ISOLATION AND IDENTIFICATION OF ANTIOXIDANT COMPOUNDS FROM METHANOL EXTRACT OF SAPPAN WOOD (Caesalpinia sappan)

Abstract

Sappan wood extract (Caesalpinia sappan) provides better antioxidant properties than ascorbic acid. Empirically, Sappan wood is often used as traditional medicine. To support the utilization of Sappan wood, the isolation and identification of active antioxidant compounds from the methanol extract of Sappan wood had been conducted from this study. Extraction was performed with maceration and evaporation methods. The spots of active antioxidant compounds in TLC-DPPH observations were isolated using liquid vacuum chromatography to obtain two pure compounds. The results of the LC-MS/MS identification of the two compounds, respectively, exhibited peak fragmentation of molecular ions at m/z 271.09, 257.15, 167.03, 151.06, 123.12, and 104.06 at the retention time of 6.35 minutes which were translated as Alpinetine and peak fragmentation of molecular ion at m/z 287.09, 177.06, 164.05, 123.04 and 110.04 at a retention time of 5.75. minutes which were translated as 3-Deoxysappanone B. The antioxidant activity quantitatively showed that Alpinetine and 3-Deoxysappanone B were capable of scavenging DPPH radicals with IC50 values ​​of 20.11 M and 15.28 M, respectively.

Caesalpinia sappan Linn. Ameliorates Allergic Nasal Inflammation by Upregulating the Keap1/Nrf2/HO-1 Pathway in an Allergic Rhinitis Mouse Model and Nasal Epithelial Cells

“…As shown in Figure 1 and Table 1 , episappanol (1), protosappanin B (2), sappanol (3), brazilin (4), 3-deoxysappanone B (5), and brazilein (6) were identified in CSLW. The retention time and mass spectra of protosappanin B were compared with reference standards (Targetmol, Wellesley Hills, MA, USA); five phytochemicals are consistent with those of previous studies [ 27 , 33 , 34 , 35 ].…”

Phytochemical Profile and Biological Activities of Ethylacetate Extract of Peanut (Arachis hypogaea L.) Stems: In-Vitro and In-Silico Studies with Bibliometric Analysis

“…(3-(3′,4′-Dihydroxybenzyl)-7hydroxychroman-4-one) (Yodha et al, 2021). The identified compounds are nine of fifteen major compounds from the EAE of A. hypogaea stems.…”

Antioxidant and antibacterial activity of sappan wood (Caesalpinia sappan L.) kombucha

“…This is influenced by the content of compounds in sappan wood that act as antioxidants. It was discovered that sappan wood contains phenolic and flavonoid compounds, which function as antioxidants (Yodha et al, 2021). Phenolic compounds in sappan wood include tannins (Kimestri et al, 2018) and Caesalpiniaphenol (Cuong et al, 2012).…”