Isolation and identification of antioxidative peptides from porcine collagen hydrolysate by consecutive chromatography and electrospray ionization–mass spectrometry

Лі, Бо; Chen, Feng; Wang, Xi; Ji, Baoping; Wu, Yufeng

doi:10.1016/j.foodchem.2006.07.002

Cited by 336 publications

(246 citation statements)

References 29 publications

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“…Leu has been reported to enhance the scavenging activities of peptides Li et al, 2007), as it has been also shown in this study. Furthermore, Gly and Pro have been suggested to play an important role in radical scavenging ability of some peptides (Mendis et al, 2005b;Chen et al, 1996;Rajapakse et al, 2005).…”

Section: Antioxidant Activity Of Synthetically Derived Peptidessupporting

confidence: 86%

“…Thus, Rajapkase et al (2005) reported two antioxidant peptides, Asn-Gly-Leu-Glu-Gly-Leu-Lys and Asn-Ala-AspPhe-Gly-Leu-Asn-Gly-Leu-Glu-Gly-Leu-Ala isolated from giant squid muscle protein. Li, Chen, Wang, Ji, and Wu (2007) identified the peptide Gln-Gly-Ala-Arg, which exhibited the highest antioxidant activity from porcine skin collagen hydrolysates.…”

Section: Antioxidant Activity Of Synthetically Derived Peptidesmentioning

confidence: 99%

“…The ABTS radical scavenging capacity observed in FIII may be attributed to other peptidic sequences with antiradical capacity although present in smaller quantities, together with the likely presence of glycosylated peptides. Other peptides isolated from skin gelatin hydrolysates of marine species have demonstrated noticeable free-radical scavenging activity (Mendis et al, 2005ab;Li et al, 2007).…”

Section: Antioxidant Activity Of Synthetically Derived Peptidesmentioning

confidence: 99%

“…For example, the presence of certain amino acids, such as His, Trp, Tyr, Phe, Met, Leu, Gly or Pro has been reported to enhance the scavenging activities of peptides (Chen, Muramoto, Yamauchi & Nokihara, 1996;Park, Jung, Nam, Shahidi & Kim, 2001; Hernández-Ledesma, Dávalos, Bartolomé & Amigo, 2005;Mendis, Rajapakse & Kim, 2005b;Li, Chen, Wang, Ji & Wu, 2007). Regarding the ACE-inhibitory activity, binding to ACE is strongly influenced by the C-terminal tripeptide sequence.…”

Section: Introductionmentioning

confidence: 99%

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Contribution of Leu and Hyp residues to antioxidant and ACE-inhibitory activities of peptide sequences isolated from squid gelatin hydrolysate

et al. 2011

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** This centre has implemented and maintains a Quality Management System which fulfils the requirements of the ISO standard 9001:2000 AbstractSquid gelatin obtained from inner and outer tunics was hydrolyzed with Alcalase to isolate antioxidant peptide sequences. The ACE-inhibitory activity of the isolated peptides was also evaluated. After fractionation by ultrafiltration and size-exclusion chromatography into four fractions, the antioxidant activity of the peptide fractions was determined by radical scavenging ability and ferric reducing power. Fraction FIII showed the highest antioxidant activity, although slight differences could be expected in the antioxidant activity of the different fractions based on the amino acid composition.FIII was subjected to liquid chromatography and tandem mass spectrometry (LC-MS/MS) and two major compounds were identified: the compound with m/z 952.42, which could be mostly comprised by the carbohydrate fucose, and the peptide with m/z 1410.63. Three possible sequences were proposed for this peptide, and the contribution of Leu or Hyp residues to the antioxidant and ACE-inhibitory activities of the resulting sequence was evaluated. The presence of Leu residues in the peptide sequence in *Manuscript Click here to view linked References 2 replacement of Hyp seems to play an important role in the antioxidant and ACEinhibitory activity.

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Section: Antioxidant Activity Of Synthetically Derived Peptidessupporting

confidence: 86%

Section: Antioxidant Activity Of Synthetically Derived Peptidesmentioning

confidence: 99%

Section: Antioxidant Activity Of Synthetically Derived Peptidesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Contribution of Leu and Hyp residues to antioxidant and ACE-inhibitory activities of peptide sequences isolated from squid gelatin hydrolysate

et al. 2011

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“…Histidine has the proton-donation ability, while methionine is prone to oxidation of the methionine sulfoxide, and cysteine donates the sulfur hydrogen (Herna'ndez- Ledesma et al, 2005;Tsuge et al, 1991). Some recent reports suggested that the peptide Gln-Gly-Ala-Arg, which exhibited the highest antioxidant activity from porcine skin collagen hydrolysates (Li et al, 2007;Suetsuna et al, 2000), and the Glu-Leu residue in peptides was recently reported to play an important role in radical scavenging (Jun et al, 2004). Additionally, presence of aspartic acid seems to play a vital role irrespective of its position as observed in several antioxidative peptide sequences (Byuna et al, 2009).…”

Section: Amino Acid Composition Of Chungkukjang and Their Antioxidantmentioning

confidence: 99%

Evaluation of Antioxidant Activities of Peptides Isolated from Korean Fermented Soybean Paste, Chungkukjang

Kim¹,

Chi²,

Kim³

et al. 2011

Korean Journal of Crop Science

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The objectives of present study were to characterize the peptides which were isolated from Korean fermented soybean paste, chungkukjang, and to determine their antioxidant activities. Four fractions were collected from the methanol extract of chungkukjang by using a recycling preparative HPLC. Among fractions, Fr-2 was identified to be highly potent free radical scavenging activity in the assay of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and nitroblue tetrazolium(NBT)-reduction inhibition. Base on antioxidant effects, fraction Fr-2 was employed for the refraction with a prep-column and separated into five fractions of which two fractions were identified to have higher antioxidant activity. To confirm the amino acid constituents of antioxidant fractions Fr-2-2 and Fr-2-3 were analyzed, and eight kinds of amino acids such as aspartic acid, threonine, serine, glutamic acid, glycine, lysine, histidine, and arginine were identified as the constituent amino acids. Antioxidant activities of the separated peptides were further assessed cell viability with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl terazolium bromide (MTT), and fluorescence-activated cell sorting (FACS) analysis of H4IIE cells treated with hydrogen peroxide (H 2 O 2 ). Chungkukjang peptides have shown their ability to protect H4IIE rat hepatoma cells against H 2 O 2 -induced oxidative stress by concentration and time-dependent manner. Therefore, These results indicated that fermented soybean paste chungkukjang will be promoted the antioxidant and radical scavenging activities, and beneficial for health. The antioxidant peptide fractions Fr-2-2 and Fr-2-3 were denominated as P-NICS-1 and P-NICS-2, respectively. However, further studies were required to clarify their amino acid sequences and molecular properties, and physiological significances.

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Production and Antioxidant Properties of Marine‐derived Bioactive Peptides

Wang¹,

Zhao²,

Wang³

2013

Marine Proteins and Peptides

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Isolation and identification of antioxidative peptides from porcine collagen hydrolysate by consecutive chromatography and electrospray ionization–mass spectrometry

Cited by 336 publications

References 29 publications

Contribution of Leu and Hyp residues to antioxidant and ACE-inhibitory activities of peptide sequences isolated from squid gelatin hydrolysate

Contribution of Leu and Hyp residues to antioxidant and ACE-inhibitory activities of peptide sequences isolated from squid gelatin hydrolysate

Evaluation of Antioxidant Activities of Peptides Isolated from Korean Fermented Soybean Paste, Chungkukjang

Production and Antioxidant Properties of Marine‐derived Bioactive Peptides

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