografia gasosa acoplada com espectrometria de massas (CG-EM). Foram identificados 34 constituintes voláteis, sendo monoterpenos e monoterpenóides, sesquiterpenos e sesquiterpenóides, fenilpropanóides, aldeídos, cetonas e η-alcanos. Os constituintes majoritários foram o trans-anetol, α-copaeno e o metil cis-isoeugenol. Também foi realizado o perfil fitoquímico por cromatografia em camada delgada (CCD), através da qual os constituintes fenólicos foram identificados como majoritários. Com o extrato bruto metanólico da própolis, realizou-se o ensaio de letalidade em Artemia salina, que demonstrou DL 50 de 18,9 µg/mL, sugerindo uma possível atividade antitumoral. Unitermos: Própolis vermelha, constituintes voláteis, Artemia salina.ABSTRACT: "Seasonal variability of red propolis constituents and brine shrimp bioassay". Propolis is a resinous hive product collected by honeybees from plant sources. The composition of the propolis depends on the season, the vegetation, and the area of collection. It presents a diverse biological activities such as antimicrobial, antioxidant and antitumoral. The seasonal variability of the red propolis constituents from Pernambuco State were accomplished in the months of February, June and October of 2006. The volatile red propolis constituients were captured by the dynamic headspace technique and analyzed by GC-MS. In the analysis of the samples, 34 constituents were identified as the mono and sesquiterpens, phenylpropanoids, aldehydes, cetons and n-alcanes, presenting as majority constituents the trans-anethol, α-copaene and methyl cis-isoeugenol. The phytochemistry profile was performed by thin layer chromatography (TLC). Through this technique the majority constituents were identified as phenols. The Brine shrimp bioassay was used to evaluate of the red propolis methanolic extract, which demonstrated a DL 50 of 18.9 µg/mL and an activity antitumoral was possibly suggested.

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“…It helps maintain aseptic conditions and appropriate temperature in the hive. The diversity of plants in the vicinity of the hive is the main factor determining the chemical composition of propolis 6,7 .…”

Section: Introductionmentioning

confidence: 99%

“…Green propolis, so called because of the presence of green pigment from the native shrub species Baccharis dracunculifolia 6 , is a Brazilian variety of propolis known for its many pharmacological properties. Among the most promising is the anticarcinogenic action of its constituents, some of which have been tested in laboratory animals and human cell culture [8][9][10] .…”

Section: Introductionmentioning

confidence: 99%

Chemoprevention with green propolis green propolis extracted in L-lysine versus carcinogenesis promotion with L-lysine in N-Butyl-N-[4-hydroxybutyl] nitrosamine (BBN) induced rat bladder cancer

Dornelas¹,

Fechine-Jamacaru²,

Albuquerque³

et al. 2012

Acta Cir. Bras.

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-ORIGINAL ARTICLEEXPERIMENTAL ABSTRACT PURPOSE:To determine the effects of green propolis extracted in L-lysine (WSDP) and of L-lysine for 40 weeks on induced rat bladder carcinogenesis. METHODS:The animals (groups I, II, III, IV, V and VI) received BBN during 14 weeks. Group I was treated with propolis 30 days prior received BBN, and then these animals were treated daily with propolis; Groups II and III was treated with subcutaneous and oral propolis (respectively) concurrently with BBN. The animals of Group IV were treated L-lysine; Group V received water subcutaneous;and Group VI received only to BBN. Among the animals not submitted to carcinogenesis induction, Group VII received propolis, Group VIII received L-lysine and Group IX received water. RESULTS:The carcinoma incidence in Group I was lower than that of control (Group VI). The carcinoma multiplicity in Group IV was greater than in Group VI. All animals treated with L-lysine developed carcinomas, and they were also more invasive in Group IV than in controls. On the other hand, Group VIII showed no bladder lesions. CONCLUSION:The WSDP is chemopreventive against rat bladder carcinogenesis, if administered 30 days prior to BBN , and that L-lysine causes promotion of bladder carcinogenesis.

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Isolation and Identification of Antimicrobial Compounds in Brazilian Propolis

Cited by 206 publications

References 0 publications

Factors that influence the yield and composition of Brazilian propolis extracts

Factors that influence the yield and composition of Brazilian propolis extracts

Variabilidade sazonal dos constituintes da própolis vermelha e bioatividade em Artermia salina

Chemoprevention with green propolis green propolis extracted in L-lysine versus carcinogenesis promotion with L-lysine in N-Butyl-N-[4-hydroxybutyl] nitrosamine (BBN) induced rat bladder cancer

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